LOCUS       sequence-104063-        1001 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Fok1:dCas9 under control of nanos promoter and 3'UTR. For 
            germ line restricted high specificity genome engineering in 
            Drosophila melanogaster..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1001)
  TITLE     CRISPRflydesign
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1001)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pnos-Fok1:dCas9-nos
FEATURES             Location/Qualifiers
     source          1..1001
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(321..908)
                     /codon_start=1
                     /product="nonspecific DNA cleavage domain of the FokI 
                     endonuclease (Li et al., 1992)"
                     /label=FokI cleavage domain
                     /note="must dimerize to be catalytically active"
                     /translation="QLVKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKV
                     MEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMQR
                     YVEENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNCNGAVL
                     SVEELLIGGEMIKAGTLTLEEVRRKFNNGEINF"
     CDS             complement(915..935)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 tcctccagcc tatggaagaa agagtcatcc accttagcca tctcattact aaagatctcc
       61 tgcaggtagc agatccgatt ctttctgcgg gtatatctgc gccgtgctgt tcttttgagc
      121 cgcgtggctt cggccgtctc cccggagtcg aacaggaggg cgccaatgag gttcttcttt
      181 atgctgtggc gatcggtatt gcccagaact ttgaattttt tgctcggcac cttgtactcg
      241 tccgtaatga cggcccagcc gacgctgttt gtgccgatag cgagcccaat ggagtacttc
      301 ttgtcggatc caccgccacc gaagttgatc tctccattgt tgaacttccg ccgcacctcc
      361 tccagtgtaa gggtgccggc tttgatcatc tcgcctccaa tcaggagctc ctccactgac
      421 aacacagctc cgttgcagtt tgtgatgtga ttgagtctag tgagctgagc tttataattg
      481 cccttaaagt ggccggagac aaacaggaac ttgaactcag tcacactact tgggtacact
      541 ttccaccatt cattggggtt gatgtgcttg ttgcgcgtct ggttctcttc gacataccgc
      601 tgcatctcgt cggcttgtcc tatgggcaag ttgtacccgc ctgagtaagc tttggtatcc
      661 acgatcaccc cataatcgat aggagatccg acagtataga tggcgccatc gggctttctg
      721 ctaccgccaa ggtgctttcc tctataaccg tagaccttca taaagaactc catgactttc
      781 atctcaagga tcctatcctg ggtactgttt ctggcgatct cgataagctc gatgtattcg
      841 tgtggcacgt acttcaactt gtgtctcaac tctgattttt tctcctcgag ttcagacttc
      901 acaagttggc tgctcacctt ccgcttcttc ttaggcatcg aaaatccggg tcgaaagtta
      961 cggttatcgc gcactctact ttccacaaat cctcacccaa a
//
