LOCUS       sequence-10423-e        9367 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9367)
  AUTHORS   Zhang YP, Holbro N, Oertner TG
  TITLE     Optical induction of plasticity at single synapses reveals 
            input-specific accumulation of alphaCaMKII.
  JOURNAL   Proc Natl Acad Sci U S A. 2008 Aug 19. 105(33):12039-44.
  PUBMED    18697934
REFERENCE   2  (bases 1 to 9367)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2008 Aug 19. 105(33):12039-44."
COMMENT     Sequence Label: pMH4-SYN-ChR2-tdimer2RFP
FEATURES             Location/Qualifiers
     source          1..9367
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_signal     191..341
                     /label=Ad5 Psi
                     /note="packaging signal for adenovirus serotype 5"
     promoter        399..846
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     regulatory      864..873
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             870..1796
                     /codon_start=1
                     /product="N-terminal fragment of channelrhodopsin-2 from 
                     the alga Chlamydomonas reinhardtii (Nagel et al., 2003)"
                     /label=ChR2
                     /note="blue light-activated cation channel"
                     /translation="MDYGGALSAVGRELLFVTNPVVVNGSVLVPEDQCYCAGWIESRGT
                     NGAQTASNVLQWLAAGFSILLLMFYAYQTWKSTCGWEEIYVCAIEMVKVILEFFFEFKN
                     PSMLYLATGHRVQWLRYAEWLLTCPVILIHLSNLTGLSNDYSRRTMGLLVSDIGTIVWG
                     ATSAMATGYVKVIFFCLGLCYGANTFFHAAKAYIEGYHTVPKGRCRQVVTGMAWLFFVS
                     WGMFPILFILGPEGFGVLSVYGSTVGHTIIDLMSKNCWGLLGHYLRVLIHEHILIHGDI
                     RKTTKLNIGGTEIEVETLVEDEAEAGAV"
     regulatory      1803..1812
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      1807..1816
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1809..2486
                     /codon_start=1
                     /product="rapidly maturing tetrameric variant of DsRed 
                     fluorescent protein (Bevis and Glick, 2002)"
                     /label=DsRed-Express
                     /note="mammalian codon-optimized"
                     /translation="MVASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     VVTVTQDSSLQDGTLIYKVKFRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFL"
     CDS             2529..3203
                     /codon_start=1
                     /product="rapidly maturing tetrameric variant of DsRed 
                     fluorescent protein (Bevis and Glick, 2002)"
                     /label=DsRed-Express
                     /note="mammalian codon-optimized"
                     /translation="ASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAKL
                     KVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGVV
                     TVTQDSSLQDGTLIYKVKFRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIHQA
                     LKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRHHL
                     FL"
     polyA_signal    3217..3351
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3254..3273)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3308..3327
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             6898..7089
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     complement(7083..7105)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    7191..7331
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     7246..7265
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(7346..7363)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7517..8105)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7597..7616)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8276..9067,9068..9136))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8899..8918
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9137..9241)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9309..9327
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcccttccag ctctctgccc cttttggatt gaagccaata tgataatgag ggggtggagt
       61 ttgtgacgtg gcgcggggcg tgggaacggg gcgggtgacg tagtagtgtg gcggaagtgt
      121 gatgttgcaa gtgtggcgga acacatgtaa gcgacggatg tggcaaaagt gacgtttttg
      181 gtgtgcgccg gtgtacacag gaagtgacaa ttttcgcgcg gttttaggcg gatgttgtag
      241 taaatttggg cgtaaccgag taagatttgg ccattttcgc gggaaaactg aataagagga
      301 agtgaaatct gaataatttt gtgttactca tagcgcgtaa tctctagcat cgaagatccc
      361 tcgactctag actcgagctg cagagggccc tgcgtatgag tgcaagtggg ttttaggacc
      421 aggatgaggc ggggtggggg tgcctacctg acgaccgacc ccgacccact ggacaagcac
      481 ccaaccccca ttccccaaat tgcgcatccc ctatcagaga gggggagggg aaacaggatg
      541 cggcgaggcg cgtgcgcact gccagcttca gcaccgcgga cagtgccttc gcccccgcct
      601 ggcggcgcgc gccaccgccg cctcagcact gaaggcgcgc tgacgtcact cgccggtccc
      661 ccgcaaactc cccttcccgg ccaccttggt cgcgtccgcg ccgccgccgg cccagccgga
      721 ccgcaccacg cgaggcgcga gatagggggg cacgggcgcg accatctgcg ctgcggcgcc
      781 ggcgactcag cgctgcctca gtctgcggtg ggcagcggag gagtcgtgtc gtgcctgaga
      841 gcgcagtcga attcaagctg ctagccacca tggattatgg aggcgccctg agtgccgttg
      901 ggcgcgagct gctatttgta acgaacccag tagtcgtcaa tggctctgta cttgtgcctg
      961 aggaccagtg ttactgcgcg ggctggattg agtcgcgtgg cacaaacggt gcccaaacgg
     1021 cgtcgaacgt gctgcaatgg cttgctgctg gcttctccat cctactgctt atgttttacg
     1081 cctaccaaac atggaagtca acctgcggct gggaggagat ctatgtgtgc gctatcgaga
     1141 tggtcaaggt gattctcgag ttcttcttcg agtttaagaa cccgtccatg ctgtatctag
     1201 ccacaggcca ccgcgtccag tggttgcgtt acgccgagtg gcttctcacc tgcccggtca
     1261 ttctcattca cctgtcaaac ctgacgggct tgtccaacga ctacagcagg cgcaccatgg
     1321 gtctgcttgt gtctgatatt ggcacaattg tgtggggcgc cacttccgcc atggccaccg
     1381 gatacgtcaa ggtcatcttc ttctgcctgg gtctgtgtta tggtgctaac acgttctttc
     1441 acgctgccaa ggcctacatc gagggttacc acaccgtgcc gaagggccgg tgtcgccagg
     1501 tggtgactgg catggcttgg ctcttcttcg tatcatgggg tatgttcccc atcctgttca
     1561 tcctcggccc cgagggcttc ggcgtcctga gcgtgtacgg ctccaccgtc ggccacacca
     1621 tcattgacct gatgtcgaag aactgctggg gtctgctcgg ccactacctg cgcgtgctga
     1681 tccacgagca tatcctcatc cacggcgaca ttcgcaagac caccaaattg aacattggtg
     1741 gcactgagat tgaggtcgag acgctggtgg aggacgaggc cgaggctggc gcggtacccg
     1801 cggccgccat ggtggcctcc tccgaggacg tcatcaaaga gttcatgcgc ttcaaggtgc
     1861 gcatggaggg ctccgtgaac ggccacgagt tcgagatcga gggcgagggc gagggccgcc
     1921 cctacgaggg cacccagacc gccaagctga aggtgaccaa gggcggcccc ctgcccttcg
     1981 cctgggacat cctgtccccc cagttccagt acggctccaa ggcgtacgtg aagcaccccg
     2041 ccgacatccc cgactacaag aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga
     2101 tgaacttcga ggacggcggc gtggtgaccg tgacccagga ctcctccctg caggacggca
     2161 cgctgatcta caaggtgaag ttccgcggca ccaacttccc ccccgacggc cccgtaatgc
     2221 agaagaagac catgggctgg gaggcctcca ccgagcgcct gtacccccgc gacggcgtgc
     2281 tgaagggcga gatccaccag gccctgaagc tgaaggacgg cggccactac ctggtggagt
     2341 tcaagaccat ctacatggcc aagaagcccg tgcagctgcc cggctactac tacgtggaca
     2401 ccaagctgga catcacctcc cacaacgagg actacaccat cgtggaacag tacgagcgct
     2461 ccgagggccg ccaccacctg ttcctggggc atggcaccgg cagcaccggc agcggcagct
     2521 ccggcaccgc ctcctccgag gacgtcatca aagagttcat gcgcttcaag gtgcgcatgg
     2581 agggctccgt gaacggccac gagttcgaga tcgagggcga gggcgagggc cgcccctacg
     2641 agggcaccca gaccgccaag ctgaaggtga ccaagggcgg ccccctgccc ttcgcctggg
     2701 acatcctgtc cccccagttc cagtacggct ccaaggcgta cgtgaagcac cccgccgaca
     2761 tccccgacta caagaagctg tccttccccg agggcttcaa gtgggagcgc gtgatgaact
     2821 tcgaggacgg cggcgtggtg accgtgaccc aggactcctc cctgcaggac ggcacgctga
     2881 tctacaaggt gaagttccgc ggcaccaact tcccccccga cggccccgta atgcagaaga
     2941 agaccatggg ctgggaggcc tccaccgagc gcctgtaccc ccgcgacggc gtgctgaagg
     3001 gcgagatcca ccaggccctg aagctgaagg acggcggcca ctacctggtg gagttcaaga
     3061 ccatctacat ggccaagaag cccgtgcagc tgcccggcta ctactacgtg gacaccaagc
     3121 tggacatcac ctcccacaac gaggactaca ccatcgtgga acagtacgag cgctccgagg
     3181 gccgccacca cctgttcctg taggtcgact tcgagcaact tgtttattgc agcttataat
     3241 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
     3301 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggat cgtctagcat
     3361 cgaagatctg ggcgtggtta agggtgggaa agaatatata aggtgggggt cttatgtagt
     3421 tttgtatctg ttttgcagca gccgccgccg ccatgagcac caactcgttt gatggaagca
     3481 ttgtgagctc atatttgaca acgcgcatgc ccccatgggc cggggtgcgt cagaatgtga
     3541 tgggctccag cattgatggt cgccccgtcc tgcccgcaaa ctctactacc ttgacctacg
     3601 agaccgtgtc tggaacgccg ttggagactg cagcctccgc cgccgcttca gccgctgcag
     3661 ccaccgcccg cgggattgtg actgactttg ctttcctgag cccgcttgca agcagtgcag
     3721 cttcccgttc atccgcccgc gatgacaagt tgacggctct tttggcacaa ttggattctt
     3781 tgacccggga acttaatgtc gtttctcagc agctgttgga tctgcgccag caggtttctg
     3841 ccctgaaggc ttcctcccct cccaatgcgg tttaaaacat aaataaaaaa ccagactctg
     3901 tttggatttg gatcaagcaa gtgtcttgct gtctttattt aggggttttg cgcgcgcggt
     3961 aggcccggga ccagcggtct cggtcgttga gggtcctgtg tattttttcc aggacgtggt
     4021 aaaggtgact ctggatgttc agatacatgg gcataagccc gtctctgggg tggaggtagc
     4081 accactgcag agcttcatgc tgcggggtgg tgttgtagat gatccagtcg tagcaggagc
     4141 gctgggcgtg gtgcctaaaa atgtctttca gtagcaagct gattgccagg ggcaggccct
     4201 tggtgtaagt gtttacaaag cggttaagct gggatgggtg catacgtggg gatatgagat
     4261 gcatcttgga ctgtattttt aggttggcta tgttcccagc catatccctc cggggattca
     4321 tgttgtgcag aaccaccagc acagtgtatc cggtgcactt gggaaatttg tcatgtagct
     4381 tagaaggaaa tgcgtggaag aacttggaga cgcccttgtg acctccaaga ttttccatgc
     4441 attcgtccat aatgatggca atgggcccac gggcggcggc ctgggcgaag atatttctgg
     4501 gatcactaac gtcatagttg tgttccagga tgagatcgtc ataggccatt tttacaaagc
     4561 gcgggcggag ggtgccagac tgcggtataa tggttccatc cggcccaggg gcgtagttac
     4621 cctcacagat ttgcatttcc cacgctttga gttcagatgg ggggatcatg tctacctgcg
     4681 gggcgatgaa gaaaacggtt tccggggtag gggagatcag ctgggaagaa agcaggttcc
     4741 tgagcagctg cgacttaccg cagccggtgg gcccgtaaat cacacctatt accgggtgca
     4801 actggtagtt aagagagctg cagctgccgt catccctgag caggggggcc acttcgttaa
     4861 gcatgtccct gactcgcatg ttttccctga ccaaatccgc cagaaggcgc tcgccgccca
     4921 gcgatagcag ttcttgcaag gaagcaaagt ttttcaacgg tttgagaccg tccgccgtag
     4981 gcatgctttt gagcgtttga ccaagcagtt ccaggcggtc ccacagctcg gtcacctgct
     5041 ctacggcatc tcgatccagc atatctcctc gtttcgcggg ttggggcggc tttcgctgta
     5101 cggcagtagt cggtgctcgt ccagacgggc cagggtcatg tctttccacg ggcgcagggt
     5161 cctcgtcagc gtagtctggg tcacggtgaa ggggtgcgct ccgggctgcg cgctggccag
     5221 ggtgcgcttg aggctggtcc tgctggtgct gaagcgctgc cggtcttcgc cctgcgcgtc
     5281 ggccaggtag catttgacca tggtgtcata gtccagcccc tccgcggcgt ggcccttggc
     5341 gcgcagcttg cccttggagg aggcgccgca cgaggggcag tgcagacttt tgagggcgta
     5401 gagcttgggc gcgagaaata ccgattccgg ggagtaggca tccgcgccgc aggccccgca
     5461 gacggtctcg cattccacga gccaggtgag ctctggccgt tcggggtcaa aaaccaggtt
     5521 tcccccatgc tttttgatgc gtttcttacc tctggtttcc atgagccggt gtccacgctc
     5581 ggtgacgaaa aggctgtccg tgtccccgta tacagacttg agaggcctgt cctcgaccga
     5641 tgcccttgag agccttcaac ccagtcagct ccttccggtg ggcgcggggc atgactatcg
     5701 tcgccgcact tatgactgtc ttctttatca tgcaactcgt aggacaggtg ccggcagcgc
     5761 tctgggtcat tttcggcgag gaccgctttc gctggagcgc gacgatgatc ggcctgtcgc
     5821 ttgcggtatt cggaatcttg cacgccctcg ctcaagcctt cgtcactggt cccgccacca
     5881 aacgtttcgg cgagaagcag gccattatcg ccggcatggc ggccgacgcg ctgggctacg
     5941 tcttgctggc gttcgcgacg cgaggctgga tggccttccc cattatgatt cttctcgctt
     6001 ccggcggcat cgggatgccc gcgttgcagg ccatgctgtc caggcaggta gatgacgacc
     6061 atcagggaca gcttcaagga tcgctcgcgg ctcttaccag cctaacttcg atcactggac
     6121 cgctgatcgt cacggcgatt tatgccgcct cggcgagcac atggaacggg ttggcatgga
     6181 ttgtaggcgc cgccctatac cttgtctgcc tccccgcgtt gcgtcgcggt gcatggagcc
     6241 gggccacctc gacctgaatg gaagccggcg gcacctcgct aacggattca ccactccaag
     6301 aattggagcc aatcaattct tgcggagaac tgtgaatgcg caaaccaacc cttggcagaa
     6361 catatccatc gcgtccgcca tctccagcag ccgcacgcgg cgcatctcgg gcagcgttgg
     6421 gtcctggcca cgggtgcgca tgatcgtgct cctgtcgttg aggacccggc taggctggcg
     6481 gggttgcctt actggttagc agaatgaatc accgatacgc gagcgaacgt gaagcgactg
     6541 ctgctgcaaa acgtctgcga cctgagcaac aacatgaatg gtcttcggtt tccgtgtttc
     6601 gtaaagtctg gaaacgcgga agtcagcgcc ctgcaccatt atgttccgga tctgcatcgc
     6661 aggatgctgc tggctaccct gtggaacacc tacatctgta ttaacgaagc gctggcattg
     6721 accctgagtg atttttctct ggtcccgccg catccatacc gccagttgtt taccctcaca
     6781 acgttccagt aaccgggcat gttcatcatc agtaacccgt atcgtgagca tcctctctcg
     6841 tttcatcggt atcattaccc ccatgaacag aaattccccc ttacacggag gcatcaagtg
     6901 accaaacagg aaaaaaccgc ccttaacatg gcccgcttta tcagaagcca gacattaacg
     6961 cttctggaga aactcaacga gctggacgcg gatgaacagg cagacatctg tgaatcgctt
     7021 cacgaccacg ctgatgagct ttaccgcagc tgcctcgcgc gtttcggtga tgacggtgaa
     7081 aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg
     7141 agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg cgcagccatg
     7201 acccagtcac gtagcgatag cggagtgtat actggcttaa ctatgcggca tcagagcaga
     7261 ttgtactgag agtgcaccat atgcggtgtg aaataccgca cagatgcgta aggagaaaat
     7321 accgcatcag gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     7381 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     7441 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     7501 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     7561 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     7621 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     7681 ttctcccttc gggaagcgtg gcgctttctc aatgctcacg ctgtaggtat ctcagttcgg
     7741 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     7801 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     7861 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     7921 tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc
     7981 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     8041 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     8101 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     8161 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     8221 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     8281 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     8341 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     8401 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     8461 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     8521 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     8581 ttgccattgc tgcaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     8641 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     8701 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     8761 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     8821 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     8881 gcccggcgtc aacacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     8941 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     9001 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     9061 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     9121 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
     9181 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
     9241 gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa
     9301 cctataaaaa taggcgtatc acgaggccct ttcgtcttca agaattgatc cgggccccca
     9361 tttcccc
//
