LOCUS       sequence-104121-        9522 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentivirus production of CheRiff channelrhodopsin actuator.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9522)
  AUTHORS   Hochbaum DR, Zhao Y, Farhi SL, Klapoetke N, Werley CA, Kapoor V, Zou
            P, Kralj JM, Maclaurin D, Smedemark-Margulies N, Saulnier JL, 
            Boulting GL, Straub C, Cho YK, Melkonian M, Wong GK, Harrison DJ, 
            Murthy VN, Sabatini BL, Boyden ES, Campbell RE, Cohen AE
  TITLE     All-optical electrophysiology in mammalian neurons using engineered 
            microbial rhodopsins.
  JOURNAL   Nat Methods. 2014 Aug;11(8):825-33. doi: 10.1038/nmeth.3000. Epub 
            2014 Jun 22.
  PUBMED    24952910
REFERENCE   2  (bases 1 to 9522)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.3000"; journalName: "Nat Methods"; date: "2014-08"; 
            volume: "11"; issue: "8"; pages: "825-33"
COMMENT     Sequence Label: pMOS010: pLX304-CheRiff-GFP
FEATURES             Location/Qualifiers
     source          1..9522
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..181
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    228..353
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    846..1079
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1264..1308
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1483..1983
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     1902..1920
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             1995..2390
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    2450..2567
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2684..2987
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2988..3191
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3142..3162
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    3214..3238
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      3270..3279
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3276..4223
                     /codon_start=1
                     /product="blue light-activated optogenetic actuator derived
                     from Scherffelia dubia channelrhodopsin (Hochbaum et al., 
                     2014)"
                     /label=CheRiff
                     /translation="MGGAPAPDAHSAPPGNDSAGGSEYHAPAGYQVNPPYHPVHGYEEQ
                     CSSIYIYYGALWEQETARGFQWFAVFLSALFLAFYGWHAYKASVGWEEVYVCSVELIKV
                     ILEIYFEFTSPAMLFLYGGNITPWLRYAEWLLTCPVILIHLSNITGLSEAYNKRTMALL
                     VSDLGTICMGVTAALATGWVKWLFYCIGLVYGTQTFYNAGIIYVESYYIMPAGGCKKLV
                     LAMTAVYYSSWLMFPGLFIFGPEGMHTLSVAGSTIGHTIADLLSKNIWGLLGHFLRIKI
                     HEHIIMYGDIRRPVSSQFLGRKVDVLAFVTEEDKV"
     CDS             join(4293..4295,4296..5009)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(4293..4295,4296..5006)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(4335..4356)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4596..4615)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4943..4964
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(5039..5063)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5065..5106
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    5148..5736
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5201..5221)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5619..5630)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5808..6041
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6119..6240
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6156..6175)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6210..6229
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6280..6415
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6435..6454)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6436..6454)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6464..6481)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6464..6480)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6473..6495)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6622..7077
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6709..6728)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6919..6940
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7103..7207
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7208..7276,7277..8068)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7426..7445)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8239..8827
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8728..8747
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8981..8998
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9115..9136
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9151..9156,9157..9174,9175..9181)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9189..9205
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9194..9216
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9213..9229
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9213..9229
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9248..9268
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9250..9268
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        9296..9522
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
ORIGIN
        1 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
       61 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
      121 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
      181 agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca
      241 ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc
      301 caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta
      361 agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa
      421 aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc
      481 ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc
      541 ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg
      601 tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc
      661 aaaacaaaag taagaccacc gcacagcaag cggccgctga tcttcagacc tggaggagga
      721 gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca
      781 ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg
      841 ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg
      901 tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac
      961 aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc
     1021 aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg
     1081 gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt
     1141 tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga
     1201 gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa
     1261 gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt
     1321 aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta
     1381 ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca
     1441 ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccttgcgcct tttccaaggc
     1501 agccctgggt ttgcgcaggg acgcggctgc tctgggcgtg gttccgggaa acgcagcggc
     1561 gccgaccctg ggtctcgcac attcttcacg tccgttcgca gcgtcacccg gatcttcgcc
     1621 gctacccttg tgggcccccc ggcgacgctt cctgctccgc ccctaagtcg ggaaggttcc
     1681 ttgcggttcg cggcgtgccg gacgtgacaa acggaagccg cacgtctcac tagtaccctc
     1741 gcagacggac agcgccaggg agcaatggca gcgcgccgac cgcgatgggc tgtggccaat
     1801 agcggctgct cagcagggcg cgccgagagc agcggccggg aaggggcggt gcgggaggcg
     1861 gggtgtgggg cggtagtgtg ggccctgttc ctgcccgcgc ggtgttccgc attctgcaag
     1921 cctccggagc gcacgtcggc agtcggctcc ctcgttgacc gaatcaccga cctctctccc
     1981 cagggggtac caccatggcc aagcctttgt ctcaagaaga atccaccctc attgaaagag
     2041 caacggctac aatcaacagc atccccatct ctgaagacta cagcgtcgcc agcgcagctc
     2101 tctctagcga cggccgcatc ttcactggtg tcaatgtata tcattttact gggggacctt
     2161 gtgcagaact cgtggtgctg ggcactgctg ctgctgcggc agctggcaac ctgacttgta
     2221 tcgtcgcgat cggaaatgag aacaggggca tcttgagccc ctgcggacgg tgccgacagg
     2281 tgcttctcga tctgcatcct gggatcaaag ccatagtgaa ggacagtgat ggacagccga
     2341 cggcagttgg gattcgtgaa ttgctgccct ctggttatgt gtgggagggc ctgcagctgc
     2401 agtagtaaga attctagatc ttgagacaaa tggcagtatt catccacaat tttaaaagaa
     2461 aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca
     2521 tacaaactaa agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca
     2581 gggacagcag agatccactt tggcgccggc tcgagggggc ccggggttat taatagtaat
     2641 caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg
     2701 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt
     2761 atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac
     2821 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg
     2881 acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact
     2941 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt
     3001 ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc
     3061 ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc
     3121 gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata
     3181 taagcagagc tctctggcta actgtcggga tcaacaagtt tgtacaaaaa agcaggctgg
     3241 cgccggaacc aattcagtcg actggatccg ccaccatggg cggagctcct gctccagacg
     3301 ctcacagcgc cccacctgga aacgattctg ccggaggcag tgagtaccat gccccagctg
     3361 gatatcaagt gaatccaccc taccaccccg tgcatgggta tgaggaacag tgcagctcca
     3421 tctacatcta ctatggggcc ctgtgggagc aggaaacagc taggggcttc cagtggtttg
     3481 ccgtgttcct gtctgccctg tttctggctt tctacggctg gcacgcctat aaggccagcg
     3541 tgggatggga ggaagtgtac gtgtgctccg tggagctgat caaagtgatt ctggagatct
     3601 atttcgagtt caccagtcct gctatgctgt tcctgtacgg agggaacatt accccatggc
     3661 tgagatatgc cgaatggctg ctgacatgtc ccgtgatcct gattcatctg tctaacatca
     3721 ccggcctgag tgaggcatac aataagcgga caatggctct gctggtgtcc gacctgggaa
     3781 ctatttgcat gggagtgaca gccgctctgg ccactgggtg ggtgaagtgg ctgttttact
     3841 gtatcggcct ggtgtatgga acccagacat tctacaacgc tggaatcatc tacgtggagt
     3901 cttactatat catgcctgcc ggcggctgta agaaactggt gctggccatg actgccgtgt
     3961 actattctag ttggctgatg tttcccggcc tgttcatctt tgggcctgaa ggcatgcaca
     4021 ccctgagcgt ggctgggtcc actattggcc ataccatcgc cgacctgctg tccaagaata
     4081 tttggggact gctggggcac ttcctgcgga tcaaaattca cgagcatatc attatgtacg
     4141 gcgatatcag gagaccagtg agctcccagt ttctgggacg caaggtggac gtgctggcct
     4201 tcgtgacaga ggaagataaa gtggcggccg ccaagagcag gatcaccagc gagggcgagt
     4261 acatccccct ggaccagatc gacatcaacg tggtgagcaa gggcgaggag ctgttcaccg
     4321 gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt
     4381 ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc atttgcacca
     4441 ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt
     4501 gcttcagccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg
     4561 aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg
     4621 ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact
     4681 tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg
     4741 tctatatcat ggccgacaag cagaagaacg gcatcaaggt gaacttcaag atccgccaca
     4801 acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg
     4861 acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccgcc ctgagcaaag
     4921 accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca
     4981 ctctcggcat ggacgagctg tacaagtaag aattcgcggc cgcactcgag atatctagac
     5041 ccagctttct tgtacaaagt ggttggtaag cctatcccta accctctcct cggtctcgat
     5101 tctacgtagt aatgagctag cgctaaccgg tggcgcgtta agtcgacaat caacctctgg
     5161 attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat
     5221 gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt
     5281 tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca
     5341 ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg
     5401 ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg
     5461 aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca
     5521 attccgtggt gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca
     5581 cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc
     5641 ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc
     5701 agacgagtcg gatctccctt tgggccgcct ccccgcgtcg actttaagac caatgactta
     5761 caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat
     5821 tcactcccaa cgaagacaag atctgctttt tgcttgtact gggtctctct ggttagacca
     5881 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
     5941 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
     6001 atccctcaga cccttttagt cagtgtggaa aatctctagc agtacgtata gtagttcatg
     6061 tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggaa
     6121 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
     6181 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
     6241 tcatgtctgg ctctagctat cccgccccta actccgccca tcccgcccct aactccgccc
     6301 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
     6361 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggg
     6421 acgtacccaa ttcgccctat agtgagtcgt attacgcgcg ctcactggcc gtcgttttac
     6481 aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc
     6541 ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc
     6601 gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg
     6661 tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt
     6721 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc
     6781 tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg
     6841 gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg
     6901 agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct
     6961 cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg
     7021 agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttagg
     7081 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
     7141 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
     7201 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg
     7261 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
     7321 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt
     7381 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt
     7441 attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa
     7501 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag
     7561 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac
     7621 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
     7681 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
     7741 cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac
     7801 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact
     7861 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
     7921 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
     7981 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
     8041 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta
     8101 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
     8161 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
     8221 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     8281 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     8341 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
     8401 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     8461 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     8521 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     8581 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     8641 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     8701 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     8761 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     8821 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     8881 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga
     8941 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga
     9001 agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg
     9061 cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt
     9121 gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt
     9181 gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc
     9241 caagcgcgca attaaccctc actaaaggga acaaaagctg gagctgcaag cttaatgtag
     9301 tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca acatgcctta
     9361 caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta cgatcgtgcc
     9421 ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga attgccgcat
     9481 tgcagagata ttgtatttaa gtgcctagct cgatacataa ac
//
