LOCUS       sequence-104124-         804 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses RNA-Guided, Nuclease-Inactive 
            VP64:dCas9-BFP:VP64—VdC9BV—Fusion Protein to Enable Transactivation 
            of Endogenous Genes.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 804)
  AUTHORS   Chakraborty S, Ji H, Kabadi AM, Gersbach CA, Christoforou N, Leong 
            KW
  TITLE     A CRISPR/Cas9-Based System for Reprogramming Cell Lineage 
            Specification.
  JOURNAL   Stem Cell Reports. 2014 Dec 9;3(6):940-7. doi: 
            10.1016/j.stemcr.2014.09.013. Epub 2014 Oct 23.
  PUBMED    25448066
REFERENCE   2  (bases 1 to 804)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stemcr.2014.09.013"; journalName: "Stem Cell Reports"; 
            date: "2014-12-9- 9"; volume: "3"; issue: "6"; pages: "940-7"
COMMENT     Sequence Label: TetO-FUW-VdC9BV
FEATURES             Location/Qualifiers
     source          1..804
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(21..41)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 atcagctcgc tggaactacc taccttgcgc tttttcttgg gagctccctc atcccctccg
       61 agctgtgaga ggtcaatccg agtttcgtaa agtccggtga tgctctggtg gatgagggtg
      121 gcgtccagca cttctttggt ggaggtatag cgtttccggt cgatggtggt gtcgaagtac
      181 ttgaatgcgg caggggcacc aagattggtg agggtgaaca ggtggatgat attctcagcc
      241 tgctcccgaa ttggtttgtc tctgtgctta ttgtatgcgc taaggacttt atcgaggttg
      301 gcatcagcga gaatgacgcg tttagaaaac tcggaaatct gctcaatgat ctcgtccaga
      361 taatgcttat gctgctccac aaacagttgc ttttgctcgt tgtcctcggg gctccccttc
      421 agcttctcgt aatggcttgc aaggtagagg aaattcacgt acttggaggg gagagccagc
      481 tcattaccct tctgaagttc gccagcggag gcgagcatcc gcttccgccc attctccagt
      541 tcgaacaggc tatactttgg gagcttgatg ataaggtcct tcttgacttc cttgtaacct
      601 ttggcttcga gaaagtcaat tgggttcttc tcgaaggagc tgcgctccat aatggtgata
      661 cccagcagtt ccttcacgct cttgagcttc ttagactttc ctttttccac cttagccacg
      721 accaggacag aataagccac agttggtgaa tcgaatccgc cgtacttctt agggtcccaa
      781 tccttcttcc ttgcaatgag cttg
//
