LOCUS       sequence-104103-       11797 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Singlet donor (phase 2) for in vivo RMCE with MiMIC inserts to make 
            Gal4 driver..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11797)
  AUTHORS   Diao F, Ironfield H, Luan H, Diao F, Shropshire WC, Ewer J, Marr E, 
            Potter CJ, Landgraf M, White BH
  TITLE     Plug-and-play genetic access to drosophila cell types using 
            exchangeable exon cassettes.
  JOURNAL   Cell Rep. 2015 Mar 3;10(8):1410-21. doi: 
            10.1016/j.celrep.2015.01.059. Epub 2015 Feb 26.
  PUBMED    25732830
REFERENCE   2  (bases 1 to 11797)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2015.01.059"; journalName: "Cell Rep"; date: 
            "2015-03-3- 3"; volume: "10"; issue: "8"; pages: "1410-21"
COMMENT     Sequence Label: pC-(loxP2-attB2-SA(2)-T2A-Gal4-Hsp70)
FEATURES             Location/Qualifiers
     source          1..11797
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(235..823)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(315..334)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(994..1785,1786..1854))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1617..1636
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1855..1959)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2027..2045
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2083..2105)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2205..2224
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     misc_feature    join(2761..2791,2792..2993)
                     /label=P element 3' end
                     /note="P element 3' end"
     primer_bind     3015..3035
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        3017..3035
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     protein_bind    3069..3102
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    3218..3287
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     CDS             3455..3508
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             3509..6154
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4"
                     /product="GAL4 transcriptional activator"
                     /label=GAL4
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVSIDSAAHHDNSTIPLDF
                     MPRDALHGFDWSEEDDMSDGLPFLKTDPNNNGFFGDGSLLCILRSIGFKPENYTNSNVN
                     RLPTMITDRYTLASRSTTSRLLQSYLNNFHPYCPIVHSPTLMMLYNNQIEIASKDQWQI
                     LFNCILAIGAWCIEGESTDIDVFYYQNAKSHLTSKVFESGSIILVTALHLLSRYTQWRQ
                     KTNTSYNFHSFSIRMAISLGLNRDLPSSFSDSSILEQRRRIWWSVYSWEIQLSLLYGRS
                     IQLSQNTISFPSSVDDVQRTTTGPTIYHGIIETARLLQVFTKIYELDKTVTAEKSPICA
                     KKCLMICNEIEEVSRQAPKFLQMDISTTALTNLLKEHPWLSFTRFELKWKQLSLIIYVL
                     RDFFTNFTQKKSQLEQDQNDHQSYEVKRCSIMLSDAAQRTVMSVSSYMDNHNVTPYFAW
                     NCSYYLFNAVLVPIKTLLSNSKSNAENNETAQLLQQINTVLMLLKKLATFKIQTCEKYI
                     QVLEEVCAPFLLSQCAIPLPHISYNNSNGSAIKNIVGSATIAQYPTLPEENVNNISVKY
                     VSPGSVGPSPVPLKSGASFSDLVKLLSNRPPSRNSPVTIPRSTPSHRSVTPFLGQQQQL
                     QSLVPLTPSALFGGANFNQSGNIADSSLSFTFTNSSNGPNLITTQTNSQALSQPIASSN
                     VHDNFMNNEITASKIDDGNNSKPLSPGWTDQTAYNAFGITTGMFNTTTMDDVYNYLFDD
                     EDTPPNPKKE"
     protein_bind    6832..6901
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     protein_bind    7017..7050
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     gene            7073..11201
                     /gene="white"
                     /label=mini-white
                     /note="This modified version of the white gene lacks part 
                     of the first intron."
     primer_bind     complement(7082..7100)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(7552..7623,8018..8291,8366..9020,9082..9397,9618..9749,
                     9820..10434)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLLPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     misc_feature    join(11212..11766,11767..11797)
                     /label=P element 5' end
ORIGIN
        1 ggccagaccc acgtagtcca gcggcagatc ggcggcggag aagttaagcg tctccaggat
       61 gaccttgccc gaactggggc acgtggtgtt cgacgatgtg cagctaattt cgcccggctc
      121 cacgtccgcc cattggttaa tcagcagacc ctcgttggcg taacggaacc atgagaggta
      181 cgacaaccat ttgaggtata ctggcaccga gcccgagttc aagaagaagg cgtttttcca
      241 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
      301 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
      361 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
      421 gctttctcaa tgctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
      481 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
      541 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
      601 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
      661 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
      721 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
      781 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
      841 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
      901 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
      961 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     1021 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     1081 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     1141 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     1201 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     1261 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     1321 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     1381 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     1441 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     1501 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     1561 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa cacgggataa
     1621 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     1681 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     1741 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     1801 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     1861 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     1921 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     1981 acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac
     2041 gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct
     2101 cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg
     2161 cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat
     2221 tgtactgaga gtgcaccata tgcggtgtga aataccgcac cgaatcgcgc ggaactaacg
     2281 acagtcgctc caaggtcgtc gaacaaaagg tgaatgtgtt gcggagagcg ggtgggagac
     2341 agcgaaagag caactacgaa acgtggtgtg gtggaggtga attatgaaga gggcgcgcga
     2401 tttgaaaagt atgtatataa aaaatatatc ccggtgtttt atgtagcgat aaacgagttt
     2461 ttgatgtaag gtatgcaggt gtgtaagtct tttggttaga agacaaatcc aaagtctact
     2521 tgtggggatg ttcgaagggg aaatacttgt attctatagg tcatatcttg tttttattgg
     2581 cacaaatata attacattag ctttttgagg gggcaataaa cagtaaacac gatggtaata
     2641 atggtaaaaa aaaaaacaag cagttatttc ggatatatgt cggctactcc ttgcgtcggg
     2701 cccgaagtct tagagccaga tatgcgagca cccggaagct cacgatgaga atggccagac
     2761 catgatgaaa taacataagg tggtcccgtc ggcaagagac atccacttaa cgtatgcttg
     2821 caataagtgc gagtgaaagg aatagtattc tgagtgtcgt attgagtctg agtgagacag
     2881 cgatatgatt gttgattaac ccttagcatg tccgtggggt ttgaattaac tcataatatt
     2941 aattagacga aattattttt aaagttttat ttttaataat ttgcgagtac gcaaagcttg
     3001 gctgcatcca acgcgcaatt aaccctcact aaagggaaca aaagctgggt accgggcccc
     3061 ccctcgagat aacttcgtat aatgtatgct atacgaagtt atcaattggc ttattgggat
     3121 ttcaatttaa gatgctgcta aataaagtta gtcacttacg gactggggag gcaccccgtc
     3181 atcactcgcc agaaatgcaa ttgatgtagg tcacggtctc gaagccgcgg tgcgggtgcc
     3241 agggcgtgcc cttgggctcc ccgggcgcgt actccacctc acccatctgg tccatcatga
     3301 tggtcgactc tagaagtcga tccaacatgg cgacttgtcc catccccggc atgtttaaat
     3361 atactaatta ttcttgaact aattttaatc aaccgattta tctctcttcc gcaggtcggg
     3421 aggttccggt ggaagcggag gtagcggcgg atccgagggc cgcggcagcc tgctgacctg
     3481 cggcgatgtg gaggagaacc ccgggcccat gaagctgctg agcagcatcg agcaggcctg
     3541 cgatatctgc cgcctgaaga agctgaagtg cagcaaggag aagcccaagt gcgccaagtg
     3601 cctgaagaac aactgggagt gccgctacag ccccaagacc aagcgcagcc ccctgacccg
     3661 cgcccacctg accgaggtgg agagccgcct ggagcgcctg gagcagctgt tcctgctgat
     3721 cttcccccgc gaggatctgg atatgatcct gaagatggat agcctgcagg atatcaaggc
     3781 cctgctgacc ggcctgttcg tgcaggataa cgtgaacaag gatgccgtga ccgatcgcct
     3841 ggccagcgtg gagaccgata tgcccctgac cctgcgccag caccgcatca gcgccaccag
     3901 cagcagcgag gagagcagca acaagggcca gcgccagctg accgtgagca tcgatagcgc
     3961 cgcccaccac gataacagca ccatccccct ggatttcatg ccccgcgatg ccctgcacgg
     4021 cttcgattgg agcgaggagg atgatatgag cgatggcctg cccttcctga agaccgatcc
     4081 caacaacaac ggcttcttcg gcgatggcag cctgctgtgc atcctgcgca gcatcggctt
     4141 caagcccgag aactacacca acagcaacgt gaaccgcctg cccaccatga tcaccgatcg
     4201 ctacaccctg gccagccgca gcaccaccag ccgcctgctg cagagctacc tgaacaactt
     4261 ccacccctac tgccccatcg tgcacagccc caccctgatg atgctgtaca acaaccagat
     4321 cgagatcgcc agcaaggatc agtggcagat cctgttcaac tgcatcctgg ccatcggcgc
     4381 ctggtgcatc gagggcgaga gcaccgatat cgatgtgttc tactaccaga acgccaagag
     4441 ccacctgacc agcaaggtgt tcgagagcgg cagcatcatc ctggtgaccg ccctgcacct
     4501 gctgagccgc tacacccagt ggcgccagaa gaccaacacc agctacaact tccacagctt
     4561 cagcatccgc atggccatca gcctgggcct gaaccgcgat ctgcccagca gcttcagcga
     4621 tagcagcatc ctggagcagc gccgccgcat ctggtggagc gtgtacagct gggagatcca
     4681 gctgagcctg ctgtacggcc gcagcatcca gctgagccag aacaccatca gcttccccag
     4741 cagcgtggat gatgtgcagc gcaccaccac cggccccacc atctaccacg gcatcatcga
     4801 gaccgcccgc ctgctgcagg tgttcaccaa gatctacgag ctggataaga ccgtgaccgc
     4861 cgagaagagc cccatctgcg ccaagaagtg cctgatgatc tgcaacgaga tcgaggaggt
     4921 gagccgccag gcccccaagt tcctgcagat ggatatcagc accaccgccc tgaccaacct
     4981 gctgaaggag cacccctggc tgagcttcac ccgcttcgag ctgaagtgga agcagctgag
     5041 cctgatcatc tacgtgctgc gcgatttctt caccaacttc acccagaaga agagccagct
     5101 ggagcaggat cagaacgatc accagagcta cgaggtgaag cgctgcagca tcatgctgag
     5161 cgatgccgcc cagcgcaccg tgatgagcgt gagcagctac atggataacc acaacgtgac
     5221 cccctacttc gcctggaact gcagctacta cctgttcaac gccgtgctgg tgcccatcaa
     5281 gaccctgctg agcaacagca agagcaacgc cgagaacaac gagaccgccc agctgctgca
     5341 gcagatcaac accgtgctga tgctgctgaa gaagctggcc accttcaaga tccagacctg
     5401 cgagaagtac atccaggtgc tggaggaggt gtgcgccccc ttcctgctga gccagtgcgc
     5461 catccccctg ccccacatca gctacaacaa cagcaacggc agcgccatca agaacatcgt
     5521 gggcagcgcc accatcgccc agtaccccac cctgcccgag gagaacgtga acaacatcag
     5581 cgtgaagtac gtgagccccg gcagcgtggg acccagcccc gtgcccctga agagcggcgc
     5641 cagcttcagc gatctggtga agctgctgag caaccgcccc cccagccgca acagccccgt
     5701 gaccatcccc cgcagcaccc ccagccaccg cagcgtgacc cccttcctgg gccagcagca
     5761 gcagctgcag agcctggtgc ccctgacccc cagcgccctg ttcggcggcg ccaacttcaa
     5821 ccagagcggc aacatcgccg atagcagcct gagcttcacc ttcaccaaca gcagcaacgg
     5881 ccccaacctg atcaccaccc agaccaacag ccaggccctg agccagccca tcgccagcag
     5941 caacgtgcac gataacttca tgaacaacga gatcaccgcc agcaagatcg atgatggcaa
     6001 caacagcaag cccctgagcc ccggctggac cgatcagacc gcctacaacg ccttcggcat
     6061 caccaccggc atgttcaaca ccaccaccat ggatgatgtg tacaactacc tgttcgatga
     6121 tgaggatacc ccccccaacc ccaagaagga gtaaaatgaa tcgtagatac tgaaaaaccc
     6181 cgcaagttca cttcaactgt gcatcgtgca ccatctcaat ttctttcatt tatacatcgt
     6241 tttgccttct tttatgtaac tatactcctc taagtttcaa tcttggccat gtaacctctg
     6301 atctatagaa ttttttaaat gactagaatt aatgcccatc ttttttttgg acctaaattc
     6361 ttcatgaaaa tatattacga gggcttattc agaagcttat cgataccgtc gactaaagcc
     6421 aaatagaaat tattcagttc tggcttaagt ttttaaaagt gatattattt atttggttgt
     6481 aaccaaccaa aagaatgtaa ataactaata cataattatg ttagttttaa gttagcaaca
     6541 aattgatttt agctatatta gctacttggt taataaatag aatatattta tttaaagata
     6601 attcgttttt attgtcaggg agtgagtttg cttaaaaact cgtttggatc cggaggtagc
     6661 ggtggaagcg gaggttccgg caggtaagtt attgaacaat ggcatcaaat gccttcatca
     6721 tcactaccct ttagccctta agaccccaca atgaccttac ccactcagag aaaaaagtaa
     6781 atatgaaagc ccatttgaac ttctaagctt gtcgaccatc atgatggacc agatgggtga
     6841 ggtggagtac gcgcccgggg agcccaaggg cacgccctgg cacccgcacc gcggcttcga
     6901 gaccgtgacc tacatcatac aataaatatt cacaccccta aaaaacgaag ctgcatttgc
     6961 aaattgcata caattatgcc agagtaaaaa ttataattgt tattatttaa atttgaataa
     7021 cttcgtataa tgtatgctat acgaagttat agatccacta gaaggcctaa ttctagtatg
     7081 tatgtaagtt aataaaaccc atttttgcgg aaagtagata aaaaaaacat tttttttttt
     7141 tactgcactg gatatcattg aacttatctg atcagtttta aatttacttc gatccaaggg
     7201 tatttgatgt accaggttct ttcgattacc tctcactcaa aatgacattc cactcaaagt
     7261 cagcgctgtt tgcctccttc tctgtccaca gaaatatcgc cgtctctttc gccgctgcgt
     7321 ccgctatctc tttcgccacc gtttgtagcg ttacctagcg tcaatgtccg ccttcagttg
     7381 cactttgtca gcggtttcgt gacgaagctc caagcggttt acgccatcaa ttaaacacaa
     7441 agtgctgtgc caaaactcct ctcgcttctt atttttgttt gttttttgag tgattggggt
     7501 ggtgattggt tttgggtggg taagcagggg aaagtgtgaa aaatcccggc aatgggccaa
     7561 gaggatcagg agctattaat tcgcggaggc agcaaacacc catctgccga gcatctgaac
     7621 aatgtgagta gtacatgtgc atacatctta agttcacttg atctatagga actgcgattg
     7681 caacatcaaa ttgtctgcgg cgtgagaact gcgacccaca aaaatcccaa accgcaatcg
     7741 cacaaacaaa tagtgacacg aaacagatta ttctggtagc tgtgctcgct atataagaca
     7801 atttttaaga tcatatcatg atcaagacat ctaaaggcat tcattttcga ctacattctt
     7861 ttttacaaaa aatataacaa ccagatattt taagctgatc ctagatgcac aaaaaataaa
     7921 taaaagtata aacctacttc gtaggatact tcgttttgtt cggggttaga tgagcataac
     7981 gcttgtagtt gatatttgag atcccctatc attgcagggt gacagcggag cggcttcgca
     8041 gagctgcatt aaccagggct tcgggcaggc caaaaactac ggcacgctcc tgccacccag
     8101 tccgccggag gactccggtt cagggagcgg ccaactagcc gagaacctca cctatgcctg
     8161 gcacaatatg gacatctttg gggcggtcaa tcagccgggc tccggatggc ggcagctggt
     8221 caaccggaca cgcggactat tctgcaacga gcgacacata ccggcgccca ggaaacattt
     8281 gctcaagaac ggtgagtttc tattcgcagt cggctgatct gtgtgaaatc ttaataaagg
     8341 gtccaattac caatttgaaa ctcagtttgc ggcgtggcct atccgggcga acttttggcc
     8401 gtgatgggca gttccggtgc cggaaagacg accctgctga atgcccttgc ctttcgatcg
     8461 ccgcagggca tccaagtatc gccatccggg atgcgactgc tcaatggcca acctgtggac
     8521 gccaaggaga tgcaggccag gtgcgcctat gtccagcagg atgacctctt tatcggctcc
     8581 ctaacggcca gggaacacct gattttccag gccatggtgc ggatgccacg acatctgacc
     8641 tatcggcagc gagtggcccg cgtggatcag gtgatccagg agctttcgct cagcaaatgt
     8701 cagcacacga tcatcggtgt gcccggcagg gtgaaaggtc tgtccggcgg agaaaggaag
     8761 cgtctggcat tcgcctccga ggcactaacc gatccgccgc ttctgatctg cgatgagccc
     8821 acctccggac tggactcatt taccgcccac agcgtcgtcc aggtgctgaa gaagctgtcg
     8881 cagaagggca agaccgtcat cctgaccatt catcagccgt cttccgagct gtttgagctc
     8941 tttgacaaga tccttctgat ggccgagggc agggtagctt tcttgggcac tcccagcgaa
     9001 gccgtcgact tcttttccta gtgagttcga tgtgtttatt aagggtatct agcattacat
     9061 tacatctcaa ctcctatcca gcgtgggtgc ccagtgtcct accaactaca atccggcgga
     9121 cttttacgta caggtgttgg ccgttgtgcc cggacgggag atcgagtccc gtgatcggat
     9181 cgccaagata tgcgacaatt ttgctattag caaagtagcc cgggatatgg agcagttgtt
     9241 ggccaccaaa aatttggaga agccactgga gcagccggag aatgggtaca cctacaaggc
     9301 cacctggttc atgcagttcc gggcggtcct gtggcgatcc tggctgtcgg tgctcaagga
     9361 accactcctc gtaaaagtgc gacttattca gacaacggtg agtggttcca gtggaaacaa
     9421 atgatataac gcttacaatt cttggaaaca aattcgctag attttagtta gaattgcctg
     9481 attccacacc cttcttagtt tttttcaatg agatgtatag tttatagttt tgcagaaaat
     9541 aaataaattt catttaactc gcgaacatgt tgaagatatg aatattaatg agatgcgagt
     9601 aacattttaa tttgcagatg gttgccatct tgattggcct catctttttg ggccaacaac
     9661 tcacgcaagt gggcgtgatg aatatcaacg gagccatctt cctcttcctg accaacatga
     9721 cctttcaaaa cgtctttgcc acgataaatg taagtcttgt ttagaataca tttgcatatt
     9781 aataatttac taactttcta atgaatcgat tcgatttagg tgttcacctc agagctgcca
     9841 gtttttatga gggaggcccg aagtcgactt tatcgctgtg acacatactt tctgggcaaa
     9901 acgattgccg aattaccgct ttttctcaca gtgccactgg tcttcacggc gattgcctat
     9961 ccgatgatcg gactgcgggc cggagtgctg cacttcttca actgcctggc gctggtcact
    10021 ctggtggcca atgtgtcaac gtccttcgga tatctaatat cctgcgccag ctcctcgacc
    10081 tcgatggcgc tgtctgtggg tccgccggtt atcataccat tcctgctctt tggcggcttc
    10141 ttcttgaact cgggctcggt gccagtatac ctcaaatggt tgtcgtacct ctcatggttc
    10201 cgttacgcca acgagggtct gctgattaac caatgggcgg acgtggagcc gggcgaaatt
    10261 agctgcacat cgtcgaacac cacgtgcccc agttcgggca aggtcatcct ggagacgctt
    10321 aacttctccg ccgccgatct gccgctggac tacgtgggtc tggccattct catcgtgagc
    10381 ttccgggtgc tcgcatatct ggctctaaga cttcgggccc gacgcaagga gtagccgaca
    10441 tatatccgaa ataactgctt gttttttttt ttaccattat taccatcgtg tttactgttt
    10501 attgccccct caaaaagcta atgtaattat atttgtgcca ataaaaacaa gatatgacct
    10561 atagaataca agtatttccc cttcgaacat ccccacaagt agactttgga tttgtcttct
    10621 aaccaaaaga cttacacacc tgcatacctt acatcaaaaa ctcgtttatc gctacataaa
    10681 acaccgggat atatttttta tatacatact tttcaaatcg cgcgccctct tcataattca
    10741 cctccaccac accacgtttc gtagttgctc tttcgctgtc tcccacccgc tctccgcaac
    10801 acattcacct tttgttcgac gaccttggag cgactgtcgt tagttccgcg cgattcggtt
    10861 cgctcaaatg gttccgagtg gttcatttcg tctcaataga aattagtaat aaatatttgt
    10921 atgtacaatt tatttgctcc aatatatttg tatatatttc cctcacagct atatttattc
    10981 taatttaata ttatgacttt ttaaggtaat tttttgtgac ctgttcggag tgattagcgt
    11041 tacaatttga actgaaagtg acatccagtg tttgttcctt gtgtagatgc atctcaaaaa
    11101 aatggtgggc ataatagtgt tgtttatata tatcaaaaat aacaactata ataataagaa
    11161 tacatttaat ttagaaaatg cttggatttc actggaacta gaattaattc ggctgctgct
    11221 ctaaacgacg catttcgtac tccaaagtac gaattttttc cctcaagctc ttattttcat
    11281 taaacaatga acaggaccta acgcacagtc acgttattgt ttacataaat gatttttttt
    11341 actattcaaa cttactctgt ttgtgtactc ccactggtat agccttcttt tatcttttct
    11401 ggttcaggct ctatcacttt actaggtacg gcatctgcgt tgagtcgcct ccttttaaat
    11461 gtctgacctt ttgcaggtgc agccttccac tgcgaatcat taaagtgggt atcacaaatt
    11521 tgggagtttt caccaaggct gcacccaagg ctctgctccc acaattttct cttaatagca
    11581 cacttcggca cgtgaattaa ttttactcca gtcacagctt tgcagcaaaa tttgcaatat
    11641 ttcatttttt tttattccac gtaagggtta atgttttcaa aaaaaaattc gtccgcacac
    11701 aacctttcct ctcaacaagc aaacgtgcac tgaatttaag tgtatacttc ggtaagcttc
    11761 ggctatcgac gggaccacct tatgttattt catcatg
//
