LOCUS       sequence-104038-        1095 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pCS2 with antifreeze protein terminator instead of sv40.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1095)
  AUTHORS   Horstick EJ, Jordan DC, Bergeron SA, Tabor KM, Serpe M, Feldman B, 
            Burgess HA
  TITLE     Increased functional protein expression using nucleotide sequence 
            features enriched in highly expressed genes in zebrafish.
  JOURNAL   Nucleic Acids Res. 2015 Jan 27. pii: gkv035.
  PUBMED    25628360
REFERENCE   2  (bases 1 to 1095)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2015 Jan 27. pii: gkv035."
COMMENT     Sequence Label: pCS2z
FEATURES             Location/Qualifiers
     source          1..1095
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        63..81
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     63..80
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     regulatory      140..149
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(637..657)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(637..655)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(676..692)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(676..692)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(689..711)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    700..716
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(724..730,731..748,749..754))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    769..790
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(907..924)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ctatataagc aatgctcgtt tagggaaccg ccattctgcc tggggacgtc ggagcaagct
       61 tgatttaggt gacactatag aatacaagct acttgttctt tttgcaggat cccatcgatt
      121 cgaattcaag gcctctagag ccaccatggg gtaactcgag cctctagtaa ttaagtctca
      181 gccaccgtta actgaacatg tcaaaacctg tggagactgt tgagatttga tgttctgaaa
      241 agataaagcc tataaataaa atgttgccca aatttcctgc ctgatgtttt tctttgtctt
      301 tgctacatgg ctttgctgct cggatcggct cactctgtgt atgccacgtt cactttgtac
      361 tctccttctc acggtaggtt tattattttt agatgtgcag ttagtttctg tgaaataaca
      421 caccacacac tgatattgtc tgtgcattga cttggtgagt gcacattgtt tttgatcttg
      481 acatatttat atttgattga tcaggtgaac tgtgtgaatc taaagtgctc catacagatg
      541 ttctgcattg aaaatattct cattttatta gtggaagtga gtgtatgcca gcggccgcgg
      601 cgccaatgca ttgggcccgg tacccagctt ttgttccctt tagtgagggt taattgcgcg
      661 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
      721 acacaacata cgagccggga gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
      781 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
      841 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
      901 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
      961 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     1021 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     1081 ccataggctc cgccc
//
