LOCUS       sequence-104203-         972 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 972)
  AUTHORS   Zhang Y, Gao J, Chung KK, Huang H, Dawson VL, Dawson TM
  TITLE     Parkin functions as an E2-dependent ubiquitin- protein ligase and 
            promotes the degradation of the synaptic vesicle-associated protein,
            CDCrel-1.
  JOURNAL   Proc Natl Acad Sci U S A 2000 Nov 21;97(24):13354-9.
  PUBMED    11078524
REFERENCE   2  (bases 1 to 972)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A"; date: "2000-11-21"; volume: "97(24)"; pages: 
            "13354-9"
COMMENT     Sequence Label: pRK5-Myc-Parkin
FEATURES             Location/Qualifiers
     source          1..972
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        225..243
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     225..242
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     256..275
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      360..369
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             369..398
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac
       61 cgggaccgat ccagcctccg cggccgggaa cggtgcattg gaacgcggat tccccgtgcc
      121 aagagtgacg taagtaccgc ctatagagtc tataggccca cccccttggc ttcgttagaa
      181 cgcggctaca attaatacat aaccttatgt atcatacaca tacgatttag gtgacactat
      241 agaataacat ccactttgcc tttctctcca caggtgtcca ctcccaggtc caactgcacc
      301 tcggttctat cgattgaatt cccgaaccga cagtcggtct cttcaccaag gccattcgcg
      361 ccaccatgga gcaaaagctc atttctgagg aagatctcaa tggtggtggt ggtgggtcga
      421 ccatgatagt gtttgtcagg ttcaactcca gccatggttt cccagtggag gtcgattctg
      481 acaccagcat cttccagctc aaggaggtgg ttgctaagcg acagggggtt ccggctgacc
      541 agttgcgtgt gattttcgca gggaaggagc tgaggaatga ctggactgtg cagaattgtg
      601 acctggatca gcagagcatt gttcacattg tgcagagacc gtggagaaaa ggtcaagaaa
      661 tgaatgcaac tggaggcgac gaccccagaa acgcggcggg aggctgtgag cgggagcccc
      721 agagcttgac tcgggtggac ctcagcagct cagtcctccc aggagactct gtggggctgg
      781 ctgtcattct gcacactgac agcaggaagg actcaccacc agctggaagt ccagcaggta
      841 gatcaatcta caacagcttt tatgtgtatt gcaaaggccc ctgtcaaaga gtgcagccgg
      901 gaaaactcag ggtacagtgc agcacctgca ggcaggcaac gctcaccttg acccagggtc
      961 catcttgctg gg
//
