LOCUS       sequence-104146-         887 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  PhoRadAN13 intein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 887)
  AUTHORS   Aranko AS, Oeemig JS, Zhou D, Kajander T, Wlodawer A, Iwai H
  TITLE     Structure-based engineering and comparison of novel split inteins 
            for protein ligation.
  JOURNAL   Mol Biosyst. 2014 May;10(5):1023-34. doi: 10.1039/c4mb00021h.
  PUBMED    24574026
REFERENCE   2  (bases 1 to 887)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Mol Biosyst"; date: "2014-05"; volume: "10"; issue: "5"; pages: 
            "1023-34"
COMMENT     Sequence Label: pSARSF53-455
FEATURES             Location/Qualifiers
     source          1..887
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(16..60)
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             complement(137..427)
                     /codon_start=1
                     /gene="S. cerevisiae SMT3 (truncated)"
                     /product="cleavable ubiquitin-like protein tag"
                     /label=SUMO
                     /translation="SDSEVNQEAKPEVKPEVKPETHINLKVSDGSSEIFFKIKKTTPLR
                     RLMEAFAKRQGKEMDSLRFLYDGIRIQADQTPEDLDMEDNDIIETHREQIGG"
     CDS             complement(434..451)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     protein_bind    507..531
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(531..550)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(532..550)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        596..673
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     complement(693..712)
                     /label=LacI-R
                     /note="LacI, reverse primer"
ORIGIN
        1 agctgcgcta gtagacgagt ccatgtgctg gcgttcaaat ttcgcagcag cggtttcttt
       61 accagactcg agtgcggccg caagcttaat cgttttcata ataaacttcg gtatccctag
      121 caaagcactt cccggatcca ccaatctgtt ctctgtgagt ctcaataata tcgttatcct
      181 ccatgtccaa atcttcaggg gtctgatcag cttgaattct aataccgtcg tacaagaatc
      241 ttaaggagtc catttcctta ccctgtcttt tagcgaacgc ttccatcagc cttcttaaag
      301 gagtggtctt tttgatctta aagaagattt ctgaagatcc atcggacacc tttaaattga
      361 tgtgagtctc aggcttgact tctggcttga cctctggctt agcttcttga ttgacttctg
      421 agtcactagt gctgtgatga tgatgatgat ggctgctgcc catggtatat ctccttatta
      481 aagttaaaca aaattatttc tacaggggaa ttgttatccg ctcacaattc ccctatagtg
      541 agtcgtatta atttcctaat gcaggagtcg cataagggag agcgtcgaga tcccggacac
      601 catcgaatgg cgcaaaacct ttcgcggtat ggcatgatag cgcccggaag agagtcaatt
      661 cagggtggtg aatgtgaaac cagtaacgtt atacgatgtc gcagagtatg ccggtgtctc
      721 ttatcagacc gtttcccgcg tggtgaacca ggccagccac gtttctgcga aaacgcggga
      781 aaaagtggaa gcggcgatgg cggagctgaa ttacattccc aaccgcgtgg cacaacaact
      841 ggcgggcaaa cagtcgttgc tgattggcgt tgccacctcc agtctgg
//
