LOCUS       sequence-104010-         876 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 876)
  AUTHORS   Carey KL, Richards SA, Lounsbury KM, Macara IG
  TITLE     Evidence using a green fluorescent protein-glucocorticoid receptor 
            chimera that the Ran/TC4 GTPase mediates an essential function 
            independent of nuclear protein import.
  JOURNAL   J Cell Biol. 1996 Jun . 133(5):985-96.
  PUBMED    8655589
REFERENCE   2  (bases 1 to 876)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol. 1996 Jun . 133(5):985-96."
COMMENT     Sequence Label: pk7-GR-GFP
FEATURES             Location/Qualifiers
     source          1..876
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(421..440)
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        complement(453..471)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(454..471)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(681..705)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(702..801)
                     /label=CMVd1 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields slightly reduced expression 
                     relative to the full-length promoter"
     primer_bind     complement(731..751)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 aagctttaag tctgtttccc ccgaggaaag gctgatttgg ccctgctgtg ggaatcccag
       61 gtcatttccc atcacttttg tttctgtctc tcccatatac agtcccattg agagtgaaac
      121 tgctttggac agatctggct gctgcgcatt gcttactgag ccttttggaa aatcaaccaa
      181 aagtcttcgc tgcttggagt ctgattgaga agcgacagcc agtgagggtg aagacgcaga
      241 aaccttcaca gtagctcctc ctcttagggt tttatagaag tccatcacat ctcccctctc
      301 ctgagcaagc acactgctgg ggttttcttc tctaccagga gttaatgatt ctttggagtc
      361 cattctagag tcgacctgca gaagcttaga accgaggtgc agttggacct gggagtggac
      421 acctgtggag agaaaggcaa agtggatgtt attctatagt gtcacctaaa tcgtatgtgt
      481 atgatacata aggttatgta ttaattgtag ccgcgttcta acgaagccaa gggggtgggc
      541 ctatagactc tataggcggt acttacgtca ctcttggcac ggggaatccg cgttccaatg
      601 caccgttccc ggccgcggag gctggatcgg tcccggtgtc ttctatggag gtcaaaacag
      661 cgtggatggc gtctccaggc gatctgacgg ttcactaaac gagctctgct tatatagacc
      721 tcccaccgta cacgcctacc gcccatttgc gtcaatgggg cggagttgtt acgacatttt
      781 ggaaagtccc gttgattttg gtgccaaaac aaactcccat tgacgtcaat ggggtggaga
      841 cttggaaatc cccgtgagtc aaaccgctat ccacgc
//
