LOCUS       sequence-103986-         832 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of human MDM2 (1-120) in e. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 832)
  AUTHORS   Borcherds W, Theillet FX, Katzer A, Finzel A, Mishall KM, Powell AT,
            Wu H, Manieri W, Dieterich C, Selenko P, Loewer A, Daughdrill GW
  TITLE     Disorder and residual helicity alter p53-Mdm2 binding affinity and 
            signaling in cells.
  JOURNAL   Nat Chem Biol. 2014 Dec;10(12):1000-2. doi: 10.1038/nchembio.1668. 
            Epub 2014 Nov 2.
  PUBMED    25362358
REFERENCE   2  (bases 1 to 832)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nchembio.1668"; journalName: "Nat Chem Biol"; date: 
            "2014-12"; volume: "10"; issue: "12"; pages: "1000-2"
COMMENT     Sequence Label: pGEX6P2-MDMX (1-120)
FEATURES             Location/Qualifiers
     source          1..832
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             12..35
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     primer_bind     complement(488..510)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(655..673)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tctcaatcgg actggaagtt ctgttccagg ggcccctggg atccatgaca tcattttcca
       61 cctctgctca gtgttcaaca tctgacagtg cttgcaggat ctctcctgga caaatcaatc
      121 aggtacgacc aaaactgccg cttttgaaga ttttgcatgc agcaggtgcg caaggtgaaa
      181 tgttcactgt taaagaggtc atgcactatt taggtcagta cataatggtg aagcaacttt
      241 atgatcagca ggagcagcat atggtatatt gtggtggaga tcttttggga gaactactgg
      301 gacgtcagag cttctccgtg aaagacccaa gccctctcta tgatatgcta agaaagaatc
      361 ttgtcacttt agccactgct actacagatg ctgctcagac tctctaagaa ttcccgggtc
      421 gactcgagcg gccgcatcgt gactgactga cgatctgcct cgcgcgtttc ggtgatgacg
      481 gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg
      541 ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggcgcag
      601 ccatgaccca gtcacgtagc gatagcggag tgtataattc ttgaagacga aagggcctcg
      661 tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg
      721 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
      781 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tg
//
