LOCUS       sequence-104231-         850 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd gen lentiviral Luciferase expression vector, CMV promoter, Puro.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 850)
  AUTHORS   Campeau E, Ruhl VE, Rodier F, Smith CL, Rahmberg BL, Fuss JO, 
            Campisi J, Yaswen P, Cooper PK, Kaufman PD.
  TITLE     A versatile viral system for expression and depletion of proteins in
            mammalian cells.
  JOURNAL   PLoS One. 2009 Aug 6;4(8):e6529.
  PUBMED    19657394
REFERENCE   2  (bases 1 to 850)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2009-08-6"; volume: "4(8)"; pages: "e6529"
COMMENT     Sequence Label: pLenti CMV Puro LUC (w168-1)
FEATURES             Location/Qualifiers
     source          1..850
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    55..79
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 aattttgtaa tccagaggtt gattgtcgag cggccgccac tgtgctggat atcaaccact
       61 ttgtacaaga aagctgggtc tagaattaca cggcgatctt tccgcccttc ttggccttta
      121 tgaggatctc tctgattttt cttgcgtcga gttttccggt aagacctttc ggtacttcgt
      181 ccacaaacac aactcctccg cgcaactttt tcgcggttgt tacttgactg gcgacgtaat
      241 ccacgatctc tttttccgtc atcgtctttc cgtgctccaa aacaacaacg gcggcgggaa
      301 gttcaccggc gtcatcgtcg ggaagacctg cgacacctgc gtcgaagatg ttggggtgtt
      361 ggagcaagat ggattccaat tcagcgggag ccacctgata gcctttgtac ttaatcagag
      421 acttcaggcg gtcaacgatg aagaagtgtt cgtcttcgtc ccagtaagct atgtctccag
      481 aatgtagcca tccatccttg tcaatcaagg cgttggtcgc ttccggattg tttacataac
      541 cggacataat cataggacct ctcacacaca gttcgcctct ttgattaacg cccagcgttt
      601 tcccggtatc cagatccaca accttcgctt caaaaaatgg aacaacttta ccgaccgcgc
      661 ccggtttatc atccccctcg ggtgtaatca gaatagctga tgtagtctca gtgagcccat
      721 atccttgcct gatacctggc agatggaacc tcttggcaac cgcttccccg acttccttag
      781 agaggggagc gccaccagaa gcaatttcgt gtaaattaga taaatcgtat ttgtcaatca
      841 gagtgctttt
//
