LOCUS       sequence-104131-         869 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  A human codon-optimized SpCas9 and chimeric guide RNA expression 
            plasmid..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 869)
  AUTHORS   Cong L, Ran FA, Cox D, Lin S, Barretto R, Habib N, Hsu PD, Wu X, 
            Jiang W, Marraffini LA, Zhang F
  TITLE     Multiplex Genome Engineering Using CRISPR/Cas Systems.
  JOURNAL   Science. 2013 Jan 3.
  PUBMED    23287718
REFERENCE   2  (bases 1 to 869)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2013 Jan 3."
COMMENT     Sequence Label: pX330-U6-Chimeric_BB-CBh-hSpCas9
FEATURES             Location/Qualifiers
     source          1..869
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 aagaaactgg tggacagcac cgacaaggcc gacctgcggc tgatctatct ggccctggcc
       61 cacatgatca agttccgggg ccacttcctg atcgagggcg acctgaaccc cgacaacagc
      121 gacgtggaca agctgttcat ccagctggtg cagacctaca accagctgtt cgaggaaaac
      181 cccatcaacg ccagcggcgt ggacgccaag gccatcctgt ctgccagact gagcaagagc
      241 agacggctgg aaaatctgat cgcccagctg cccggcgaga agaagaatgg cctgttcgga
      301 aacctgattg ccctgagcct gggcctgacc cccaacttca agagcaactt cgacctggcc
      361 gaggatgcca aactgcagct gagcaaggac acctacgacg acgacctgga caacctgctg
      421 gcccagatcg gcgaccagta cgccgacctg tttctggccg ccaagaacct gtccgacgcc
      481 atcctgctga gcgacatcct gagagtgaac accgagatca ccaaggcccc cctgagcgcc
      541 tctatgatca agagatacga cgagcaccac caggacctga ccctgctgaa agctctcgtg
      601 cggcagcagc tgcctgagaa gtacaaagag attttcttcg accagagcaa gaacggctac
      661 gccggctaca ttgacggcgg agccagccag gaagagttct acaagttcat caagcccatc
      721 ctggaaaaga tggacggcac cgaggaactg ctcgtgaagc tgaacagaga ggacctgctg
      781 cggaagcagc ggaccttcga caacggcagc atcccccacc agatccacct gggagagctg
      841 cacgccattc tgcggcggca ggaagattt
//
