LOCUS       sequence-104176-         775 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Recombinase-mediated cassette exchange in ES cells to insert the 
            Flpo recombinase gene at the mouse Pvalb gene stop codon.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 775)
  AUTHORS   Madisen L, Garner AR, Shimaoka D, Chuong AS, Klapoetke NC, Li L, van
            der Bourg A, Niino Y, Egolf L, Monetti C, Gu H, Mills M, Cheng A, 
            Tasic B, Nguyen TN, Sunkin SM, Benucci A, Nagy A, Miyawaki A, 
            Helmchen F, Empson RM, Knopfel T, Boyden ES, Reid RC, Carandini M, 
            Zeng H
  TITLE     Transgenic mice for intersectional targeting of neural sensors and 
            effectors with high specificity and performance.
  JOURNAL   Neuron. 2015 Mar 4;85(5):942-58. doi: 10.1016/j.neuron.2015.02.022.
  PUBMED    25741722
REFERENCE   2  (bases 1 to 775)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2015.02"; journalName: "Neuron"; date: "2015-03-4-
            4"; volume: "85"; issue: "5"; pages: "942-58"
COMMENT     Sequence Label: Pvalb-2A-Flpo Flp-in replacement vector
FEATURES             Location/Qualifiers
     source          1..775
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    70..103
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
ORIGIN
        1 gcgcatgctc cagactgcct tgggaaaagc gcctccccta cccggtagaa ttcgatgcgg
       61 ccgcccaaac gcgcccgggg agcccaaggg cacgccctgg cacctaaaac atatggcttc
      121 tgcaataaga tctatgccaa gcttttacgg cgcgccttcc ttcctgaatg tggatacagt
      181 gtgacacaat gtagcctgcc tcctggggct tccctaccaa tgataggccg caccctctta
      241 gagagccaaa agaaaccgct ctttccttaa gtttctcttt tcaggtattt tatcacagca
      301 aagtcaaaag caattgatag agtgtcattg gagtcctttg atctagctag tcctgaagga
      361 ctcaacccct tcccttccct tctgctcttt aaaaggcagt ttctgtatct ttaagatgga
      421 cgatccatca cccccatctc cttgtgggaa aggtgcagag attgaacgag gtggcccaca
      481 tgagcactca gtaccaagca ggcaggagat atcggggcgt tgtcctttga cttatctcac
      541 tttttctact atacccccac tgccctaaaa agaaagaaaa aaaacttgcc aaaccaacac
      601 cctgccaggc ctgggtcctc cctacaggtg gtgtccgatt ggtacagcct ttattgtttc
      661 tccagcattt ccagaagaat ggcgtcatcc gagggccata gaggatgggg gagtaaaaaa
      721 taacacaaac aaactgaaca gaaactcagg agggccgcga gaaggactga gatgg
//
