LOCUS       sequence-104215-        1137 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  docking site transgenesis plasmid for GoldenGate Cloning and insect 
            transgenesis, YFP marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1137)
  AUTHORS   Volohonsky G, Terenzi O, Soichot J, Naujoks DA, Nolan T, Windbichler
            N, Kapps D, Smidler AL, Vittu A, Costa G, Steinert S, Levashina EA, 
            Blandin SA, Marois E
  TITLE     Tools for Anopheles gambiae Transgenesis.
  JOURNAL   G3 (Bethesda). 2015 Apr 13. pii: g3.115.016808. doi: 
            10.1534/g3.115.016808.
  PUBMED    25869647
REFERENCE   2  (bases 1 to 1137)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2015 Apr 13. pii: g3.115.016808. doi: 
            10.1534/g3.115.016808."
COMMENT     Sequence Label: pDSAY
FEATURES             Location/Qualifiers
     source          1..1137
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     7..23
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7..23
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     28..47
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        28..46
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    77..176
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     polyA_signal    237..358
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(248..267)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     302..321
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(484..735)
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     complement(526..547)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     875..894
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 agctgccagg aaacagctat gaccatgtaa tacgactcac tataggggat atcgcggccg
       61 ccctgcagct ggatggcaaa taatgatttt attttgactg atagtgacct gttcgttgca
      121 acaaattgat aagcaatgct ttcttataat gccaactttg tacaagaaag ctgggtactc
      181 gagctccacc gcggtggcgg ccgctctagg cgcgccgtac gcgtatcgat aagctttaag
      241 atacattgat gagtttggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg
      301 tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata aacaagttaa
      361 caacaacaat tgcattcatt ttatgtttca ggttcagggg gaggtgtggg aggtttttta
      421 aagcaagtaa aacctctaca aatgtggtat ggctgattat gatctagagt cgcggccgct
      481 ttacttgtac agctcgtcca tgccgagagt gatcccggcg gcggtcacga actccagcag
      541 gaccatgtga tcgcgcttct cgttggggtc tttgctcagg gcggactggt agctcaggta
      601 gtggttgtcg ggcagcagca cggggccgtc gccgatgggg gtgttctgct ggtagtggtc
      661 ggcgagctgc acgctgccgt cctcgatgtt gtggcggatc ttgaagttca ccttgatgcc
      721 gttcttctgc ttgtcggcca tgatatagac gttgtggctg ttgtagttgt actccagctt
      781 gtgccccagg atgttgccgt cctccttgaa gtcgatgccc ttcagctcga tgcggttcac
      841 cagggtgtcg ccctcgaact tcacctcggc gcgggtcttg tagttgccgt cgtccttgaa
      901 gaagatggtg cgctcctgga cgtagccttc gggcatggcg gacttgaaga agtcgtgctg
      961 cttcatgtgg tcggggtagc gggcgaagca ctgcaggccg tagccgaagg tggtcacgag
     1021 ggtgggccag ggcacgggca gcttgccggt ggtgcagatg aacttcaggg tcagcttgcc
     1081 gtaggtggca tcgccctcgc cctcgccgga cacgctgaac ttgtggccgt ttacgtc
//
