LOCUS       sequence-104233-         886 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of human Akt1 with myr, HA tags.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 886)
  AUTHORS   Ramaswamy S, Nakamura N, Vazquez F, Batt DB, Perera S, Roberts TM, 
            Sellers WR
  TITLE     Regulation of G1 progression by the PTEN tumor suppressor protein is
            linked to inhibition of the phosphatidylinositol 3-kinase/Akt 
            pathway.
  JOURNAL   Proc Natl Acad Sci U S A. 1999 Mar 2. 96(5):2110-5.
  PUBMED    10051603
REFERENCE   2  (bases 1 to 886)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 1999 Mar 2. 96(5):2110-5."
COMMENT     Sequence Label: 1036 pcDNA3 Myr HA Akt1
FEATURES             Location/Qualifiers
     source          1..886
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     49..68
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        49..67
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             90..131
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="MGSSKSKPKDPSQR"
     CDS             135..161
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YAYDVPDYA"
     CDS             141..167
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
ORIGIN
        1 agctctctgg ctaactagag aacccactgc ttactggctt atcgaaatta atacgactca
       61 ctatagggag acccaagctt cccaccacca tggggagcag caagagcaag cccaaggacc
      121 ccagccagcg cgcctacgcc tacgacgtgc ccgactacgc ctccctcgga tccatgagcg
      181 acgtggctat tgtgaaggag ggttggctgc acaaacgagg ggagtacatc aagacctggc
      241 ggccacgcta cttcctcctc aagaatgatg gcaccttcat tggctacaag gagcggccgc
      301 aggatgtgga ccaacgtgag gctcccctca acaacttctc tgtggcgcag tgccagctga
      361 tgaagacgga gcggccccgg cccaacacct tcatcatccg ctgcctgcag tggaccactg
      421 tcatcgaacg caccttccat gtggagactc ctgaggagcg ggaggagtgg acaaccgcca
      481 tccagactgt ggctgacggc ctcaagaagc aggaggagga ggagatggac ttccggtcgg
      541 gctcacccag tgacaactca ggggctgaag agatggaggt gtccctggcc aagcccaagc
      601 accgcgtgac catgaacgag tttgagtacc tgaagctgct gggcaagggc actttcggca
      661 aggtgatcct ggtgaaggag aaggccacag gccgctacta cgccatgaag atcctcaaga
      721 aggaagtcat cgtggccaag gacgaggtgg cccacacact caccgagaac cgcgtcctgc
      781 agaactccag gcaccccttc ctcacagccc tgaagtactc tttccagacc cacgaccgcc
      841 tctgctttgt catggagtac gccaacgggg gcgagctgtt cttcca
//
