LOCUS       sequence-103958-         715 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  MuLE (Multiple Lentiviral Expression) Destination vector for use 
            with pMuLE Entry vectors. Co-expresses firefly luciferase under the 
            control of a PGK promoter. Compatible with MultiSite Gateway 
            cloning.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 715)
  AUTHORS   Albers J, Danzer C, Rechsteiner M, Lehmann H, Brandt LP, Hejhal T, 
            Catalano A, Busenhart P, Goncalves AF, Brandt S, Bode PK, 
            Bode-Lesniewska B, Wild PJ, Frew IJ
  TITLE     A versatile modular vector system for rapid combinatorial mammalian 
            genetics.
  JOURNAL   J Clin Invest. 2015 Mar 9. pii: 79743. doi: 10.1172/JCI79743.
  PUBMED    25751063
REFERENCE   2  (bases 1 to 715)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Clin 
            Invest. 2015 Mar 9. pii: 79743. doi: 10.1172/JCI79743."
COMMENT     Sequence Label: pMuLE Lenti Dest Luc2
FEATURES             Location/Qualifiers
     source          1..715
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     LTR             75..308
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
ORIGIN
        1 gttcctctca ctctctgata ttcatttctt tgcaagttat aaatactgaa taataagatg
       61 acatgaacta ctactgctag agattttcca cactgactaa aagggtctga gggatctcta
      121 gttaccagag tcacacaaca gacgggcaca cactacttga agcactcaag gcaagcttta
      181 ttgaggctta agcagtgggt tccctagtta gccagagagc tcccaggctc agatctggtc
      241 taaccagaga gacccagtac aagcaaaaag cagatcttgt cttcgttggg agtgagctag
      301 cccttccagt cccccctttt cttttaaaaa gtggctaaga tctacagctg ccttgtaagt
      361 cattggtctt aaaggtaccg ggccctcgag atgcatacgc gtcggcagcc ccgactctag
      421 aattattaca cggcgatctt gccgcccttc ttggccttaa tgagaatctc gcggatcttg
      481 cgggcgtcca acttgccggt cagtccttta ggcacctcgt ccacgaacac aacaccaccg
      541 cgcagcttct tggcggttgt aacctggctg gccacatagt ccacgatctc cttctcggtc
      601 atggttttac cgtgttccag cacgacgact gcggcgggca gctcgccggc atcgtcgtcg
      661 ggcaggccgg cgaccccggc gtcgaagatg ttggggtgtt gcagcaggat gctct
//
