LOCUS       sequence-104025-         896 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  IncP-based replicating vector expressing dTomato under tac 
            promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 896)
  AUTHORS   Puri AW, Owen S, Chu F, Chavkin T, Beck DA, Kalyuzhnaya MG, Lidstrom
            ME
  TITLE     Genetic tools for the industrially promising methanotroph 
            Methylomicrobium buryatense.
  JOURNAL   Appl Environ Microbiol. 2014 Dec 29. pii: AEM.03795-14.
  PUBMED    25548049
REFERENCE   2  (bases 1 to 896)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl 
            Environ Microbiol. 2014 Dec 29. pii: AEM.03795-14."
COMMENT     Sequence Label: pAWP89
FEATURES             Location/Qualifiers
     source          1..896
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        join(195..200,201..216,217..223)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     primer_bind     complement(385..403)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     653..672
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     847..870
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
       61 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
      121 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctgc
      181 tctgaaatga gctgttgaca attaatcatc ggctcgtata atgtgtggag gtattcacac
      241 aggaaacagc tatggtgagc aagggcgagg aggtcatcaa agagttcatg cgcttcaagg
      301 tgcgcatgga gggctccatg aacggccacg agttcgagat cgagggcgag ggcgagggcc
      361 gcccctacga gggcacccag accgccaagc tgaaggtgac caagggcggc cccctgccct
      421 tcgcctggga catcctgtcc ccccagttca tgtacggctc caaggcgtac gtgaagcacc
      481 ccgccgacat ccccgattac aagaagctgt ccttccccga gggcttcaag tgggagcgcg
      541 tgatgaactt cgaggacggc ggtctggtga ccgtgaccca ggactcctcc ctgcaggacg
      601 gcacgctgat ctacaaggtg aagatgcgcg gcaccaactt cccccccgac ggccccgtaa
      661 tgcagaagaa gaccatgggc tgggaggcct ccaccgagcg cctgtacccc cgcgacggcg
      721 tgctgaaggg cgagatccac caggccctga agctgaagga cggcggccac tacctggtgg
      781 agttcaagac catctacatg gccaagaagc ccgtgcaact gcccggctac tactacgtgg
      841 acaccaagct ggacatcacc tcccacaacg aggactacac catcgtggaa cagtac
//
