LOCUS       sequence-103962-        3902 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  full length human VARP with C-terminal EGFP tag cloned into pLXIN.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3902)
  AUTHORS   Hesketh GG, Perez-Dorado I, Jackson LP, Wartosch L, Schafer IB, Gray
            SR, McCoy AJ, Zeldin OB, Garman EF, Harbour ME, Evans PR, Seaman MN,
            Luzio JP, Owen DJ
  TITLE     VARP is recruited on to endosomes by direct interaction with 
            retromer, where together they function in export to the cell 
            surface.
  JOURNAL   Dev Cell. 2014 Jun 9;29(5):591-606. doi: 
            10.1016/j.devcel.2014.04.010. Epub 2014 May 22.
  PUBMED    24856514
REFERENCE   2  (bases 1 to 3902)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2014.04.010"; journalName: "Dev Cell"; date: 
            "2014-06-9- 9"; volume: "29"; issue: "5"; pages: "591-606"
COMMENT     Sequence Label: VARP GFP-pLXIN
FEATURES             Location/Qualifiers
     source          1..3902
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      3168..3177
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3174..3176,3177..3179,3180..3893)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3174..3176,3177..3179,3180..3890)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3219..3240)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3480..3499)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3827..3848
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ctcgagccat ggctctgtat gatgaagacc tcctgaaaaa tcctttctat ctggctctgc
       61 aaaagtgccg ccctgacttg tgcagcaaag tggcccaaat ccatggcatt gtcttagtac
      121 cctgcaaagg aagcctgtcg agcagcatcc agtctacttg tcagtttgag tcctacattt
      181 tgatacctgt ggaagagcat tttcagacct taaatggaaa ggatgtcttt attcaaggga
      241 acaggattaa attaggagct ggttttgcct gtcttctctc agtgcccatt ctctttgaag
      301 aaactttcta caatgaaaaa gaagagagtt tcagcatcct gtgtatagcc catcctttgg
      361 aaaagagaga gagttcagaa gagcctttgg caccctcaga tcccttttcc ctgaaaacca
      421 ttgaagatgt gagagagttc ttgggaagac actccgagcg atttgacagg aacatcgcct
      481 ctttccatcg aacattccga gaatgcgaga gaaagagcct ccgtcaccac atagactcag
      541 cgaatgctct ctacaccaaa tgcctccagc agcttctgag ggactctcac ctgaaaatgc
      601 tcgccaagca ggaggcccag atgaacctga tgaagcaggc agtggagata tacgtccatc
      661 atgaaattta caacctgatc tttaaatacg tggggaccat ggaggcaagt gaggatgcgg
      721 cctttaacaa aatcacaaga agccttcaag atcttcagca gaaagatatt ggtgtgaaac
      781 cggagttcag ctttaacata cctcgtgcca aaagagagct ggctcagctg aacaaatgca
      841 cctccccaca gcagaagctt gtctgcttgc gaaaagtggt gcagctcatt acacagtctc
      901 caagccagag agtgaacctg gagaccatgt gtgctgatga tctgctatca gtcctgttat
      961 acttgcttgt gaaaacggag atccctaatt ggatggcaaa tttgagttac atcaaaaact
     1021 tcaggtttag cagcttggca aaggatgaac tgggatactg cctgacctca ttcgaagctg
     1081 ccattgaata tattcggcaa ggaagcctct ctgctaaacc ccctgagtct gagggatttg
     1141 gagacaggct gttccttaag cagagaatga gcttactctc tcagatgact tcgtctccca
     1201 ccgactgcct gtttaagcac attgcatcag gtaaccagaa agaagtggag agacttctga
     1261 gccaagagga ccacgacaag gacactgtac agaagatgtg tcaccctctc tgcttctgcg
     1321 atgactgtga gaaactcgtc tctgggaggt tgaatgatcc ctcagttgtc actccattct
     1381 ccagagacga cagggggcac acccctctcc atgtggctgc tgtctgtggg caggcatccc
     1441 tcatcgacct cctggtttcc aagggcgcca tggtaaatgc cacagactac catggagcca
     1501 ctccgctcca cctggcctgt cagaagggct accagagcgt gacgctgctg ctgctgcact
     1561 acaaggccag cgcggaagtg caggacaaca atgggaatac gccactccac ctggcctgca
     1621 cctacggcca cgaggactgt gtgaaggctc tggtttacta cgacgtggag tcgtgcagac
     1681 ttgacattgg caatgagaaa ggagacaccc ctctacacat tgctgcccgc tggggctacc
     1741 aaggcgtcat agagacattg ctgcagaacg gagcgtccac cgagatccag aacagactga
     1801 aggagacgcc cctcaagtgt gcattaaact caaagattct gtctgtaatg gaagcctatc
     1861 acctgtcctt cgagaggagg cagaagtcgt ccgaggcccc tgtgcagtcc ccgcagcgct
     1921 ccgtggactc catcagccaa gagtcctcca cttccagctt ctcctccatg tcagccagct
     1981 caaggcagga ggagaccaag aaggactaca gagaggtaga aaaacttttg agagcagttg
     2041 ctgatggaga tctagaaatg gtgcgttacc tgttggaatg gacagaggag gacctggagg
     2101 atgcggagga cactgtcagt gcagcggacc ccgaattctg tcacccgttg tgccagtgcc
     2161 ccaagtgtgc cccagctcag aagaggctgg cgaaggttcc tgccagtggg cttggtgtga
     2221 acgtgaccag ccaggacggc tcctccccgc tgcatgtcgc cgccctgcac ggccgggcgg
     2281 acctcatccc cctcctgctg aagcacgggg ccaacgcagg tgccaggaac gcagaccaag
     2341 ccgtcccgct ccacctggcc tgccagcagg gccactttca ggtggtgaag tgtctgttag
     2401 attcgaatgc aaaacccaat aagaaggacc tcagtggaaa cacgcccctc atttacgcct
     2461 gctccggtgg ccatcacgag cttgtggcac tgctgctaca gcacggggcc tccattaacg
     2521 cttctaacaa taagggcaac acagcgctgc acgaggctgt gattgaaaag cacgtcttcg
     2581 tggtagagct gcttctgctc cacggagcgt cagttcaggt gctgaacaag cggcagcgca
     2641 cggctgtaga ctgtgctgaa cagaattcaa aaataatgga attgcttcag gtggtaccaa
     2701 gctgtgttgc ttcattagat gatgtggctg aaactgaccg caaggagtat gtcactgtta
     2761 agatcaggaa aaaatggaac tcaaaactgt atgatctacc agatgagcct tttacaagac
     2821 agttttactt tgtccactca gctggtcagt ttaagggaaa gacttcaagg gagattatgg
     2881 caagagatag aagtgtccct aatttaaccg aaggttcttt gcatgagcca gggaggcaaa
     2941 gtgtcacact gagacagaat aacctgccag ctcagagtgg atctcatgct gctgagaaag
     3001 gcaacagcga ctggccagag aggcctggac tgacacagac tggccctgga cacagacgga
     3061 tgctgcggag acacacggta gaggatgcgg tcgtgtccca gggcccggag gctgctggcc
     3121 ccctctccac tccccaagag gttagtgctt cccggtccgg atccatcgcc accatggtga
     3181 gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg
     3241 taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc
     3301 tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga
     3361 ccaccctgac ctacggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg
     3421 acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg
     3481 acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc
     3541 gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg
     3601 agtacaacta caacagccac aacgtctata tcatggccga caagcagaag aacggcatca
     3661 aggtgaactt caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact
     3721 accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga
     3781 gcacccagtc cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg
     3841 agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag taaagcggcc
     3901 gc
//
