LOCUS       sequence-104193-        1027 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  docking site transgenesis plasmid for GoldenGate Cloning and insect 
            transgenesis, GFP marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1027)
  AUTHORS   Volohonsky G, Terenzi O, Soichot J, Naujoks DA, Nolan T, Windbichler
            N, Kapps D, Smidler AL, Vittu A, Costa G, Steinert S, Levashina EA, 
            Blandin SA, Marois E
  TITLE     Tools for Anopheles gambiae Transgenesis.
  JOURNAL   G3 (Bethesda). 2015 Apr 13. pii: g3.115.016808. doi: 
            10.1534/g3.115.016808.
  PUBMED    25869647
REFERENCE   2  (bases 1 to 1027)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2015 Apr 13. pii: g3.115.016808. doi: 
            10.1534/g3.115.016808."
COMMENT     Sequence Label: pDSAG
FEATURES             Location/Qualifiers
     source          1..1027
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    213..295
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(250..269)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     305..324
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    353..422
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     regulatory      937..946
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(988..1009)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta
       61 aaaaatgagc tgatttaaca aaaatttaac gcgggtctcc gcttctagac ataatcagcc
      121 ataccacatt tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc
      181 tgaaacataa aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt
      241 acaaataaag caatagcatc acaaatttca caaataaagc atttttcttc actgcattct
      301 agttgtggtt tgtccaaact catcaatgta tctcgacgat gtaggtcaca gtctcgaagc
      361 cgcggtgcgg gtgccagggc gtgcccttgg gctccccggg cgcgtactcc acctcaccca
      421 tctggtccat catgatgaac gggtcgaggt ggcggtagtt gatcccggcg aacgcgcggc
      481 gcaccgggaa gccctcgccc tcgaaaccgc tgggcgcggt ggtcacggtg agcacgggac
      541 gtgcgacggc gtcggcgggt gcggatacgc ggggcagcgt cagcgggttc tcgacggtca
      601 cggcgggcaa ttcctgcaga cttccggtat ctcgcgtttg tttgatcgca cggttcccac
      661 aatggttaat tcgagctcgc ccggggatct aattcaatta gagactaatt caattagagc
      721 taattcaatt aggatccaag cttatcgatt tcgaaccctc gaccgccgga gtataaatag
      781 aggcgcttcg tctacggagc gacaattcaa ttcaaacaag caaagtgaac acgtcgctaa
      841 gcgaaagcta agcaaataaa caagcgcagc tgaacaagct aaacaatcgg ggtaccgcta
      901 gagtcgacgg taccgcgggc ccgggatcca ccggtcgcca ccatggtgag caagggcgag
      961 gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac
     1021 aagttca
//
