LOCUS       sequence-104125-         829 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  PhoRadAN13 intein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 829)
  AUTHORS   Aranko AS, Oeemig JS, Zhou D, Kajander T, Wlodawer A, Iwai H
  TITLE     Structure-based engineering and comparison of novel split inteins 
            for protein ligation.
  JOURNAL   Mol Biosyst. 2014 May;10(5):1023-34. doi: 10.1039/c4mb00021h.
  PUBMED    24574026
REFERENCE   2  (bases 1 to 829)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Mol Biosyst"; date: "2014-05"; volume: "10"; issue: "5"; pages: 
            "1023-34"
COMMENT     Sequence Label: pSARSF53-455
FEATURES             Location/Qualifiers
     source          1..829
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             43..60
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             67..357
                     /codon_start=1
                     /gene="S. cerevisiae SMT3 (truncated)"
                     /product="cleavable ubiquitin-like protein tag"
                     /label=SUMO
                     /translation="SDSEVNQEAKPEVKPEVKPETHINLKVSDGSSEIFFKIKKTTPLR
                     RLMEAFAKRQGKEMDSLRFLYDGIRIQADQTPEDLDMEDNDIIETHREQIGG"
     CDS             434..478
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     primer_bind     complement(516..534)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      530..577
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 ttttgtttaa ctttaataag gagatatacc atgggcagca gccatcatca tcatcatcac
       61 agcactagtg actcagaagt caatcaagaa gctaagccag aggtcaagcc agaagtcaag
      121 cctgagactc acatcaattt aaaggtgtcc gatggatctt cagaaatctt ctttaagatc
      181 aaaaagacca ctcctttaag aaggctgatg gaagcgttcg ctaaaagaca gggtaaggaa
      241 atggactcct taagattctt gtacgacggt attagaattc aagctgatca gacccctgaa
      301 gatttggaca tggaggataa cgatattatt gagactcaca gagaacagat tggtggatcc
      361 gggaagtgct ttgctaggga taccgaagtt tattatgaaa acgattaagc ttgcggccgc
      421 actcgagtct ggtaaagaaa ccgctgctgc gaaatttgaa cgccagcaca tggactcgtc
      481 tactagcgca gcttaattaa cctaggctgc tgccaccgct gagcaataac tagcataacc
      541 ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaacctcagg catttgagaa
      601 gcacacggtc acactgcttc cggtagtcaa taaaccggta aaccagcaat agacataagc
      661 ggctatttaa cgaccctgcc ctgaaccgac gacaagctga cgaccgggtc tccgcaagtg
      721 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
      781 atatgtatcc gctcatgaat taattcttag aaaaactcat cgagcatca
//
