LOCUS       sequence-104109-         877 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  QUAS driving GFP with beta crystalline CFP eye marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 877)
  AUTHORS   Subedi A, Macurak M, Gee ST, Monge E, Goll MG, Potter CJ, Parsons 
            MJ, Halpern ME
  TITLE     Adoption of the Q transcriptional regulatory system for zebrafish 
            transgenesis.
  JOURNAL   Methods. 2014 Apr 1;66(3):433-40. doi: 10.1016/j.ymeth.2013.06.012. 
            Epub 2013 Jun 20.
  PUBMED    23792917
REFERENCE   2  (bases 1 to 877)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ymeth.2013.06.012"; journalName: "Methods"; date: 
            "2014-04-1- 1"; volume: "66"; issue: "3"; pages: "433-40"
COMMENT     Sequence Label: QUAS:GFP;betacrystallin:CFP
FEATURES             Location/Qualifiers
     source          1..877
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    21..41
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     CDS             complement(join(70..783,784..786,787..789))
                     /codon_start=1
                     /product="enhanced CFP"
                     /label=ECFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(115..136)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     464..483
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     723..744
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      786..795
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 aacgacggcc agtgaattat caactatgta taataaagtt gtcgaggctg atcagcgggt
       61 ttggccgctt tacttgtaca gctcgtccat gccgagagtg atcccggcgg cggtcacgaa
      121 ctccagcagg accatgtgat cgcgcttctc gttggggtct ttgctcaggg cggactgggt
      181 gctcaggtag tggttgtcgg gcagcagcac ggggccgtcg ccgatggggg tgttctgctg
      241 gtagtggtcg gcgagctgca cgctgccgtc ctcgatgttg tggcggatct tgaagttggc
      301 cttgatgccg ttcttctgct tgtcggcggt gatatagacg ttgtggctga tgtagttgta
      361 ctccagcttg tgccccagga tgttgccgtc ctccttgaag tcgatgccct tcagctcgat
      421 gcggttcacc agggtgtcgc cctcgaactt cacctcggcg cgggtcttgt agttgccgtc
      481 gtccttgaag aagatggtgc gctcctggac gtagccttcg ggcatggcgg acttgaagaa
      541 gtcgtgctgc ttcatgtggt cggggtagcg gctgaagcac tgcacgcccc aggtcagggt
      601 ggtcacgagg gtgggccagg gcacgggcag cttgccggtg gtgcagatga acttcagggt
      661 cagcttgccg taggtggcat cgccctcgcc ctcgccggac acgctgaact tgtggccgtt
      721 tacgtcgccg tccagctcga ccaggatggg caccaccccg gtgaacagct cctcgccctt
      781 gctcaccatg gtggcgggat cccctagcaa aaatacagat actgcagttt ctgataggaa
      841 ggttgagcag aatagcaggg gaaggaaaca ggaggaa
//
