LOCUS       sequence-104059-         804 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Cas9 under control of nanos promoter and 3'UTR. For germ 
            line restricted genome engineering in Drosophila melanogaster..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 804)
  AUTHORS   Port F, Chen HM, Lee T, Bullock SL
  TITLE     Optimized CRISPR/Cas tools for efficient germline and somatic genome
            engineering in Drosophila.
  JOURNAL   Proc Natl Acad Sci U S A. 2014 Jul 7. pii: 201405500.
  PUBMED    25002478
REFERENCE   2  (bases 1 to 804)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2014 Jul 7. pii: 201405500."
COMMENT     Sequence Label: pnos-Cas9-nos
FEATURES             Location/Qualifiers
     source          1..804
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 tcctccagcc tatggaagaa agagtcatcc accttagcca tctcattact aaagatctcc
       61 tgcaggtagc agatccgatt ctttctgcgg gtatatctgc gccgtgctgt tcttttgagc
      121 cgcgtggctt cggccgtctc cccggagtcg aacaggaggg cgccaatgag gttcttcttt
      181 atgctgtggc gatcggtatt gcccagaact ttgaattttt tgctcggcac cttgtactcg
      241 tccgtaatga cggcccagcc gacgctgttt gtgccgatat cgagcccaat ggagtacttc
      301 ttgtccatgg cgaaaatccg ggtcgaaagt tacggttatc gcgcactcta ctttccacaa
      361 atcctcaccc aaaaaccaag cacagtttat tcaactgaag tattcgcgat acttctttat
      421 ctaataataa tgtacatgta actaaactcg cttttgggtt aaaatcgtga cgcagaggca
      481 aaaaaaatcg tatgtccctt agacaacttg aaacaactgc gaagcgtacg gcaattccag
      541 gaattttgtg gtaaagctac gcgccaacta acggttcttg cttagaggtg gaataatgta
      601 gttttccagc gataataaat atatcgatat ttttagtaaa attgaaaagg taaacttaat
      661 tttagaaaat aatttataag aaatttaata gtatgcaaaa taatttttac ttgctaagaa
      721 tatgtgccac taattaaaag ctggacaccg cgcaatggaa aatagtacta caacacagca
      781 acaaagcctg agttatcaac aaaa
//
