LOCUS       sequence-104236-         709 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression plasmid for use in structural determination..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 709)
  AUTHORS   Kaustov L, Ouyang H, Amaya M, Lemak A, Nady N, Duan S, Wasney GA, Li
            Z, Vedadi M, Schapira M, Min J, Arrowsmith CH
  TITLE     Recognition and specificity determinants of the human cbx 
            chromodomains.
  JOURNAL   J Biol Chem. 2011 Jan 7;286(1):521-9. doi: 10.1074/jbc.M110.191411. 
            Epub 2010 Nov 3.
  PUBMED    21047797
REFERENCE   2  (bases 1 to 709)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M110.191411"; journalName: "J Biol Chem"; date: 
            "2011-01-7- 7"; volume: "286"; issue: "1"; pages: "521-9"
COMMENT     Sequence Label: 3I91
FEATURES             Location/Qualifiers
     source          1..709
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             13..30
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             43..63
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             255..272
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(325..343)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      339..386
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(510..529)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 agatatacca tgcatcatca tcatcatcac agcagcggca gagaaaactt gtatttccag
       61 ggcgagcggg tgttcgcggc cgaagccctc ctgaagcggc gcatacggaa aggacgcatg
      121 gaatacctcg tgaaatggaa gggatggtcg cagaagtaca gcacatggga accggaggaa
      181 aacatcctgg atgctcgctt gctcgcagcc tttgaggaaa gggaatgatg acgaagcttg
      241 cggccgcact cgagcaccac caccaccacc actgagatcc ggctgctaac aaagcccgaa
      301 aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct
      361 ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga ttggcgaatg
      421 ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
      481 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
      541 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
      601 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg
      661 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacg
//
