LOCUS       sequence-104213-        1059 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  docking site transgenesis plasmid for GoldenGate Cloning and insect 
            transgenesis, mTurquoise CFP marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1059)
  AUTHORS   Volohonsky G, Terenzi O, Soichot J, Naujoks DA, Nolan T, Windbichler
            N, Kapps D, Smidler AL, Vittu A, Costa G, Steinert S, Levashina EA, 
            Blandin SA, Marois E
  TITLE     Tools for Anopheles gambiae Transgenesis.
  JOURNAL   G3 (Bethesda). 2015 Apr 13. pii: g3.115.016808. doi: 
            10.1534/g3.115.016808.
  PUBMED    25869647
REFERENCE   2  (bases 1 to 1059)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2015 Apr 13. pii: g3.115.016808. doi: 
            10.1534/g3.115.016808."
COMMENT     Sequence Label: pDSAT
FEATURES             Location/Qualifiers
     source          1..1059
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     7..23
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7..23
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     28..47
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        28..46
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    77..176
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     polyA_signal    237..358
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(248..267)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     302..321
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(487..738)
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNH
                     YLSTQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     complement(529..550)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     878..897
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 agctgccagg aaacagctat gaccatgtaa tacgactcac tataggggat atcgcggccg
       61 ccctgcagct ggatggcaaa taatgatttt attttgactg atagtgacct gttcgttgca
      121 acaaattgat aagcaatgct ttcttataat gccaactttg tacaagaaag ctgggtactc
      181 gagctccacc gcggtggcgg ccgctctagg cgcgccgtac gcgtatcgat aagctttaag
      241 atacattgat gagtttggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg
      301 tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata aacaagttaa
      361 caacaacaat tgcattcatt ttatgtttca ggttcagggg gaggtgtggg aggtttttta
      421 aagcaagtaa aacctctaca aatgtggtat ggctgatttg atcacccgtc agatctatca
      481 tccggacttg tacagctcgt ccatgccgag agtgatcccg gcggcggtca cgaactccag
      541 caggaccatg tgatcgcgct tctcgttggg gtctttgctc agcttggact gggtgctcag
      601 gtagtggttg tcgggcagca gcacggggcc gtcgccgatg ggggtgttct gctggtagtg
      661 gtcggcgagc tgcacgccgc cgtcctcgat gttgtggcgg atcttgaagt tggccttgat
      721 gccgttcttc tgcttgtcgg cggtgatata gacgttgtcg ctaaagtagt tgtactccag
      781 cttgtgcccc aggatgttgc cgtcctcctt gaagtcgatg cccttcagct cgatgcggtt
      841 caccagggtg tcgccctcga acttcacctc ggcgcgggtc ttgtagttgc cgtcgtcctt
      901 gaagaagatg gtgcgctcct ggacgtagcc ttcgggcatg gcggacttga agaagtcgtg
      961 ctgcttcatg tggtcggggt agcgggcgaa gcactgcacg ccccaggaca gggtggtcac
     1021 gagggtgggc cagggcacgg gcagcttgcc ggtggtgca
//
