LOCUS       sequence-104237-         651 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression plasmid for use in structural determination..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 651)
  AUTHORS   Kaustov L, Ouyang H, Amaya M, Lemak A, Nady N, Duan S, Wasney GA, Li
            Z, Vedadi M, Schapira M, Min J, Arrowsmith CH
  TITLE     Recognition and specificity determinants of the human cbx 
            chromodomains.
  JOURNAL   J Biol Chem. 2011 Jan 7;286(1):521-9. doi: 10.1074/jbc.M110.191411. 
            Epub 2010 Nov 3.
  PUBMED    21047797
REFERENCE   2  (bases 1 to 651)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M110.191411"; journalName: "J Biol Chem"; date: 
            "2011-01-7- 7"; volume: "286"; issue: "1"; pages: "521-9"
COMMENT     Sequence Label: 3I91
FEATURES             Location/Qualifiers
     source          1..651
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(16..33)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(225..245)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             complement(258..275)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             285..307
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    322..346
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(346..365)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(347..365)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(449..468)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 agcagccgga tctcagtggt ggtggtggtg gtgctcgagt gcggccgcaa gcttcgtcat
       61 cattcccttt cctcaaaggc tgcgagcaag cgagcatcca ggatgttttc ctccggttcc
      121 catgtgctgt acttctgcga ccatcccttc catttcacga ggtattccat gcgtcctttc
      181 cgtatgcgcc gcttcaggag ggcttcggcc gcgaacaccc gctcgccctg gaaatacaag
      241 ttttctctgc cgctgctgtg atgatgatga tgatgcatgg tatatctcct tcttaaagtt
      301 aaacaaaatt atttctagag gggaattgtt atccgctcac aattccccta tagtgagtcg
      361 tattaatttc gcgggatcga gatctcgatc ctctacgccg gacgcatcgt ggccggcatc
      421 accggcgcca caggtgcggt tgctggcgcc tatatcgccg acatcaccga tggggaagat
      481 cgggctcgcc acttcgggct catgagcgct tgtttcggcg tgggtatggt ggcaggcccc
      541 gtggccgggg gactgttggg cgccatctcc ttgcatgcac cattccttgc ggcggcggtg
      601 ctcaacggcc tcaacctact actgggctgc ttcctaatgc aggagtcgca t
//
