LOCUS       sequence-104148-         838 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmid for bacterial overexpression of RBPMS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 838)
  AUTHORS   Farazi TA, Leonhardt CS, Mukherjee N, Mihailovic A, Li S, Max KE, 
            Meyer C, Yamaji M, Cekan P, Jacobs NC, Gerstberger S, Bognanni C, 
            Larsson E, Ohler U, Tuschl T
  TITLE     Identification of the RNA recognition element of the RBPMS family of
            RNA-binding proteins and their transcriptome-wide mRNA targets.
  JOURNAL   RNA. 2014 Jul;20(7):1090-102. doi: 10.1261/rna.045005.114. Epub 2014
            May 23.
  PUBMED    24860013
REFERENCE   2  (bases 1 to 838)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1261/rna.045005.114"; journalName: "RNA"; date: "2014-07"; 
            volume: "20"; issue: "7"; pages: "1090-102"
COMMENT     Sequence Label: pet23a_RBPMS_FL
FEATURES             Location/Qualifiers
     source          1..838
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(11..58)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     54..72
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     CDS             complement(125..142)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(776..808)
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     RBS             816..838
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 tccctttcag caaaaaaccc ctcaagaccc gtttagaggc cccaaggggt tatgctagtt
       61 attgctcagc ggtggcagca gccaactcag cttcctttcg ggctttgtta gcagccggat
      121 ctcagtggtg gtggtggtgg tgctcgagtg cggccgcgca gaactgacgg gacttccagc
      181 cctgagaagt agcctcggag ggagggagcc agcgcatctg ggcatgcagt gaagcgggat
      241 aggtgaaagc aggaggaggt agagcaggcg ctaactccgc tgggtacaga gggtacgggg
      301 cccacacttc agggctactg gggtaaagtg caggcactgt gagctcatat ggctctctgg
      361 caatgaactg aggtacagtg ttgggcagag gagtactggg gtttggagtc cctacgagtt
      421 tgttcttggc catcttcgtg tttgccttag caaactctag tcgtagtgtt tgcggaattt
      481 caggatcgaa gcggatgcca ttcaaagcat tctttgcagc ctctgcttct gagcgactgt
      541 caaaactgac aaaacctaca ggctgtttag atgtgagctt tataagagaa ccctcatagc
      601 ccttaaatgg tctgaaaagc agatagagct cccgaggttt gatatccaga ggaaggccac
      661 tgacaaatag ggtccggacc tcctcctcct gaaggttggc ctcgctcggg gtgttctcct
      721 tctcggcttt gccgccgttg ttcatgggtc gacggagctc gaattcggat ccgcgaccca
      781 tttgctgtcc accagtcatg ctagccatat gtatatctcc ttcttaaagt taaacaaa
//
