LOCUS       sequence-104217-        1017 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  docking site transgenesis plasmid for GoldenGate Cloning and insect 
            transgenesis, DsRed RFP marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1017)
  AUTHORS   Volohonsky G, Terenzi O, Soichot J, Naujoks DA, Nolan T, Windbichler
            N, Kapps D, Smidler AL, Vittu A, Costa G, Steinert S, Levashina EA, 
            Blandin SA, Marois E
  TITLE     Tools for Anopheles gambiae Transgenesis.
  JOURNAL   G3 (Bethesda). 2015 Apr 13. pii: g3.115.016808. doi: 
            10.1534/g3.115.016808.
  PUBMED    25869647
REFERENCE   2  (bases 1 to 1017)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2015 Apr 13. pii: g3.115.016808. doi: 
            10.1534/g3.115.016808."
COMMENT     Sequence Label: pDSAR
FEATURES             Location/Qualifiers
     source          1..1017
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    210..292
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(247..266)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     302..321
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    350..419
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     regulatory      934..943
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 atctcggtct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa
       61 aatgagctga tttaacaaaa atttaacgcg ggtctccgct tctagacata atcagccata
      121 ccacatttgt agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga
      181 aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca
      241 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttcttcact gcattctagt
      301 tgtggtttgt ccaaactcat caatgtatct cgacgatgta ggtcacagtc tcgaagccgc
      361 ggtgcgggtg ccagggcgtg cccttgggct ccccgggcgc gtactccacc tcacccatct
      421 ggtccatcat gatgaacggg tcgaggtggc ggtagttgat cccggcgaac gcgcggcgca
      481 ccgggaagcc ctcgccctcg aaaccgctgg gcgcggtggt cacggtgagc acgggacgtg
      541 cgacggcgtc ggcgggtgcg gatacgcggg gcagcgtcag cgggttctcg acggtcacgg
      601 cgggcaattc ctgcagactt ccggtatctc gcgtttgttt gatcgcacgg ttcccacaat
      661 ggttaattcg agctcgcccg gggatctaat tcaattagag actaattcaa ttagagctaa
      721 ttcaattagg atccaagctt atcgatttcg aaccctcgac cgccggagta taaatagagg
      781 cgcttcgtct acggagcgac aattcaattc aaacaagcaa agtgaacacg tcgctaagcg
      841 aaagctaagc aaataaacaa gcgcagctga acaagctaaa caatcggggt accgctagag
      901 tcgacggtac cgcgggcccg ggatccaccg gtcgccacca tggtgcgctc ctccaagaac
      961 gtcatcaagg agttcatgcg cttcaaggtg cgcatggagg gcaccgtgaa cggccac
//
