LOCUS       sequence-104078-         931 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of PGC-1a with myc and his tags.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 931)
  AUTHORS   Ichida M, Nemoto S, Finkel T
  TITLE     Identification of a specific molecular repressor of the peroxisome 
            proliferator-activated receptor gamma Coactivator-1 alpha 
            (PGC-1alpha).
  JOURNAL   J Biol Chem. 2002 Dec 27. 277(52):50991-5.
  PUBMED    12397057
REFERENCE   2  (bases 1 to 931)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2002 Dec 27. 277(52):50991-5."
COMMENT     Sequence Label: pcDNA4 myc PGC-1 alpha
FEATURES             Location/Qualifiers
     source          1..931
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             363..392
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             408..425
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(448..465)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    454..678
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(811..830)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 tcagacctga cacaacacgg acagaactga gggaccgttt tgaagttttt ggtgaaattg
       61 aggagtgcac agtaaatctg cgggatgatg gagacagcta tggtttcatt acctaccgtt
      121 atacctgtga tgcttttgct gctcttgaaa atggatacac tttgcgcagg tcaaacgaaa
      181 ctgactttga gctgtacttt tgtggacgca agcaattttt caagtctaac tatgcagacc
      241 tagattcaaa ctcagatgac tttgaccctg cttccaccaa gagcaagtat gactctctgg
      301 attttgatag tttactgaaa gaagctcaga gaagcttgcg caggtctcga ggtcacccat
      361 tcgaacaaaa actcatctca gaagaggatc tgaatatgca taccggtcat catcaccatc
      421 accattgagt ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg
      481 ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt
      541 cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg
      601 gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg
      661 atgcggtggg ctctatggct tctgaggcgg aaagaaccag ctggggctct agggggtatc
      721 cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
      781 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
      841 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
      901 ttagtgcttt acggcacctc gaccccaaaa a
//
