LOCUS       sequence-104180-         779 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Target a Cre-dependent GFP expression cassette to the stop codon of 
            the mouse Snap25 gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 779)
  AUTHORS   Madisen L, Garner AR, Shimaoka D, Chuong AS, Klapoetke NC, Li L, van
            der Bourg A, Niino Y, Egolf L, Monetti C, Gu H, Mills M, Cheng A, 
            Tasic B, Nguyen TN, Sunkin SM, Benucci A, Nagy A, Miyawaki A, 
            Helmchen F, Empson RM, Knopfel T, Boyden ES, Reid RC, Carandini M, 
            Zeng H
  TITLE     Transgenic mice for intersectional targeting of neural sensors and 
            effectors with high specificity and performance.
  JOURNAL   Neuron. 2015 Mar 4;85(5):942-58. doi: 10.1016/j.neuron.2015.02.022.
  PUBMED    25741722
REFERENCE   2  (bases 1 to 779)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2015.02"; journalName: "Neuron"; date: "2015-03-4-
            4"; volume: "85"; issue: "5"; pages: "942-58"
COMMENT     Sequence Label: Snap25-LSL-2A-GFP targeting vector
FEATURES             Location/Qualifiers
     source          1..779
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(2..67)
                     /codon_start=1
                     /product="2A peptide from foot-and-mouth disease virus 
                     polyprotein"
                     /label=F2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="VKQTLNFDLLKLAGDVESNPGP"
     protein_bind    complement(97..130)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     polyA_signal    137..598
                     /label=PGK poly(A) signal
                     /note="mouse phosphoglycerate kinase 1 polyadenylation 
                     signal"
     protein_bind    complement(624..657)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 cgggccctgg gttggactcc acgtctcccg ccaacttgag aaggtcaaaa ttcaaagtct
       61 gtttcaccgg tgctccagaa cctctcttag cccgtgataa cttcgtatag catacattat
      121 acgaagttat gaagctttct gatggaatta gaacttggca aaacaatact gagaatgaag
      181 tgtatgtgga acagaggctg ctgatctcgt tcttcaggct atgaaactga cacatttgga
      241 aaccacagta cttagaacca caaagtggga atcaagagaa aaacaatgat cccacgagag
      301 atctatagat ctatagatca tgagtgggag gaatgagctg gcccttaatt tggttttgct
      361 tgtttaaatt atgatatcca actatgaaac attatcataa agcaatagta aagagccttc
      421 agtaaagagc aggcatttat ctaatcccac cccaccccca cccccgtagc tccaatcctt
      481 ccattcaaaa tgtaggtact ctgttctcac ccttcttaac aaagtatgac aggaaaaact
      541 tccattttag tggacatctt tattgtttaa tagatcatca atttctgcag acttacagcg
      601 gatcccccta gttaatcagc ctgataactt cgtatagcat acattatacg aagttatacc
      661 acttcccagc atctttgttg cacgttggtt ggcttcatca attctggttt tgttggaatc
      721 agcctagaaa agaggagaga agagaggtct tagacgactc cttaaggtcc tattcatcc
//
