LOCUS       sequence-104144-         864 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Recombinase-mediated cassette exchange in ES cells to insert the 
            Flpo recombinase gene at the mouse Pvalb gene stop codon.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 864)
  AUTHORS   Madisen L, Garner AR, Shimaoka D, Chuong AS, Klapoetke NC, Li L, van
            der Bourg A, Niino Y, Egolf L, Monetti C, Gu H, Mills M, Cheng A, 
            Tasic B, Nguyen TN, Sunkin SM, Benucci A, Nagy A, Miyawaki A, 
            Helmchen F, Empson RM, Knopfel T, Boyden ES, Reid RC, Carandini M, 
            Zeng H
  TITLE     Transgenic mice for intersectional targeting of neural sensors and 
            effectors with high specificity and performance.
  JOURNAL   Neuron. 2015 Mar 4;85(5):942-58. doi: 10.1016/j.neuron.2015.02.022.
  PUBMED    25741722
REFERENCE   2  (bases 1 to 864)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2015.02"; journalName: "Neuron"; date: "2015-03-4-
            4"; volume: "85"; issue: "5"; pages: "942-58"
COMMENT     Sequence Label: Pvalb-2A-Flpo Flp-in replacement vector
FEATURES             Location/Qualifiers
     source          1..864
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             320..373
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             380..400
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 aatagtatag gaacttcttt cggccggcct cagggggttc tcagagacaa tgaatgacga
       61 gttctaagtc taccctgacc cctccggtca aacagcagct gttcaaagaa acggccaggc
      121 tcagagcctc cattccctca tccacagagc aggcatggtg actaacaaca cagtcgcagc
      181 tgaagcccaa ggagatattg tactcggagg gtttaagata gctggtgcac ggcagacgtg
      241 gggatgcttg ccgaagataa gagtgtgtca tttttctttt gcagaattct ccactctggt
      301 ggctgaaagc ggttccggag agggcagagg aagtcttcta acatgcggtg acgtggagga
      361 gaatcccggc cctatggctc ctaagaagaa gaggaaggtg atgagccagt tcgacatcct
      421 gtgcaagacc ccccccaagg tgctggtgcg gcagttcgtg gagagattcg agaggcccag
      481 cggcgagaag atcgccagct gtgccgccga gctgacctac ctgtgctgga tgatcaccca
      541 caacggcacc gccatcaaga gggccacctt catgagctac aacaccatca tcagcaacag
      601 cctgagcttc gacatcgtga acaagagcct gcagttcaag tacaagaccc agaaggccac
      661 catcctggag gccagcctga agaagctgat ccccgcctgg gagttcacca tcatccctta
      721 caacggccag aagcaccaga gcgacatcac cgacatcgtg tccagcctgc agctgcagtt
      781 cgagagcagc gaggaggccg acaagggcaa cagccacagc aagaagatgc tgaaggccct
      841 gctgtccgag ggcgagagca tctg
//
