LOCUS       sequence-104060-         965 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Fok1:dCas9 under control of nanos promoter and 3'UTR. For 
            germ line restricted high specificity genome engineering in 
            Drosophila melanogaster..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 965)
  TITLE     CRISPRflydesign
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 965)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pnos-Fok1:dCas9-nos
FEATURES             Location/Qualifiers
     source          1..965
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    157..190
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 ctgaaagtga catccagtgt ttgttccttg tgtagatgca tctcaaaaaa atggtgggca
       61 taatagtgtt gtttatatat atcaaaaata acaactataa taataagaat acatttaatt
      121 tagaaaatgc ttggatttca ctggaactag gctagcataa cttcgtataa tgtatgctat
      181 acgaagttat gctagcggat ccaagcttcg accgttttaa cctcgaaata tgcacatgta
      241 aggacggatg tgagcgaacg ccagtgatga ccgggatcag aggtaaccta ccatgcatgg
      301 tggggattag gtgaccgttc gcaggtagtt tgatcggagc gaatgttcgg ggggtctggc
      361 gtcagaggct ctaaacttta tgtaattcct gccgcgaaac acgcacgtat caagcagtca
      421 gctgttctct tcgttcagcg cgcgccggtg ttgcaaaacg agcgctcttc gccggcggtg
      481 gctcgtgcga tagttcgttt tgtcggtaat ccgatgttgc cgcgccgata tcatgtgatg
      541 ttgtcacagt gcgcgaaatt cgaatggtgg tgtgcagtga ttgtgttgtg acggcgagtg
      601 gcgcgtgtgg gtgcttagtt ttgggagatg ttttcgtatt tttttgttga taactcaggc
      661 tttgttgctg tgttgtagta ctattttcca ttgcgcggtg tccagctttt aattagtggc
      721 acatattctt agcaagtaaa aattattttg catactatta aatttcttat aaattatttt
      781 ctaaaattaa gtttaccttt tcaattttac taaaaatatc gatatattta ttatcgctgg
      841 aaaactacat tattccacct ctaagcaaga accgttagtt ggcgcgtagc tttaccacaa
      901 aattcctgga attgccgtac gcttcgcagt tgtttcaagt tgtctaaggg acatacgatt
      961 ttttt
//
