LOCUS       sequence-104100-        8896 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bacterial expression of MBP-PGL-1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8896)
  AUTHORS   Wang JT, Smith J, Chen BC, Schmidt H, Rasoloson D, Paix A, Lambrus 
            BG, Calidas D, Betzig E, Seydoux G
  TITLE     Regulation of RNA granule dynamics by phosphorylation of 
            serine-rich, intrinsically disordered proteins in C. elegans.
  JOURNAL   Elife. 2014 Dec 23;4. doi: 10.7554/eLife.04591.
  PUBMED    25535836
REFERENCE   2  (bases 1 to 8896)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2014-12-23"; volume: "4. doi"; pages: " 10.7554/eLife.04591"
COMMENT     Sequence Label: pJW4.02
FEATURES             Location/Qualifiers
     source          1..8896
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        3..80
                     /gene="lacI (mutant)"
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the 
                     promoter boosts expression of the lacI gene about 10-fold."
     CDS             81..1163
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     complement(100..119)
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        join(1406..1411,1412..1427,1428..1434)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    1442..1458
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1447..1469
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     CDS             1528..2628
                     /codon_start=1
                     /gene="malE (mutated)"
                     /product="maltose binding protein from E. coli"
                     /label=MBP
                     /note="This version of the gene does not encode a signal 
                     sequence, so MBP will remain in the cytosol."
                     /translation="MKIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLE
                     EKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKL
                     IAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIA
                     ADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGE
                     TAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFL
                     ENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAF
                     WYAVRTAVINAASGRQTVDEALKDAQT"
     primer_bind     2602..2625
                     /label=MBP-F
                     /note="Maltose binding protein, forward primer"
     CDS             2677..2688
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    2692..2716
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4912..4936)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(4987..5004)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4987..5003)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4996..5018)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(5182..5199)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      5352..5438
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      5530..5557
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        5576..5667
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5668..5736,5737..6528)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5886..5905)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      complement(6570..7083)
                     /direction=LEFT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6765..6786)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6977..6996
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     rep_origin      7194..7782
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7683..7702
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7936..7953
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    7968..8110
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(8036..8055)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8196..8218
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(8212..8403)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
ORIGIN
        1 ccgacaccat cgaatggtgc aaaacctttc gcggtatggc atgatagcgc ccggaagaga
       61 gtcaattcag ggtggtgaat gtgaaaccag taacgttata cgatgtcgca gagtatgccg
      121 gtgtctctta tcagaccgtt tcccgcgtgg tgaaccaggc cagccacgtt tctgcgaaaa
      181 cgcgggaaaa agtggaagcg gcgatggcgg agctgaatta cattcccaac cgcgtggcac
      241 aacaactggc gggcaaacag tcgttgctga ttggcgttgc cacctccagt ctggccctgc
      301 acgcgccgtc gcaaattgtc gcggcgatta aatctcgcgc cgatcaactg ggtgccagcg
      361 tggtggtgtc gatggtagaa cgaagcggcg tcgaagcctg taaagcggcg gtgcacaatc
      421 ttctcgcgca acgcgtcagt gggctgatca ttaactatcc gctggatgac caggatgcca
      481 ttgctgtgga agctgcctgc actaatgttc cggcgttatt tcttgatgtc tctgaccaga
      541 cacccatcaa cagtattatt ttctcccatg aagacggtac gcgactgggc gtggagcatc
      601 tggtcgcatt gggtcaccag caaatcgcgc tgttagcggg cccattaagt tctgtctcgg
      661 cgcgtctgcg tctggctggc tggcataaat atctcactcg caatcaaatt cagccgatag
      721 cggaacggga aggcgactgg agtgccatgt ccggttttca acaaaccatg caaatgctga
      781 atgagggcat cgttcccact gcgatgctgg ttgccaacga tcagatggcg ctgggcgcaa
      841 tgcgcgccat taccgagtcc gggctgcgcg ttggtgcgga tatctcggta gtgggatacg
      901 acgataccga agacagctca tgttatatcc cgccgttaac caccatcaaa caggattttc
      961 gcctgctggg gcaaaccagc gtggaccgct tgctgcaact ctctcagggc caggcggtga
     1021 agggcaatca gctgttgccc gtctcactgg tgaaaagaaa aaccaccctg gcgcccaata
     1081 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     1141 cccgactgga aagcgggcag tgagcgcaac gcaattaatg taagttagct cactcattag
     1201 gcacaattct catgtttgac agcttatcat cgactgcacg gtgcaccaat gcttctggcg
     1261 tcaggcagcc atcggaagct gtggtatggc tgtgcaggtc gtaaatcact gcataattcg
     1321 tgtcgctcaa ggcgcactcc cgttctggat aatgtttttt gcgccgacat cataacggtt
     1381 ctggcaaata ttctgaaatg agctgttgac aattaatcat cggctcgtat aatgtgtgga
     1441 attgtgagcg gataacaatt tcacacagga aacagccagt ccgtttaggt gttttcacga
     1501 gcacttcacc aacaaggacc atagcatatg aaaatcgaag aaggtaaact ggtaatctgg
     1561 attaacggcg ataaaggcta taacggtctc gctgaagtcg gtaagaaatt cgagaaagat
     1621 accggaatta aagtcaccgt tgagcatccg gataaactgg aagagaaatt cccacaggtt
     1681 gcggcaactg gcgatggccc tgacattatc ttctgggcac acgaccgctt tggtggctac
     1741 gctcaatctg gcctgttggc tgaaatcacc ccggacaaag cgttccagga caagctgtat
     1801 ccgtttacct gggatgccgt acgttacaac ggcaagctga ttgcttaccc gatcgctgtt
     1861 gaagcgttat cgctgattta taacaaagat ctgctgccga acccgccaaa aacctgggaa
     1921 gagatcccgg cgctggataa agaactgaaa gcgaaaggta agagcgcgct gatgttcaac
     1981 ctgcaagaac cgtacttcac ctggccgctg attgctgctg acgggggtta tgcgttcaag
     2041 tatgaaaacg gcaagtacga cattaaagac gtgggcgtgg ataacgctgg cgcgaaagcg
     2101 ggtctgacct tcctggttga cctgattaaa aacaaacaca tgaatgcaga caccgattac
     2161 tccatcgcag aagctgcctt taataaaggc gaaacagcga tgaccatcaa cggcccgtgg
     2221 gcatggtcca acatcgacac cagcaaagtg aattatggtg taacggtact gccgaccttc
     2281 aagggtcaac catccaaacc gttcgttggc gtgctgagcg caggtattaa cgccgccagt
     2341 ccgaacaaag agctggcaaa agagttcctc gaaaactatc tgctgactga tgaaggtctg
     2401 gaagcggtta ataaagacaa accgctgggt gccgtagcgc tgaagtctta cgaggaagag
     2461 ttggcgaaag atccacgtat tgccgccact atggaaaacg cccagaaagg tgaaatcatg
     2521 ccgaacatcc cgcagatgtc cgctttctgg tatgccgtgc gtactgcggt gatcaacgcc
     2581 gccagcggtc gtcagactgt cgatgaagcc ctgaaagacg cgcagactaa ttcgagctcg
     2641 aacaacaaca acaataacaa taacaacaac ctcgggatcg agggaaggat cacaagtttg
     2701 tacaaaaaag caggcttaat ggaggctaac aagcgagaaa ttgtggattt cggtgggctg
     2761 agatcctact ttttcccaaa tttggcccac tacatcacga agaatgacga agaactgttc
     2821 aacaatacat ctcaggccaa taagttggct gctttcgtgt tgggagcttc caaagacgct
     2881 cctggagacg aagacatttt ggagatgatc ttgccgaacg acgctaacgc tgccgttatt
     2941 gctgctggca tggacgtttg tcttcttctc ggagataaat ttcgtccgaa atttgatgct
     3001 gctgctgaga aattgtcagg attgggacat gctcatgatc tggtttctgt catcgatgac
     3061 gacaagaagc tcggaatgct cgcccgtaag gccaagctga agaagactga agacgctaag
     3121 attcttcaag ctcttctcaa agtaatcgca atcgatgatg ccgccgaaaa gtttgttgag
     3181 ctcacggaac ttgtcagcca actggatctg gactttgatg tttatgtcct aactaaaatc
     3241 ctcggactta tctctgagga gactagcgac gaggtcgaca ttatccgtga taacgtcgtc
     3301 aacgcatttg atagctgcaa gccgttgttg aaacagttga tgttggatgg tccgaaatct
     3361 gaacctgccg atccattcat tagtctcctc atggatccac tcgaagagtc tgttgggaaa
     3421 gttgtgaacc atatcgctca acttttcgag gaagcgagta aaaatgaagg agacgagtca
     3481 cttgtcttga gatctcaact cggatatcag ctctttttcc ttatcgttcg ttcacttgca
     3541 gatggaaagc gcgaggtttc caagaaaatc ctctctggga ttcctacgag tgtcagagcg
     3601 gaagtctttc caggattgca aagaagtgtc tacaagagtg cggtgttcct cggcaatcac
     3661 atcatccaag ttcttttagg atctaagaag tcttttgagg attgggatgt tgttggagtc
     3721 gcgaaagact tggagagcgc ctggaaaaga cgagccattg cggaacttat caagaagttc
     3781 caagtctcaa ttctagagca atgcttcgat aagcccgttc cactgattcc tcagagtcca
     3841 ctaaacaatg atgcggtgat cgataatgtg aataaagctc tacagtttgc actgtggctt
     3901 acagagtttt acggatccga gaatgagact gaagcgctgg gcgagcttcg tttcttggat
     3961 tccacttcaa aaaatcttct cgtcgatagc ttcaagaaat ttgttcaagg aatcaactcg
     4021 aagactcatg tcactcgaat tgttgaatca ctggagaagt gttgtttgtc cgatacacca
     4081 agtggcagaa agagcaatgt gcaaccatcc acttcccaac agcaggattc cgcctacacg
     4141 aaagaggaaa tgacgacggt tcacaacaca tacagcgtaa atactaaagc tcaagttctc
     4201 aacggtctca gtgataccaa ttcttccggg ttacttgtcg attcaaagga ttcactcagt
     4261 cttcaggaga tttcttgtga cgaagtggat tcttcgactt tgctaagctc atcaagaaac
     4321 attggagaag gtgttactgt aaaagcggtc gatcctgtcc cagaaaaggt taacgatgca
     4381 caacaacagc aaacagttaa cgagatcgag atggcttcgg atgccaatca agatacttct
     4441 tcaagtgctt cgccagaagt agcgccaagt ttttccaccg atggctggga ttctccaacg
     4501 aaatcagtag ctctgccacc gggcatgcag cagatagatg aagaggaaac cacagttgct
     4561 gacaaagatt ctacaccaca accacaagct cgtgctgaaa ctgcatgggg gtcaggcgac
     4621 gctactccaa tgcctcttcc tgccccgact aatcaataca aagtcagtgg atttggggaa
     4681 gccaaagttg caaaaggatt cggtcaattt gctcccacca gttcagctta tggaggagga
     4741 ggtggtcgcg gaggatatgg cggtggagac cgtggaggca gaggtggata cggcggagat
     4801 cgtggaggta gaggtggata cggcggggga gaccgcggag gacgaggtgg atacggcgga
     4861 gatcgtggac gtggtggtta cgggggtcgt ggtggacgcg gaggtttcta aacccagctt
     4921 tcttgtacaa agtggtgata tttcagaatt cggatcctct agagtcgacc tgcaggcaag
     4981 cttggcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact
     5041 taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac
     5101 cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tggcagcttg gctgttttgg
     5161 cggatgagat aagattttca gcctgataca gattaaatca gaacgcagaa gcggtctgat
     5221 aaaacagaat ttgcctggcg gcagtagcgc ggtggtccca cctgacccca tgccgaactc
     5281 agaagtgaaa cgccgtagcg ccgatggtag tgtggggtct ccccatgcga gagtagggaa
     5341 ctgccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct
     5401 gttgtttgtc ggtgaacgct ctcctgagta ggacaaatcc gccgggagcg gatttgaacg
     5461 ttgcgaagca acggcccgga gggtggcggg caggacgccc gccataaact gccaggcatc
     5521 aaattaagca gaaggccatc ctgacggatg gcctttttgc gtttctacaa actcttttgt
     5581 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     5641 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
     5701 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
     5761 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
     5821 ggtaagatcc ttgagagttt tcgccccgaa gaacgtttcc caatgatgag cacttttaaa
     5881 gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc
     5941 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
     6001 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
     6061 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
     6121 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
     6181 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta
     6241 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
     6301 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
     6361 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
     6421 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
     6481 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
     6541 gtttactcat atatacttta gattgattta ccccggttga taatcagaaa agccccaaaa
     6601 acaggaagat tgtataagca aatatttaaa ttgtaaacgt taatattttg ttaaaattcg
     6661 cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc
     6721 cttataaatc aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga
     6781 gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg
     6841 atggcccact acgtgaacca tcacccaaat caagtttttt ggggtcgagg tgccgtaaag
     6901 cactaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga
     6961 acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg
     7021 tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg
     7081 cgtaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
     7141 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     7201 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     7261 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     7321 gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac
     7381 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     7441 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     7501 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     7561 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     7621 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     7681 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     7741 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     7801 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     7861 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
     7921 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat
     7981 tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa
     8041 tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt
     8101 catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct
     8161 cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt
     8221 ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg
     8281 cagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag
     8341 cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt
     8401 cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg
     8461 agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg
     8521 tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca
     8581 atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc
     8641 gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga
     8701 aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca
     8761 gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg
     8821 ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc
     8881 aacgctgccc gaaatt
//
