LOCUS       sequence-104057-         879 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Cas9 under control of nanos promoter and 3'UTR. For germ 
            line restricted genome engineering in Drosophila melanogaster..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 879)
  AUTHORS   Port F, Chen HM, Lee T, Bullock SL
  TITLE     Optimized CRISPR/Cas tools for efficient germline and somatic genome
            engineering in Drosophila.
  JOURNAL   Proc Natl Acad Sci U S A. 2014 Jul 7. pii: 201405500.
  PUBMED    25002478
REFERENCE   2  (bases 1 to 879)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2014 Jul 7. pii: 201405500."
COMMENT     Sequence Label: pnos-Cas9-nos
FEATURES             Location/Qualifiers
     source          1..879
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             63..83
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ttacggggct ctatgaaaca agaatcgacc tctctcagct cggtggagac agcagggctg
       61 accccaagaa gaagaggaag gtgtgagcga atccagctct ggagcagagg ctctggcagc
      121 ttttgcagcg tttatataac atgaaatata tatacgcatt ccgatcaaag ctgggttaac
      181 cagatagata gtaacgttta actagcgcct ggcgcgttcg attttaaaga gatttagagc
      241 gttatcccgt gcctatagat cttatagtat agacaacgaa cgatcactca aatccaagtc
      301 aataattcaa gaatttatgt ctgtttctgt gaaagggaaa ctaattttgt taaagaagac
      361 ttacaatatc gtaatacttg ttcaatcgtc gtggccgata gaaatatctt acaatccgaa
      421 agttgatgaa tggaattggt ctgcaactgg tcgccttcat tttgtaaaat gttcgcttgc
      481 ggccgaaaaa tttcgatata tctacaattg atatacaatc tttactaaat tttgaaaaag
      541 gaacactttg aatttcgaac tgtcaatcgt atcattagaa tttaatctaa atttaaatct
      601 tgctaaagga aatagcaagg aacactttcg tcgtcggcta cgcattcatt gtaaaatttt
      661 aaattttgac attccgcact ttttgataga taagcgaaga gtatttttat tacatgtatc
      721 gcaagtattc atttcaacac acatatctat atatatatgt tatatattta ttcaattttg
      781 tttaccattg atcaattttt cacacatgaa acaaccgcca gcattatata atttttttat
      841 ttttttaaaa aatgtgtaca catattctga aaatgaaaa
//
