LOCUS       sequence-104208-         906 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 906)
  AUTHORS   Deardorff MA, Tan C, Conrad LJ, Klein PS
  TITLE     Frizzled-8 is expressed in the Spemann organizer and plays a role in
            early morphogenesis.
  JOURNAL   Development. 1998 Jul . 125(14):2687-700.
  PUBMED    9636083
REFERENCE   2  (bases 1 to 906)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Development. 1998 Jul . 125(14):2687-700."
COMMENT     Sequence Label: xfz8N/pCS2+
FEATURES             Location/Qualifiers
     source          1..906
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    126..147
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(162..167,168..185,186..192)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    200..216
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     205..227
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     224..240
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     224..240
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     259..279
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        261..279
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     polyA_signal    392..526
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(429..448)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     483..502
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg
       61 attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac
      121 gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt tatgctcccg
      181 gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac
      241 catgattacg ccaagcgcgc aattaaccct cactaaaggg aacaaaagct gggtaccggg
      301 cccaatgcat tggcgccgcg gccgcgaatt aaaaaacctc ccacacctcc ccctgaacct
      361 gaaacataaa atgaatgcaa ttgttgttgt taacttgttt attgcagctt ataatggtta
      421 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
      481 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggatctacg taatacgact
      541 cactatagtt ctagaggctc gacgattgaa ggttctctcc tctgggctga agaaggggtt
      601 gtgacagggc atcgcacagt tgggggtctg gccggtcctg acccggttgt acagggggtg
      661 tcgctcgtta gaaacctgca ccataggggc ccggcactgg cagcctgact cgcacccagg
      721 ggcccgggat ctgctccgcg gaggctccgc acgtgtcctg cttttaggaa ccgacctggc
      781 cgggggtttg gggggctccg ggtgactggg ggcggcggta gtctgctcag tccggttgta
      841 gtagtccata cacagggtgt ccgggttgct ttgctcgggc agacgatcac atcgcatgcg
      901 atccgg
//
