LOCUS       sequence-10409-e        8404 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8404)
  AUTHORS   Prakriya M, Feske S, Gwack Y, Srikanth S, Rao A, Hogan PG
  TITLE     Orai1 is an essential pore subunit of the CRAC channel.
  JOURNAL   Nature. 2006 Sep 14. 443(7108):230-3.
  PUBMED    16921383
REFERENCE   2  (bases 1 to 8404)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2006 Sep 14. 443(7108):230-3."
COMMENT     Sequence Label: Orai1-myc E106Q MO70
FEATURES             Location/Qualifiers
     source          1..8404
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        6..385
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        386..589
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     540..560
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     misc_feature    829..1170
                     /label=MESV Psi
                     /note="packaging signal of murine embryonic stem cell 
                     virus"
     CDS             1237..1653
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1583..1605
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1621..1637
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     regulatory      1974..1983
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2579..2608
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2647..2670
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    2731..3192
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2787..2804)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3014..3033
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     regulatory      3227..3236
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3233..3235,3236..3238,3239..3952)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3233..3235,3236..3238,3239..3949)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3278..3299)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3539..3558)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3886..3907
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     promoter        3972..4471
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(4007..4030)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     4404..4423
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             4492..5091
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4492..4511)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4988..5008
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             5191..5705
                     /label=3' LTR
                     /note="3' long terminal repeat from murine embryonic stem 
                     cell virus"
     primer_bind     complement(5874..5890)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5874..5890)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5887..5909)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5898..5914
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5922..5928,5929..5946,5947..5952))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5967..5988
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6105..6122)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6276..6864)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6356..6375)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7035..7826,7827..7895))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7658..7677
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7896..8000)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8068..8086
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8124..8146)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     8246..8265
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gcgttgacat tgattattga ctagttatta atagtaatca attacggggt cattagttca
       61 tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc
      121 gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat
      181 agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt
      241 acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc
      301 cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta
      361 cgtattagtc atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg
      421 atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt
      481 gttttggcac caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac
      541 gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctc aataaaagag
      601 cccacaaccc ctcactcggc gcgccagtcc tccgatagac tgcgtcgccc gggtacccgt
      661 attcccaata aagcctcttg ctgtttgcat ccgaatcgtg gactcgctga tccttgggag
      721 ggtctcctca gattgattga ctgcccacct cgggggtctt tcatttggag gttccaccga
      781 gatttggaga cccctgccca gggaccaccg acccccccgc cgggaggtaa gctggccagc
      841 ggtcgtttcg tgtctgtctc tgtctttgtg cgtgtttgtg ccggcatcta atgtttgcgc
      901 ctgcgtctgt actagttagc taactagctc tgtatctggc ggacccgtgg tggaactgac
      961 gagttctgaa cacccggccg caaccctggg agacgtccca gggactttgg gggccgtttt
     1021 tgtggcccga cctgaggaag ggagtcgatg tggaatccga ccccgtcagg atatgtggtt
     1081 ctggtaggag acgagaacct aaaacagttc ccgcctccgt ctgaattttt gctttcggtt
     1141 tggaaccgaa gccgcgcgtc ttgtctgctg cagcgctgca gcatcgttct gtgttgtctc
     1201 tgtctgactg tgtttctgta tttgtctgaa aattagggcc agactgttac cactccctta
     1261 agtttgacct taggtcactg gaaagatgtc gagcggatcg ctcacaacca gtcggtagat
     1321 gtcaagaaga gacgttgggt taccttctgc tctgcagaat ggccaacctt taacgtcgga
     1381 tggccgcgag acggcacctt taaccgagac ctcatcaccc aggttaagat caaggtcttt
     1441 tcacctggcc cgcatggaca cccagaccag gtcccctaca tcgtgacctg ggaagccttg
     1501 gcttttgacc cccctccctg ggtcaagccc tttgtacacc ctaagcctcc gcctcctctt
     1561 cctccatccg ccccgtctct cccccttgaa cctcctcgtt cgaccccgcc tcgatcctcc
     1621 ctttatccag ccctcactcc ttctctaggc gccggaatta gatcgatctc tcgagatgca
     1681 tccggagccc gccccgcccc cgagccgtag cagtcccgag cttcccccaa gcggcggcag
     1741 caccaccagc ggcagccgcc ggagccgccg ccgcagcggg gacggggagc ccccgggggc
     1801 cccgccaccg ccgccgtccg ccgtcaccta cccggactgg atcggccaga gttactccga
     1861 ggtgatgagc ctcaacgagc actccatgca ggcgctgtcc tggcgcaagc tctacttgag
     1921 ccgcgccaag cttaaagcct ccagccggac ctcggctctg ctctccggct tcgccatggt
     1981 ggcaatggtg caggtgcagc tggacgctga ccacgactac ccaccggggc tgctcatcgc
     2041 cttcagtgcc tgcaccacag tgctggtggc tgtgcacctg tttgcgctca tgatcagcac
     2101 ctgcatcctg cccaacatcg aggcggtgag caacgtgcac aatctcaact cggtcaagga
     2161 gtccccccat gagcgcatgc accgccacat cgagctggcc tgggccttct ccaccgtcat
     2221 tggcacgctg ctcttcctag ctgaggtggt gctgctctgc tgggtcaagt tcttgcccct
     2281 caagaagcag ccaggccagc caaggcccac cagcaagccc cccgccagtg gcgcagcagc
     2341 caacgtcagc accagcggca tcaccccggg ccaggcagct gccatcgcct cgaccaccat
     2401 catggtgccc ttcggcctga tctttatcgt cttcgccgtc cacttctacc gctcactggt
     2461 tagccataag accgaccgac agttccagga gctcaacgag ctggcggagt ttgcccgctt
     2521 acaggaccag ctggaccaca gaggggacca ccccctgacg cccggcagcc actatgccga
     2581 gcaaaagctc atttctgagg aagatctcta gaattctacg ttaaccagat cgatattgcg
     2641 gccgctgact acaaggacga cgatgacaag tgagaattgt cgcgagtaat tccggttatt
     2701 ttccaccata ttgccgtctt ttggcaatgt gagggcccgg aaacctggcc ctgtcttctt
     2761 gacgagcatt cctaggggtc tttcccctct cgccaaagga atgcaaggtc tgttgaatgt
     2821 cgtgaaggaa gcagttcctc tggaagcttc ttgaagacaa acaacgtctg tagcgaccct
     2881 ttgcaggcag cggaaccccc cacctggcga caggtgcctc tgcggccaaa agccacgtgt
     2941 ataagataca cctgcaaagg cggcacaacc ccagtgccac gttgtgagtt ggatagttgt
     3001 ggaaagagtc aaatggctct cctcaagcgt attcaacaag gggctgaagg atgcccagaa
     3061 ggtaccccat tgtatgggat ctgatctggg gcctcggtgc acatgcttta catgtgttta
     3121 gtcgaggtta aaaaacgtct aggccccccg aaccacgggg acgtggtttt cctttgaaaa
     3181 acacgatgat aataccatgg ccaccaccca atcggatcca ccggtcgcca ccatggtgag
     3241 caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt
     3301 aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct
     3361 gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac
     3421 caccctgacc tacggcgtgc agtgcttcag ccgctacccc gaccacatga agcagcacga
     3481 cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga
     3541 cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg
     3601 catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga
     3661 gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga acggcatcaa
     3721 ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta
     3781 ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag
     3841 cacccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga
     3901 gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagt aaagcggccg
     3961 ttaattctac cgggtagggg aggcgctttt cccaaggcag tctggagcat gcgctttagc
     4021 agccccgctg ggcacttggc gctacacaag tggcctctgg cctcgcacac attccacatc
     4081 caccggtagg cgccaaccgg ctccgttctt tggtggcccc ttcgcgccac cttctactcc
     4141 tcccctagtc aggaagttcc cccccgcccc gcagctcgcg tcgtgcagga cgtgacaaat
     4201 ggaagtagca cgtctcacta gtctcgtgca gatggacagc accgctgagc aatggaagcg
     4261 ggtaggcctt tggggcagcg gccaatagca gctttgctcc ttcgctttct gggctcagag
     4321 gctgggaagg ggtgggtccg ggggcgggct caggggcggg ctcaggggcg gggcgggcgc
     4381 ccgaaggtcc tccggaggcc cggcattctg cacgcttcaa aagcgcacgt ctgccgcgct
     4441 gttctcctct tcctcatctc cgggcctttc gacctgcagc ccaagcttac catgaccgag
     4501 tacaagccca cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt acgcaccctc
     4561 gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgatccgga ccgccacatc
     4621 gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga catcggcaag
     4681 gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa
     4741 gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg ttcccggctg
     4801 gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg
     4861 ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg cagcgccgtc
     4921 gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct ggagacctcc
     4981 gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag
     5041 gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg acgcccgccc
     5101 cacgacccgc agcgcccgac cgaaaggagc gcacgacccc atgcatcgat aaaataaaag
     5161 attttattta gtctccagaa aaagggggga atgaaagacc ccacctgtag gtttggcaag
     5221 ctagcttaag taacgccatt ttgcaaggca tggaaaatac ataactgaga atagagaagt
     5281 tcagatcaag gttaggaaca gagagacagc agaatatggg ccaaacagga tatctgtggt
     5341 aagcagttcc tgccccggct cagggccaag aacagatggt ccccagatgc ggtcccgccc
     5401 tcagcagttt ctagagaacc atcagatgtt tccagggtgc cccaaggacc tgaaatgacc
     5461 ctgtgcctta tttgaactaa ccaatcagtt cgcttctcgc ttctgttcgc gcgcttctgc
     5521 tccccgagct caataaaaga gcccacaacc cctcactcgg cgcgccagtc ctccgataga
     5581 ctgcgtcgcc cgggtacccg tgtatccaat aaaccctctt gcagttgcat ccgacttgtg
     5641 gtctcgctgt tccttgggag ggtctcctct gagtgattga ctacccgtca gcgggggtct
     5701 ttcatgggta acagtttctt gaagttggag aacaacattc tgagggtagg agtcgaatat
     5761 taagtaatcc tgactcaatt agccactgtt ttgaatccac atactccaat actcctgaaa
     5821 tagttcatta tggacagcgc agaagagctg gggagaatta attcgtaatc atggtcatag
     5881 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
     5941 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
     6001 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
     6061 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
     6121 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     6181 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     6241 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     6301 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     6361 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     6421 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     6481 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     6541 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     6601 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     6661 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca
     6721 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     6781 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     6841 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     6901 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     6961 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     7021 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     7081 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     7141 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
     7201 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     7261 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     7321 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
     7381 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     7441 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     7501 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     7561 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     7621 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
     7681 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     7741 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     7801 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     7861 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
     7921 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     7981 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc
     8041 attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg
     8101 cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct
     8161 tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc
     8221 gggtgtcggg gctggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat
     8281 atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag gcgccattcg
     8341 ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc
     8401 cagc
//
