LOCUS       sequence-104091-        9293 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bacterial expression of MBP-MEG-3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9293)
  AUTHORS   Wang JT, Smith J, Chen BC, Schmidt H, Rasoloson D, Paix A, Lambrus 
            BG, Calidas D, Betzig E, Seydoux G
  TITLE     Regulation of RNA granule dynamics by phosphorylation of 
            serine-rich, intrinsically disordered proteins in C. elegans.
  JOURNAL   Elife. 2014 Dec 23;4. doi: 10.7554/eLife.04591.
  PUBMED    25535836
REFERENCE   2  (bases 1 to 9293)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2014-12-23"; volume: "4. doi"; pages: " 10.7554/eLife.04591"
COMMENT     Sequence Label: pJW4.23
FEATURES             Location/Qualifiers
     source          1..9293
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        3..80
                     /gene="lacI (mutant)"
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the 
                     promoter boosts expression of the lacI gene about 10-fold."
     CDS             81..1163
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     complement(100..119)
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        join(1406..1411,1412..1427,1428..1434)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    1442..1458
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1447..1469
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     CDS             1528..2628
                     /codon_start=1
                     /gene="malE (mutated)"
                     /product="maltose binding protein from E. coli"
                     /label=MBP
                     /note="This version of the gene does not encode a signal 
                     sequence, so MBP will remain in the cytosol."
                     /translation="MKIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLE
                     EKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKL
                     IAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIA
                     ADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGE
                     TAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFL
                     ENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAF
                     WYAVRTAVINAASGRQTVDEALKDAQT"
     primer_bind     2602..2625
                     /label=MBP-F
                     /note="Maltose binding protein, forward primer"
     CDS             2677..2688
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    2692..2716
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5309..5333)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(5384..5401)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5384..5400)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5393..5415)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(5579..5596)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      5749..5835
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      5927..5954
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        5973..6064
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6065..6133,6134..6925)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6283..6302)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      complement(6967..7480)
                     /direction=LEFT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7162..7183)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7374..7393
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     rep_origin      7591..8179
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8080..8099
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8333..8350
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    8365..8507
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(8433..8452)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8593..8615
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(8609..8800)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
ORIGIN
        1 ccgacaccat cgaatggtgc aaaacctttc gcggtatggc atgatagcgc ccggaagaga
       61 gtcaattcag ggtggtgaat gtgaaaccag taacgttata cgatgtcgca gagtatgccg
      121 gtgtctctta tcagaccgtt tcccgcgtgg tgaaccaggc cagccacgtt tctgcgaaaa
      181 cgcgggaaaa agtggaagcg gcgatggcgg agctgaatta cattcccaac cgcgtggcac
      241 aacaactggc gggcaaacag tcgttgctga ttggcgttgc cacctccagt ctggccctgc
      301 acgcgccgtc gcaaattgtc gcggcgatta aatctcgcgc cgatcaactg ggtgccagcg
      361 tggtggtgtc gatggtagaa cgaagcggcg tcgaagcctg taaagcggcg gtgcacaatc
      421 ttctcgcgca acgcgtcagt gggctgatca ttaactatcc gctggatgac caggatgcca
      481 ttgctgtgga agctgcctgc actaatgttc cggcgttatt tcttgatgtc tctgaccaga
      541 cacccatcaa cagtattatt ttctcccatg aagacggtac gcgactgggc gtggagcatc
      601 tggtcgcatt gggtcaccag caaatcgcgc tgttagcggg cccattaagt tctgtctcgg
      661 cgcgtctgcg tctggctggc tggcataaat atctcactcg caatcaaatt cagccgatag
      721 cggaacggga aggcgactgg agtgccatgt ccggttttca acaaaccatg caaatgctga
      781 atgagggcat cgttcccact gcgatgctgg ttgccaacga tcagatggcg ctgggcgcaa
      841 tgcgcgccat taccgagtcc gggctgcgcg ttggtgcgga tatctcggta gtgggatacg
      901 acgataccga agacagctca tgttatatcc cgccgttaac caccatcaaa caggattttc
      961 gcctgctggg gcaaaccagc gtggaccgct tgctgcaact ctctcagggc caggcggtga
     1021 agggcaatca gctgttgccc gtctcactgg tgaaaagaaa aaccaccctg gcgcccaata
     1081 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     1141 cccgactgga aagcgggcag tgagcgcaac gcaattaatg taagttagct cactcattag
     1201 gcacaattct catgtttgac agcttatcat cgactgcacg gtgcaccaat gcttctggcg
     1261 tcaggcagcc atcggaagct gtggtatggc tgtgcaggtc gtaaatcact gcataattcg
     1321 tgtcgctcaa ggcgcactcc cgttctggat aatgtttttt gcgccgacat cataacggtt
     1381 ctggcaaata ttctgaaatg agctgttgac aattaatcat cggctcgtat aatgtgtgga
     1441 attgtgagcg gataacaatt tcacacagga aacagccagt ccgtttaggt gttttcacga
     1501 gcacttcacc aacaaggacc atagcatatg aaaatcgaag aaggtaaact ggtaatctgg
     1561 attaacggcg ataaaggcta taacggtctc gctgaagtcg gtaagaaatt cgagaaagat
     1621 accggaatta aagtcaccgt tgagcatccg gataaactgg aagagaaatt cccacaggtt
     1681 gcggcaactg gcgatggccc tgacattatc ttctgggcac acgaccgctt tggtggctac
     1741 gctcaatctg gcctgttggc tgaaatcacc ccggacaaag cgttccagga caagctgtat
     1801 ccgtttacct gggatgccgt acgttacaac ggcaagctga ttgcttaccc gatcgctgtt
     1861 gaagcgttat cgctgattta taacaaagat ctgctgccga acccgccaaa aacctgggaa
     1921 gagatcccgg cgctggataa agaactgaaa gcgaaaggta agagcgcgct gatgttcaac
     1981 ctgcaagaac cgtacttcac ctggccgctg attgctgctg acgggggtta tgcgttcaag
     2041 tatgaaaacg gcaagtacga cattaaagac gtgggcgtgg ataacgctgg cgcgaaagcg
     2101 ggtctgacct tcctggttga cctgattaaa aacaaacaca tgaatgcaga caccgattac
     2161 tccatcgcag aagctgcctt taataaaggc gaaacagcga tgaccatcaa cggcccgtgg
     2221 gcatggtcca acatcgacac cagcaaagtg aattatggtg taacggtact gccgaccttc
     2281 aagggtcaac catccaaacc gttcgttggc gtgctgagcg caggtattaa cgccgccagt
     2341 ccgaacaaag agctggcaaa agagttcctc gaaaactatc tgctgactga tgaaggtctg
     2401 gaagcggtta ataaagacaa accgctgggt gccgtagcgc tgaagtctta cgaggaagag
     2461 ttggcgaaag atccacgtat tgccgccact atggaaaacg cccagaaagg tgaaatcatg
     2521 ccgaacatcc cgcagatgtc cgctttctgg tatgccgtgc gtactgcggt gatcaacgcc
     2581 gccagcggtc gtcagactgt cgatgaagcc ctgaaagacg cgcagactaa ttcgagctcg
     2641 aacaacaaca acaataacaa taacaacaac ctcgggatcg agggaaggat cacaagtttg
     2701 tacaaaaaag caggcttaat gagttcctca aaaccttacc caagcggtct gccaaactcg
     2761 agaagaaaaa gaggaggtcg ccgctcaagt agtcgtagca atcaggaaag tgcatccaac
     2821 aacatggaac accaaatcac tcttgacgaa ttgttcaatc cgatcgcaaa acaagacagt
     2881 gctcagtcaa caagccgtga atatggtgca aaatcgggga tatctcatca cggatccgta
     2941 tcattcaacg ggaatacatt catgaatggc caacagctga atcactcgat gactcgacat
     3001 ggtcgagtat tcaatcagtc gatgcatgca gctcaaggaa acggcagcaa cgcctttaac
     3061 agtattcctc ctacggcccc cgtcttctct gcagacttcc gccgcaactt gcaaacacgc
     3121 aatagctcca gctggtatga acgaagattc ccagtatcca cagatcagga cgacgttcag
     3181 caatcaaaca caagacgtag tagaagtcgt cagaatggac aacatggcct aagcttctcg
     3241 gatggttcga acaattatgg tcacgctggg aataagtcat tcagtgtgag ttccgttccc
     3301 gttgggttcc agaagcaaga aaataattct aagaaactgc gacaaaccaa tgttcaccag
     3361 caatgtcttg gaaataaaag cttcaacgct caagctggag ttcatggaca cgcttttaaa
     3421 aaaggccata aggacaataa aaatgcatct ggaaaggagg ttatcaattc tagtcttgtc
     3481 caaaagcacg atgctattaa gtcgcggaat ttgaaccaga gcttctctgg atttcctaca
     3541 catgaaacct catcgatgaa aaatcaacaa cagaaatcaa gaaacgacag aaaaaaatca
     3601 cgtggtagca gcaacttcca ggatcgtact tacttcaaca caaatgacga tgaattaact
     3661 gacgatgtgt tcatagacga ttccatggat gctgcccgtg gtcggagatc tcgatcagtc
     3721 actaagaaac ttcaacaatc gacttattca aagcagaatg caggcagtaa acaattgact
     3781 gaaaaatgca aatcttctga agaagcggct aaacgtaacc tggtatccaa tgtattcagc
     3841 aaggacggca ctgaactctc cattgaacag ttattggaaa ttgtcagcat gaagatcgga
     3901 caacagattc acttgccttc cagctctcat ggagaatgca gcaatttgaa tcggacatta
     3961 ccagcaagtg atctaaattg ctcaattggt gaagactttg actccagttt tgtggatgct
     4021 aacaaccaaa ccctaccagt gagtcttccc aagaaaactt cactgtcgat caagcgccgt
     4081 ggaagttctc gtagtgcctc ccgtctggcc agtcttgatg tgacactgga aactgttgaa
     4141 gaagacgagg aacccacgcc aagtccacag ccgagttctc ctccaaagat ctcaagaaga
     4201 aagtggacag gaacttttga tgccaacgtt gaggaaatga gacgtttact tcatggggac
     4261 cctgagatgc cgaaatctgc aaatcgtgct tcctcaagca aggatcagat caatcggaac
     4321 aatgtggatg taaagagaac accttcctcg tcaatcatcc caactccaaa ggctttgatc
     4381 ggagaaagat gtctgacatc atccagcaag tcttcaaagt tgaacaagtc acttggcgtg
     4441 gttgattcta aagctacaaa atcaccaatg tattccgtca cagtttctgg aaaagaaaca
     4501 gcttcaggaa agcgaattgc tcaaaagttg actccaaagg ttgtggcgct ggaaagctct
     4561 tacattaccg gaattccagt ttccacggat tgtaatggtt gcccaactcc gaagagaagt
     4621 ggaatcaatt gtgaaattcg tgcagctgaa gtatacaatc aagctgggaa atggcccttc
     4681 gaaatcacca gtgatccggc tccacttcct tgtgaatcag ccgatcggat cgaatatcct
     4741 tctcaagatt gcactcaaga tccagcttca acctcgccac cacctcgcat ttctgaaagc
     4801 ttgacagcat tcctggaagc tcagcaagac tttaacgact acattgatac taactacaaa
     4861 gaaaagacac aactcctgaa agtgaatttg aatattcacg ggatgtcacc tgaaagatgg
     4921 ctgtacttga attatttttg caccgagaca attccaagat tggatggtcc ttatgccgat
     4981 gatccaagag ttccacctgt gcgaaatatg ttcagaaaat ggttcctccg ttttgctgaa
     5041 gcctgcttag gaaatcctca ccagcttgca gttatgcaag agattgctgc cacgtttgtt
     5101 caggctcggc tggacgatac gagcagctcc acagactcca ccaatatgct ctacatgttg
     5161 tggaaggagt gtatcggaca gaagaatatc atcgcaattg ctgatgcgtg tttacttgca
     5221 catcttcgga aatcggatcc tatcaaatac ttgaatgtaa aacgggattg gctggaatct
     5281 atttttgacc cacccagaga tcaatgagac ccagctttct tgtacaaagt ggtgatattt
     5341 cagaattcgg atcctctaga gtcgacctgc aggcaagctt ggcactggcc gtcgttttac
     5401 aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc
     5461 ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc
     5521 gcagcctgaa tggcgaatgg cagcttggct gttttggcgg atgagataag attttcagcc
     5581 tgatacagat taaatcagaa cgcagaagcg gtctgataaa acagaatttg cctggcggca
     5641 gtagcgcggt ggtcccacct gaccccatgc cgaactcaga agtgaaacgc cgtagcgccg
     5701 atggtagtgt ggggtctccc catgcgagag tagggaactg ccaggcatca aataaaacga
     5761 aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc
     5821 ctgagtagga caaatccgcc gggagcggat ttgaacgttg cgaagcaacg gcccggaggg
     5881 tggcgggcag gacgcccgcc ataaactgcc aggcatcaaa ttaagcagaa ggccatcctg
     5941 acggatggcc tttttgcgtt tctacaaact cttttgttta tttttctaaa tacattcaaa
     6001 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     6061 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
     6121 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
     6181 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
     6241 ccccgaagaa cgtttcccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
     6301 atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
     6361 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
     6421 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
     6481 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
     6541 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
     6601 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
     6661 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
     6721 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
     6781 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
     6841 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
     6901 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
     6961 tgatttaccc cggttgataa tcagaaaagc cccaaaaaca ggaagattgt ataagcaaat
     7021 atttaaattg taaacgttaa tattttgtta aaattcgcgt taaatttttg ttaaatcagc
     7081 tcatttttta accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc
     7141 gagatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac
     7201 tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca
     7261 cccaaatcaa gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg
     7321 agcccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag
     7381 aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc
     7441 accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt aaaaggatct aggtgaagat
     7501 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     7561 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     7621 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     7681 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct
     7741 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     7801 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     7861 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     7921 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     7981 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     8041 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     8101 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     8161 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     8221 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
     8281 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
     8341 agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg
     8401 tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta
     8461 agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc
     8521 caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag
     8581 ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg
     8641 cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgcag cgattcacag atgtctgcct
     8701 gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa
     8761 agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg
     8821 gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg
     8881 ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat
     8941 ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag
     9001 atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg
     9061 tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc
     9121 atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta
     9181 tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg
     9241 acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgaa att
//
