LOCUS       sequence-104111-         840 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  QUAS driving GFP with beta crystalline CFP eye marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 840)
  AUTHORS   Subedi A, Macurak M, Gee ST, Monge E, Goll MG, Potter CJ, Parsons 
            MJ, Halpern ME
  TITLE     Adoption of the Q transcriptional regulatory system for zebrafish 
            transgenesis.
  JOURNAL   Methods. 2014 Apr 1;66(3):433-40. doi: 10.1016/j.ymeth.2013.06.012. 
            Epub 2013 Jun 20.
  PUBMED    23792917
REFERENCE   2  (bases 1 to 840)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ymeth.2013.06.012"; journalName: "Methods"; date: 
            "2014-04-1- 1"; volume: "66"; issue: "3"; pages: "433-40"
COMMENT     Sequence Label: QUAS:GFP;betacrystallin:CFP
FEATURES             Location/Qualifiers
     source          1..840
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     89..105
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     89..105
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    125..145
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    336..360
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(366..368,369..371,372..830)
                     /codon_start=1
                     /product="improved N-terminal fragment of mVenus for use in
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN155(I152L)
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMA"
     primer_bind     complement(411..432)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(672..691)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 attagtcttg atagagaggc tgcaaatagc aggaaacgtg agcagagact ccctggtgtc
       61 tgaaacacag gccagatggg ccctcgagca ggaaacagct atgaccatga ttacgccaag
      121 ctatcaactt tgtatagaaa agttggggta atcgcttatc ctcggataaa caattatcct
      181 cacgggtaat cgcttatccg ctcgggtaat cgcttatcct cgggtaatcg cttatccttg
      241 caagggtcga ctctagaggg tatataatgg atcccatcgc gtctcagcct cactttgagc
      301 tcctccacac gaattccctc gacctcgaag acgcgtcaag tttgtacaaa aaagcaggct
      361 ggaccatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc
      421 tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca
      481 cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc
      541 ccaccctcgt gaccaccctg acctacggcg tgcagtgctt cagccgctac cccgaccaca
      601 tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca
      661 tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca
      721 ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg
      781 ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcatggcc gacaagcaga
//
