LOCUS       sequence-104160-         785 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Recombinase-mediated cassette exchange in ES cells to insert the 
            Flpo recombinase gene at the mouse Pvalb gene stop codon.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 785)
  AUTHORS   Madisen L, Garner AR, Shimaoka D, Chuong AS, Klapoetke NC, Li L, van
            der Bourg A, Niino Y, Egolf L, Monetti C, Gu H, Mills M, Cheng A, 
            Tasic B, Nguyen TN, Sunkin SM, Benucci A, Nagy A, Miyawaki A, 
            Helmchen F, Empson RM, Knopfel T, Boyden ES, Reid RC, Carandini M, 
            Zeng H
  TITLE     Transgenic mice for intersectional targeting of neural sensors and 
            effectors with high specificity and performance.
  JOURNAL   Neuron. 2015 Mar 4;85(5):942-58. doi: 10.1016/j.neuron.2015.02.022.
  PUBMED    25741722
REFERENCE   2  (bases 1 to 785)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2015.02"; journalName: "Neuron"; date: "2015-03-4-
            4"; volume: "85"; issue: "5"; pages: "942-58"
COMMENT     Sequence Label: Pvalb-2A-Flpo Flp-in replacement vector
FEATURES             Location/Qualifiers
     source          1..785
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(683..702)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
ORIGIN
        1 agcttgcatg caggcctctg cagtcgacga gctcgaagtt cctatacctt ttgaagaata
       61 ggaacttcga ttcaggccta ggggctagca cttacactga cggtgtcgtc catcacagtt
      121 tgccagtgat acacatgggg atcagcaatc gcgcatatga aatcacgcca tgtagtgtat
      181 tgaccgattc cttgcggtcc gaatgggccg aacccgctcg tctggctaag atcggccgca
      241 gcgatcgcat ccatggcctc cgcgaccggc tgcagaacag cgggcagttc ggtttcaggc
      301 aggtcttgca acgtgacacc ctgtgcacgg cgggagatgc aataggtcag gctctcgctg
      361 aattccccaa tgtcaagcac ttccggaatc gggagcgcgg ccgatgcaaa gtgccgataa
      421 acataacgat ctttgtagaa accatcggcg cagctattta cccgcaggac atatccacgc
      481 cctcctacat cgaagctgaa agcacgagat tcttcgccct ccgagagctg catcaggtcg
      541 gagacgctgt cgaacttttc gatcagaaac ttctcgacag acgtcgcggt gagttcaggc
      601 tttttcatgg tggcggctag cggcgattgc aggtcgaaag gcccggagat gaggaagagg
      661 agaacagcgc ggcagacgtg cgcttttgaa gcgtgcagaa tgccgggcct ccggaggacc
      721 ttcgggcgcc cgccccgccc ctgagcccgc ccctgagccc gcccccggac ccaccccttc
      781 ccagc
//
