LOCUS       sequence-104098-        8528 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bacterial expression of MBP-PPTR-2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8528)
  AUTHORS   Wang JT, Smith J, Chen BC, Schmidt H, Rasoloson D, Paix A, Lambrus 
            BG, Calidas D, Betzig E, Seydoux G
  TITLE     Regulation of RNA granule dynamics by phosphorylation of 
            serine-rich, intrinsically disordered proteins in C. elegans.
  JOURNAL   Elife. 2014 Dec 23;4. doi: 10.7554/eLife.04591.
  PUBMED    25535836
REFERENCE   2  (bases 1 to 8528)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2014-12-23"; volume: "4. doi"; pages: " 10.7554/eLife.04591"
COMMENT     Sequence Label: pJW4.13
FEATURES             Location/Qualifiers
     source          1..8528
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        3..80
                     /gene="lacI (mutant)"
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the 
                     promoter boosts expression of the lacI gene about 10-fold."
     CDS             81..1163
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     complement(100..119)
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        join(1406..1411,1412..1427,1428..1434)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    1442..1458
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1447..1469
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     CDS             1528..2628
                     /codon_start=1
                     /gene="malE (mutated)"
                     /product="maltose binding protein from E. coli"
                     /label=MBP
                     /note="This version of the gene does not encode a signal 
                     sequence, so MBP will remain in the cytosol."
                     /translation="MKIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLE
                     EKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKL
                     IAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIA
                     ADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGE
                     TAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFL
                     ENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAF
                     WYAVRTAVINAASGRQTVDEALKDAQT"
     primer_bind     2602..2625
                     /label=MBP-F
                     /note="Maltose binding protein, forward primer"
     CDS             2677..2688
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    2692..2716
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4544..4568)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(4619..4636)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4619..4635)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4628..4650)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(4814..4831)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      4984..5070
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      5162..5189
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        5208..5299
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5300..5368,5369..6160)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5518..5537)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      complement(6202..6715)
                     /direction=LEFT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6397..6418)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6609..6628
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     rep_origin      6826..7414
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7315..7334
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7568..7585
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    7600..7742
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(7668..7687)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7828..7850
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(7844..8035)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
ORIGIN
        1 ccgacaccat cgaatggtgc aaaacctttc gcggtatggc atgatagcgc ccggaagaga
       61 gtcaattcag ggtggtgaat gtgaaaccag taacgttata cgatgtcgca gagtatgccg
      121 gtgtctctta tcagaccgtt tcccgcgtgg tgaaccaggc cagccacgtt tctgcgaaaa
      181 cgcgggaaaa agtggaagcg gcgatggcgg agctgaatta cattcccaac cgcgtggcac
      241 aacaactggc gggcaaacag tcgttgctga ttggcgttgc cacctccagt ctggccctgc
      301 acgcgccgtc gcaaattgtc gcggcgatta aatctcgcgc cgatcaactg ggtgccagcg
      361 tggtggtgtc gatggtagaa cgaagcggcg tcgaagcctg taaagcggcg gtgcacaatc
      421 ttctcgcgca acgcgtcagt gggctgatca ttaactatcc gctggatgac caggatgcca
      481 ttgctgtgga agctgcctgc actaatgttc cggcgttatt tcttgatgtc tctgaccaga
      541 cacccatcaa cagtattatt ttctcccatg aagacggtac gcgactgggc gtggagcatc
      601 tggtcgcatt gggtcaccag caaatcgcgc tgttagcggg cccattaagt tctgtctcgg
      661 cgcgtctgcg tctggctggc tggcataaat atctcactcg caatcaaatt cagccgatag
      721 cggaacggga aggcgactgg agtgccatgt ccggttttca acaaaccatg caaatgctga
      781 atgagggcat cgttcccact gcgatgctgg ttgccaacga tcagatggcg ctgggcgcaa
      841 tgcgcgccat taccgagtcc gggctgcgcg ttggtgcgga tatctcggta gtgggatacg
      901 acgataccga agacagctca tgttatatcc cgccgttaac caccatcaaa caggattttc
      961 gcctgctggg gcaaaccagc gtggaccgct tgctgcaact ctctcagggc caggcggtga
     1021 agggcaatca gctgttgccc gtctcactgg tgaaaagaaa aaccaccctg gcgcccaata
     1081 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     1141 cccgactgga aagcgggcag tgagcgcaac gcaattaatg taagttagct cactcattag
     1201 gcacaattct catgtttgac agcttatcat cgactgcacg gtgcaccaat gcttctggcg
     1261 tcaggcagcc atcggaagct gtggtatggc tgtgcaggtc gtaaatcact gcataattcg
     1321 tgtcgctcaa ggcgcactcc cgttctggat aatgtttttt gcgccgacat cataacggtt
     1381 ctggcaaata ttctgaaatg agctgttgac aattaatcat cggctcgtat aatgtgtgga
     1441 attgtgagcg gataacaatt tcacacagga aacagccagt ccgtttaggt gttttcacga
     1501 gcacttcacc aacaaggacc atagcatatg aaaatcgaag aaggtaaact ggtaatctgg
     1561 attaacggcg ataaaggcta taacggtctc gctgaagtcg gtaagaaatt cgagaaagat
     1621 accggaatta aagtcaccgt tgagcatccg gataaactgg aagagaaatt cccacaggtt
     1681 gcggcaactg gcgatggccc tgacattatc ttctgggcac acgaccgctt tggtggctac
     1741 gctcaatctg gcctgttggc tgaaatcacc ccggacaaag cgttccagga caagctgtat
     1801 ccgtttacct gggatgccgt acgttacaac ggcaagctga ttgcttaccc gatcgctgtt
     1861 gaagcgttat cgctgattta taacaaagat ctgctgccga acccgccaaa aacctgggaa
     1921 gagatcccgg cgctggataa agaactgaaa gcgaaaggta agagcgcgct gatgttcaac
     1981 ctgcaagaac cgtacttcac ctggccgctg attgctgctg acgggggtta tgcgttcaag
     2041 tatgaaaacg gcaagtacga cattaaagac gtgggcgtgg ataacgctgg cgcgaaagcg
     2101 ggtctgacct tcctggttga cctgattaaa aacaaacaca tgaatgcaga caccgattac
     2161 tccatcgcag aagctgcctt taataaaggc gaaacagcga tgaccatcaa cggcccgtgg
     2221 gcatggtcca acatcgacac cagcaaagtg aattatggtg taacggtact gccgaccttc
     2281 aagggtcaac catccaaacc gttcgttggc gtgctgagcg caggtattaa cgccgccagt
     2341 ccgaacaaag agctggcaaa agagttcctc gaaaactatc tgctgactga tgaaggtctg
     2401 gaagcggtta ataaagacaa accgctgggt gccgtagcgc tgaagtctta cgaggaagag
     2461 ttggcgaaag atccacgtat tgccgccact atggaaaacg cccagaaagg tgaaatcatg
     2521 ccgaacatcc cgcagatgtc cgctttctgg tatgccgtgc gtactgcggt gatcaacgcc
     2581 gccagcggtc gtcagactgt cgatgaagcc ctgaaagacg cgcagactaa ttcgagctcg
     2641 aacaacaaca acaataacaa taacaacaac ctcgggatcg agggaaggat cacaagtttg
     2701 tacaaaaaag caggcttaat gttgagatca aagaagaagg acaaggaaaa tgggaaatca
     2761 gagaaaaagg acaaagagaa ggataagaaa tcaatgaaag atgacggtgc tggaagcagt
     2821 aaggcatcta caattcctac gatccgaaca gaagatgttg gaggagatat gattcctgca
     2881 gatgcaccac caccaacaaa tattggccga acaaacacgt acggaggagg gccagtgata
     2941 ccgcgaagag agcgccgcca atcaagtagc atgttcaaca tttcacaaaa cagagagctg
     3001 caaagacttc cagctatcaa agatgcggat ccgagcgaga gagaaacact gttcattcaa
     3061 aaacttcgac aatgctgcgt tgtttttgac tttgccaatg atgctctcag cgatttaaaa
     3121 ttcaaagaag tcaaacgggc agctctaaat gaactcgtcg accacgtttc cggagctcca
     3181 aaaggatcgc tatctgatgc tgtctatcca gaagctatcg gcatgttctc cactaatctt
     3241 ttccgtcctt tgagcccacc gaccaatcca attggtgctg aatttgaccc cgatgaagac
     3301 gaaccaacat tagaagctgc ctggccacat ctgcagctcg tctacgagtt tttcctgaga
     3361 ttcctggagt gccccgattt tcaatctcaa gttgcaaaac gttacattga tcaaaatttc
     3421 attcttcgtc ttctcatgat tatggacagt gaagatccac gtgaacgtga ctttctgaag
     3481 acaactttac atcgaattta tggaaagttc ctcggacatc gtgcttacat tcggaagcaa
     3541 atcaacaaca ttttctactc gttcatctac gaaactgaga ggcacaacgg gattgctgaa
     3601 ttactcgaga ttctaggtag cattatcaat ggattcgctc ttcccttgaa agaggaacac
     3661 aaaactttct tgctccgagt tcttcttccg ctacacaagg tcaaatcatt atctgtatat
     3721 catcctcaac tcgcgtactg tgtcgtacag ttcattgaaa aagactcatc actgacagag
     3781 cctgtaatca gtgggatgct acgcttctgg ccaaaacaac atagtcccaa ggaagtgatg
     3841 ttcctgaatg aattggaaga agtcctcgat gtgatagagc cgaatgagtt ccaaaaaatc
     3901 atgactccat tgttctcaca aattgctcgc tgtgtcagca gtccacactt ccaagttgcc
     3961 gaacgagctc tctactattg gaataatgaa tatgtgatgt cattagtagc ggacaatgct
     4021 cgtgtgataa ttccaataat gttccctgtt cttttcaaaa acagcaagtc acattggaat
     4081 aaaacgattc atggacttat ctacaacgcc ctcaagatgt tcatggaaat gaatcaaaaa
     4141 ctgtttgacg agtgttccca agcttatcag aaagatcgag ttcaagaaaa gacattgaat
     4201 gaagaaaaag aacgcatctg gaacaatatt gagaaacagg caatgggaaa cccgcaatat
     4261 gttgaagtta aagcactatt tgctcgattt aatccagatg aaatcatcag ctcgcgtcag
     4321 cagaatggag ttgacgagaa tatgaagact tcaactgtgc ttagcaagga tgaaattctg
     4381 aaaaatgctg taggagtgag ctcgatgaaa aacgatatgg atttcggacc caaccacaaa
     4441 caatccgatt tccctccaga tgagcaaacg acgagagctt taggcgagta caagcgtcac
     4501 gatccatttt tgaagaaagt gactagcacc gacgaacagt gagacccagc tttcttgtac
     4561 aaagtggtga tatttcagaa ttcggatcct ctagagtcga cctgcaggca agcttggcac
     4621 tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc
     4681 ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc
     4741 cttcccaaca gttgcgcagc ctgaatggcg aatggcagct tggctgtttt ggcggatgag
     4801 ataagatttt cagcctgata cagattaaat cagaacgcag aagcggtctg ataaaacaga
     4861 atttgcctgg cggcagtagc gcggtggtcc cacctgaccc catgccgaac tcagaagtga
     4921 aacgccgtag cgccgatggt agtgtggggt ctccccatgc gagagtaggg aactgccagg
     4981 catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg
     5041 tcggtgaacg ctctcctgag taggacaaat ccgccgggag cggatttgaa cgttgcgaag
     5101 caacggcccg gagggtggcg ggcaggacgc ccgccataaa ctgccaggca tcaaattaag
     5161 cagaaggcca tcctgacgga tggccttttt gcgtttctac aaactctttt gtttattttt
     5221 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
     5281 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
     5341 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
     5401 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
     5461 ccttgagagt tttcgccccg aagaacgttt cccaatgatg agcactttta aagttctgct
     5521 atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca
     5581 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
     5641 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
     5701 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
     5761 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
     5821 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg
     5881 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
     5941 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
     6001 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
     6061 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
     6121 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
     6181 atatatactt tagattgatt taccccggtt gataatcaga aaagccccaa aaacaggaag
     6241 attgtataag caaatattta aattgtaaac gttaatattt tgttaaaatt cgcgttaaat
     6301 ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat cccttataaa
     6361 tcaaaagaat agaccgagat agggttgagt gttgttccag tttggaacaa gagtccacta
     6421 ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca
     6481 ctacgtgaac catcacccaa atcaagtttt ttggggtcga ggtgccgtaa agcactaaat
     6541 cggaacccta aagggagccc ccgatttaga gcttgacggg gaaagccggc gaacgtggcg
     6601 agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc
     6661 acgctgcgcg taaccaccac acccgccgcg cttaatgcgc cgctacaggg cgcgtaaaag
     6721 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
     6781 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     6841 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     6901 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     6961 accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     7021 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     7081 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     7141 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     7201 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     7261 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     7321 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     7381 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     7441 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
     7501 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
     7561 cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct
     7621 tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct
     7681 gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg
     7741 cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat
     7801 ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt
     7861 catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgcagcgatt
     7921 cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg
     7981 tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg
     8041 cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga
     8101 tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta
     8161 aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc
     8221 gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga
     8281 tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga
     8341 aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc
     8401 acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta
     8461 gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc
     8521 ccgaaatt
//
