LOCUS       sequence-104222-         890 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  docking site transgenesis plasmid for GoldenGate Cloning and insect 
            transgenesis, Puromycin resistance marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 890)
  AUTHORS   Volohonsky G, Terenzi O, Soichot J, Naujoks DA, Nolan T, Windbichler
            N, Kapps D, Smidler AL, Vittu A, Costa G, Steinert S, Levashina EA, 
            Blandin SA, Marois E
  TITLE     Tools for Anopheles gambiae Transgenesis.
  JOURNAL   G3 (Bethesda). 2015 Apr 13. pii: g3.115.016808. doi: 
            10.1534/g3.115.016808.
  PUBMED    25869647
REFERENCE   2  (bases 1 to 890)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2015 Apr 13. pii: g3.115.016808. doi: 
            10.1534/g3.115.016808."
COMMENT     Sequence Label: pDSAP
FEATURES             Location/Qualifiers
     source          1..890
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     7..23
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7..23
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     28..47
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        28..46
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    77..176
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     polyA_signal    235..369
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(259..278)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     313..332
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(464..484)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
ORIGIN
        1 agctgccagg aaacagctat gaccatgtaa tacgactcac tataggggat atcgcggccg
       61 ccctgcagct ggatggcaaa taatgatttt attttgactg atagtgacct gttcgttgca
      121 acaaattgat aagcaatgct ttcttataat gccaactttg tacaagaaag ctgggtactc
      181 gagctccacc gcggtggcgg ccgctctagg cgcgccgtac cgggcccccc ccatgatcca
      241 gacatgataa gatacattga tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa
      301 tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat
      361 aaacaagttt cgaggtcgag tcaggcaccg ggcttgcggg tcatgcacca ggtgcgcggt
      421 ccttcgggca cctcgacgtc ggcggtgacg gtgaagccga gccgctcgta gaaggggagg
      481 ttgcggggcg cggaggtctc caggaaggcg ggcaccccgg cgcgctcggc cgcctccact
      541 ccggggagca cgacggcgct gcccagaccc ttgccctggt ggtcgggcga gacgccgacg
      601 gtggccagga accacgcggg ctccttgggc cggtgcggcg ccaggaggcc ttccatctgt
      661 tgctgcgcgg ccagccggga accgctcaac tcggccatgc gcgggccgat ctcggcgaac
      721 accgcccccg cttcgacgct ctccggcgtg gtccagaccg ccaccgcggc gccgtcgtcc
      781 gcgacccaca ccttgccgat gtcgagcccg acgcgcgtga ggaagagttc ttgcagctcg
      841 gtgacccgct cgatgtggcg gtccggatcg acggtgtggc gcgtggcggg
//
