LOCUS       sequence-104150-         849 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Destination vector with RBPMS for stable cell line generation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 849)
  AUTHORS   Farazi TA, Leonhardt CS, Mukherjee N, Mihailovic A, Li S, Max KE, 
            Meyer C, Yamaji M, Cekan P, Jacobs NC, Gerstberger S, Bognanni C, 
            Larsson E, Ohler U, Tuschl T
  TITLE     Identification of the RNA recognition element of the RBPMS family of
            RNA-binding proteins and their transcriptome-wide mRNA targets.
  JOURNAL   RNA. 2014 Jul;20(7):1090-102. doi: 10.1261/rna.045005.114. Epub 2014
            May 23.
  PUBMED    24860013
REFERENCE   2  (bases 1 to 849)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1261/rna.045005.114"; journalName: "RNA"; date: "2014-07"; 
            volume: "20"; issue: "7"; pages: "1090-102"
COMMENT     Sequence Label: pFRT-TODestFLAGHA_RBPMS
FEATURES             Location/Qualifiers
     source          1..849
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    18..36
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     46..70
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             170..193
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             194..220
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    236..260
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 tatcagtgat agagatctcc ctatcagtga tagagatcgt cgacgagctc gtttagtgaa
       61 ccgtcagatc gcctggagac gccatccacg ctgttttgac ctccatagaa gacaccggga
      121 ccgatccagc ctccggactc tagcgtttaa acttaagctt ggtaccatgg actacaagga
      181 cgacgatgac aagtaccctt atgacgtgcc cgattacgct ttgaaccaga tatcaacaag
      241 tttgtacaaa aaagcaggct ccaccatggg aaccaattca gtcgacatga acaacggcgg
      301 caaagccgag aaggagaaca ccccgagcga ggccaacctt caggaggagg aggtccggac
      361 cctatttgtc agtggccttc ctctggatat caaacctcgg gagctctatc tgcttttcag
      421 accatttaag ggctatgagg gttctcttat aaagctcaca tctaaacagc ctgtaggttt
      481 tgtcagtttt gacagtcgct cagaagcaga ggctgcaaag aatgctttga atggcatccg
      541 cttcgatcct gaaattccgc aaacactacg actagagttt gctaaggcaa acacgaagat
      601 ggccaagaac aaactcgtag ggactccaaa ccccagtact cctctgccca acactgtacc
      661 tcagttcatt gccagagagc catatgagct cacagtgcct gcactttacc ccagtagccc
      721 tgaagtgtgg gccccgtacc ctctgtaccc agcggagtta gcgcctgctc tacctcctcc
      781 tgctttcacc tatcccgctt cactgcatgc ccagatgcgc tggctccctc cctccgaggc
      841 tacttctca
//
