LOCUS       sequence-104107-         848 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Recombinase-mediated cassette exchange in ES cells to insert the Dre
            recombinase gene at the mouse Pvalb gene stop codon.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 848)
  AUTHORS   Madisen L, Garner AR, Shimaoka D, Chuong AS, Klapoetke NC, Li L, van
            der Bourg A, Niino Y, Egolf L, Monetti C, Gu H, Mills M, Cheng A, 
            Tasic B, Nguyen TN, Sunkin SM, Benucci A, Nagy A, Miyawaki A, 
            Helmchen F, Empson RM, Knopfel T, Boyden ES, Reid RC, Carandini M, 
            Zeng H
  TITLE     Transgenic mice for intersectional targeting of neural sensors and 
            effectors with high specificity and performance.
  JOURNAL   Neuron. 2015 Mar 4;85(5):942-58. doi: 10.1016/j.neuron.2015.02.022.
  PUBMED    25741722
REFERENCE   2  (bases 1 to 848)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2015.02"; journalName: "Neuron"; date: "2015-03-4-
            4"; volume: "85"; issue: "5"; pages: "942-58"
COMMENT     Sequence Label: Pvalb-2A-Dre Flp-in replacement vector
FEATURES             Location/Qualifiers
     source          1..848
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             320..373
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             377..397
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 aatagtatag gaacttcttt cggccggcct cagggggttc tcagagacaa tgaatgacga
       61 gttctaagtc taccctgacc cctccggtca aacagcagct gttcaaagaa acggccaggc
      121 tcagagcctc cattccctca tccacagagc aggcatggtg actaacaaca cagtcgcagc
      181 tgaagcccaa ggagatattg tactcggagg gtttaagata gctggtgcac ggcagacgtg
      241 gggatgcttg ccgaagataa gagtgtgtca tttttctttt gcagaattct ccactctggt
      301 ggctgaaagc ggttccggag agggcagagg aagtcttcta acatgcggtg acgtggagga
      361 gaatcccggc cctatgccta agaagaagag gaaggtttct gagctgatta ttagtggttc
      421 atctggtgga ttcctgcgaa acatcggcaa agagtatcag gaggccgctg aaaacttcat
      481 gaggtttatg aatgaccagg gggcgtacgc tcctaacact ttgagggatt tgaggttggt
      541 ctttcatagc tgggccagat ggtgccatgc tcggcagctt gcatggtttc caattagtcc
      601 tgaaatggca cgcgaatact ttcttcagtt gcacgatgca gacctggcct ccactaccat
      661 cgacaagcac tatgctatgc ttaatatgct tctgtcccac tgcggactgc cacccttgtc
      721 cgacgacaag tcagtgagtc ttgccatgag aagaattaga agagaagccg caaccgaaaa
      781 gggtgagagg acaggacagg caatccccct gcgctgggac gacctgaagc tgctggatgt
      841 gctgctca
//
