LOCUS       sequence-103984-         903 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleus/Histones, Excitation: 587, Emission: 610.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 903)
  AUTHORS   Kuipers MA, Stasevich TJ, Sasaki T, Wilson KA, Hazelwood KL, McNally
            JG, Davidson MW, Gilbert DM
  TITLE     Highly stable loading of Mcm proteins onto chromatin in living cells
            requires replication to unload.
  JOURNAL   J Cell Biol. 2011 Jan 10;192(1):29-41. doi: 10.1083/jcb.201007111.
  PUBMED    21220507
REFERENCE   2  (bases 1 to 903)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1083/jcb"; 
            journalName: "J Cell Biol"; date: "2011-01-10- 10"; volume: "192"; 
            issue: "1"; pages: "29-41"
COMMENT     Sequence Label: mCherry-H2B-6
FEATURES             Location/Qualifiers
     source          1..903
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      103..112
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(125..502)
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     promoter        complement(591..794)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(620..640)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 cctcgatctc gaactcgtgg ccgttcacgg agccctccat gtgcaccttg aagcgcatga
       61 actccttgat gatggccatg ttatcctcct cgcccttgct caccatggtg gcgaccggtg
      121 gatccttagc gctggtgtac ttggtgatgg ccttagtacc ctcggacacg gcgtgcttgg
      181 ccaactcccc aggcagcagc aggcgcacgg ccgtctggat ctccctggag gtgatggtcg
      241 agcgcttgtt gtaatgcgcc aggcgggaag cctcacctgc gatgcgctcg aaaatgtcgt
      301 tcacaaacga attcatgatg cccatggcct tggacgaaat gccggtgtca gggtggacct
      361 gcttcagaac cttgtacaca tagatggaat agctctcctt gcggctgcgc ttgcgcttct
      421 tgccgccttt cttctgcgcc ttagtcaccg ccttcttgga gccctttttc ggggcgggag
      481 cagacttcgc tggctctggc atggtggcgg taccgtcgac tgcagaattc gaagcttgag
      541 ctcgagatct gagtccggta gcgctagcgg atctgacggt tcactaaacc agctctgctt
      601 atatagacct cccaccgtac acgcctaccg cccatttgcg tcaatggggc ggagttgtta
      661 cgacattttg gaaagtcccg ttgattttgg tgccaaaaca aactcccatt gacgtcaatg
      721 gggtggagac ttggaaatcc ccgtgagtca aaccgctatc cacgcccatt gatgtactgc
      781 caaaaccgca tcaccatggt aatagcgatg actaatacgt agatgtactg ccaagtagga
      841 aagtcccata aggtcatgta ctgggcataa tgccaggcgg gccatttacc gtcattgacg
      901 tca
//
