LOCUS       sequence-104065-         414 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway vector for generating yellow fluorescent protein fusions in 
            fungi.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 414)
  AUTHORS   Gong X, Hurtado O, Wang B, Yi M, Giraldo M, Wu C, Valent B, Goodin 
            MM, Farman M
  TITLE     pFPL vectors for high-throughput protein localization in fungi: 
            detecting cytoplasmic accumulation of putative effector proteins.
  JOURNAL   Mol Plant Microbe Interact. 2014 Nov 12.
  PUBMED    25390188
REFERENCE   2  (bases 1 to 414)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Plant 
            Microbe Interact. 2014 Nov 12."
COMMENT     Sequence Label: pFPL-Yg
FEATURES             Location/Qualifiers
     source          1..414
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(89..95,96..113,114..119))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(243..267)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 tcaacggtgg tatatccagt gatttttttc tccattttaa cttccttagc tcctgaaaat
       61 ctcgacggat cctaactcaa aatccacaca ttatacgagc cggaagcata aagtgtaaaa
      121 cctggggtgc ctaatgcggc cgccatagtg actggatatg ttgtgtttta cagtattatg
      181 tagtctgttt ttatgcaaaa tctaatttaa tatattgata tttatatcat tttacgtttc
      241 tcgttcagct tttttgtaca aacttgtgat ccaaatgtct atcgttcgta agcttatgaa
      301 tcactagtga attcgcggcc gcctgcatgt cgaccatatg ggagagctct gtaccgtgac
      361 cggagactct ttctggcatg cggagagacg gacggacgca gagagaaggg ctga
//
