LOCUS       sequence-104061-         495 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Fok1:dCas9 under control of nanos promoter and 3'UTR. For 
            germ line restricted high specificity genome engineering in 
            Drosophila melanogaster..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 495)
  TITLE     CRISPRflydesign
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 495)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pnos-Fok1:dCas9-nos
FEATURES             Location/Qualifiers
     source          1..495
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             53..73
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ctatgaaaca agaatcgacc tctctcagct cggtggagac agcagggctg accccaagaa
       61 gaagaggaag gtgtgagcga atccagctct ggagcagagg ctctggcagc ttttgcagcg
      121 tttatataac atgaaatata tatacgcatt ccgatcaaag ctgggttaac cagatagata
      181 gtaacgttta actagcgcct ggcgcgttcg attttaaaga gatttagagc gttatcccgt
      241 gcctatagat cttatagtat agacaacgaa cgatcactca aatccaagtc aataattcaa
      301 gaatttatgt ctgtttctgt gaaagggaaa ctaattttgt taaagaagac ttacaatatc
      361 gtaatacttg ttcaatcgtc gtggccgata gaaatatctt acaatccgaa agttgatgaa
      421 tggaattggt ctgcaactgg tcgccttcat tttgtaaaat gttcgcttgc ggccgaaaaa
      481 tttcgatata tctac
//
