LOCUS       sequence-104196-        1055 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  docking site transgenesis plasmid for GoldenGate Cloning and insect 
            transgenesis, GFP marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1055)
  AUTHORS   Volohonsky G, Terenzi O, Soichot J, Naujoks DA, Nolan T, Windbichler
            N, Kapps D, Smidler AL, Vittu A, Costa G, Steinert S, Levashina EA, 
            Blandin SA, Marois E
  TITLE     Tools for Anopheles gambiae Transgenesis.
  JOURNAL   G3 (Bethesda). 2015 Apr 13. pii: g3.115.016808. doi: 
            10.1534/g3.115.016808.
  PUBMED    25869647
REFERENCE   2  (bases 1 to 1055)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2015 Apr 13. pii: g3.115.016808. doi: 
            10.1534/g3.115.016808."
COMMENT     Sequence Label: pDSAG
FEATURES             Location/Qualifiers
     source          1..1055
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     5..21
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5..21
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     26..45
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        26..44
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    75..174
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     polyA_signal    235..356
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(246..265)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     300..319
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(523..544)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     872..891
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 ctgccaggaa acagctatga ccatgtaata cgactcacta taggggatat cgcggccgcc
       61 ctgcagctgg atggcaaata atgattttat tttgactgat agtgacctgt tcgttgcaac
      121 aaattgataa gcaatgcttt cttataatgc caactttgta caagaaagct gggtactcga
      181 gctccaccgc ggtggcggcc gctctaggcg cgccgtacgc gtatcgataa gctttaagat
      241 acattgatga gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg
      301 aaatttgtga tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttaaca
      361 acaacaattg cattcatttt atgtttcagg ttcaggggga ggtgtgggag gttttttaaa
      421 gcaagtaaaa cctctacaaa tgtggtatgg ctgatttgat ctagagtcgc ggccgcttta
      481 cttgtacagc tcgtccatgc cgagagtgat cccggcggcg gtcacgaact ccagcaggac
      541 catgtgatcg cgcttctcgt tggggtcttt gctcagggcg gactgggtgc tcaggtagtg
      601 gttgtcgggc agcagcacgg ggccgtcgcc gatgggggtg ttctgctggt agtggtcggc
      661 gagctgcacg ctgccgtcct cgatgttgtg gcggatcttg aagttcacct tgatgccgtt
      721 cttctgcttg tcggccatga tatagacgtt gtggctgttg tagttgtact ccagcttgtg
      781 ccccaggatg ttgccgtcct ccttgaagtc gatgcccttc agctcgatgc ggttcaccag
      841 ggtgtcgccc tcgaacttca cctcggcgcg ggtcttgtag ttgccgtcgt ccttgaagaa
      901 gatggtgcgc tcctggacgt agccttcggg catggcggac ttgaagaagt cgtgctgctt
      961 catgtggtcg gggtagcggc tgaagcactg cacgccgtag gtcagggtgg tcacgagggt
     1021 gggccagggc acgggcagct tgccggtggt gcaga
//
