LOCUS       sequence-104056-         964 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Cas9 under control of nanos promoter and 3'UTR. For germ 
            line restricted genome engineering in Drosophila melanogaster..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 964)
  AUTHORS   Port F, Chen HM, Lee T, Bullock SL
  TITLE     Optimized CRISPR/Cas tools for efficient germline and somatic genome
            engineering in Drosophila.
  JOURNAL   Proc Natl Acad Sci U S A. 2014 Jul 7. pii: 201405500.
  PUBMED    25002478
REFERENCE   2  (bases 1 to 964)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2014 Jul 7. pii: 201405500."
COMMENT     Sequence Label: pnos-Cas9-nos
FEATURES             Location/Qualifiers
     source          1..964
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    158..191
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 actgaaagtg acatccagtg tttgttcctt gtgtagatgc atctcaaaaa aatggtgggc
       61 ataatagtgt tgtttatata tatcaaaaat aacaactata ataataagaa tacatttaat
      121 ttagaaaatg cttggatttc actggaacta ggctagcata acttcgtata atgtatgcta
      181 tacgaagtta tgctagcgga tccaagcttc gaccgtttta acctcgaaat atgcacatgt
      241 aaggacggat gtgagcgaac gccagtgatg accgggatca gaggtaacct accatgcatg
      301 gtggggatta ggtgaccgtt cgcaggtagt ttgatcggag cgaatgttcg gggggtctgg
      361 cgtcagaggc tctaaacttt atgtaattcc tgccgcgaaa cacgcacgta tcaagcagtc
      421 agctgttctc ttcgttcagc gcgcgccggt gttgcaaaac gagcgctctt cgccggcggt
      481 ggctcgtgcg atagttcgtt ttgtcggtaa tccgatgttg ccgcgccgat atcatgtgat
      541 gttgtcacag tgcgcgaaat tcgaatggtg gtgtgcagtg attgtgttgt gacggcgagt
      601 ggcgcgtgtg ggtgcttagt tttgggagat gttttcgtat ttttttgttg ataactcagg
      661 ctttgttgct gtgttgtagt actattttcc attgcgcggt gtccagcttt taattagtgg
      721 cacatattct tagcaagtaa aaattatttt gcatactatt aaatttctta taaattattt
      781 tctaaaatta agtttacctt ttcaatttta ctaaaaatat cgatatattt attatcgctg
      841 gaaaactaca ttattccacc tctaagcaag aaccgttagt tggcgcgtag ctttaccaca
      901 aaattcctgg aattgccgta cgcttcgcag ttgtttcaag ttgtctaagg gacatacgat
      961 tttt
//
