LOCUS       sequence-104147-         941 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmid for bacterial overexpression of RBPMS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 941)
  AUTHORS   Farazi TA, Leonhardt CS, Mukherjee N, Mihailovic A, Li S, Max KE, 
            Meyer C, Yamaji M, Cekan P, Jacobs NC, Gerstberger S, Bognanni C, 
            Larsson E, Ohler U, Tuschl T
  TITLE     Identification of the RNA recognition element of the RBPMS family of
            RNA-binding proteins and their transcriptome-wide mRNA targets.
  JOURNAL   RNA. 2014 Jul;20(7):1090-102. doi: 10.1261/rna.045005.114. Epub 2014
            May 23.
  PUBMED    24860013
REFERENCE   2  (bases 1 to 941)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1261/rna.045005.114"; journalName: "RNA"; date: "2014-07"; 
            volume: "20"; issue: "7"; pages: "1090-102"
COMMENT     Sequence Label: pet23a_RBPMS_FL
FEATURES             Location/Qualifiers
     source          1..941
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     208..227
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        208..226
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             258..280
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             288..320
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
ORIGIN
        1 ccggaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg
       61 cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag
      121 cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg
      181 ctgcccgaga tctcgatccc gcgaaattaa tacgactcac tatagggaga ccacaacggt
      241 ttccctctag aaataatttt gtttaacttt aagaaggaga tatacatatg gctagcatga
      301 ctggtggaca gcaaatgggt cgcggatccg aattcgagct ccgtcgaccc atgaacaacg
      361 gcggcaaagc cgagaaggag aacaccccga gcgaggccaa ccttcaggag gaggaggtcc
      421 ggaccctatt tgtcagtggc cttcctctgg atatcaaacc tcgggagctc tatctgcttt
      481 tcagaccatt taagggctat gagggttctc ttataaagct cacatctaaa cagcctgtag
      541 gttttgtcag ttttgacagt cgctcagaag cagaggctgc aaagaatgct ttgaatggca
      601 tccgcttcga tcctgaaatt ccgcaaacac tacgactaga gtttgctaag gcaaacacga
      661 agatggccaa gaacaaactc gtagggactc caaaccccag tactcctctg cccaacactg
      721 tacctcagtt cattgccaga gagccatatg agctcacagt gcctgcactt taccccagta
      781 gccctgaagt gtgggccccg taccctctgt acccagcgga gttagcgcct gctctacctc
      841 ctcctgcttt cacctatccc gcttcactgc atgcccagat gcgctggctc cctccctccg
      901 aggctacttc tcagggctgg aagtcccgtc agttctgcgc g
//
