LOCUS       sequence-104117-         689 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  E. coli expression vector with N-terminal AviTag and a C-terminal 
            Hexa-HIS tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 689)
  TITLE     Arrowsmith Lab Plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 689)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: p28BIOH-LIC
FEATURES             Location/Qualifiers
     source          1..689
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             16..60
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     protein_bind    complement(109..142)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        157..602
                     /gene="sacR"
                     /label=sacB promoter
                     /note="sacB promoter and control region"
ORIGIN
        1 agatatacca tgagcggcct gaacgatatt tttgaagcgc agaaaattga atggcatgaa
       61 ggcagcgctg gaggttcagg tattatgagt tctcctcctg aaagatccat aacttcgtat
      121 agcatacatt atacgaagtt atgcggccgc gacgtccaca tatacctgcc gttcactatt
      181 atttagtgaa atgagatatt atgatatttt ctgaattgtg attaaaaagg caactttatg
      241 cccatgcaac agaaactata aaaaatacag agaatgaaaa gaaacagata gattttttag
      301 ttctttaggc ccgtagtctg caaatccttt tatgattttc tatcaaacaa aagaggaaaa
      361 tagaccagtt gcaatccaaa cgagagtcta atagaatgag gtcgaaaagt aaatcgcgcg
      421 ggtttgttac tgataaagca ggcaagacct aaaatgtgta aagggcaaag tgtatacttt
      481 ggcgtcaccc cttacatatt ttaggtcttt ttttattgtg cgtaactaac ttgccatctt
      541 caaacaggag ggctggaaga agcagaccgc taacacagta cataaaaaag gagacatgaa
      601 cgatgaacat caaaaagttt gcaaaacaag caacagtatt aacctttact accgcactgc
      661 tggcaggagg cgcaactcaa gcgtttgcg
//
