LOCUS       sequence-104238-         686 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  E. coli expression vector with N-terminal AviTag and a C-terminal 
            Hexa-HIS tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 686)
  TITLE     Arrowsmith Lab Plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 686)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: p28BIOH-LIC
FEATURES             Location/Qualifiers
     source          1..686
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(7..24)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(54..71)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 atctcagtgg tggtggtggt ggtgctcgag tgcggccgca agcttcgtca tcagtgatga
       61 tggtggtgat gaccacttcc acctgtgcat gatctcctcg aaaagtgcca cctgacgtca
      121 atgccaatag gatatcggca ttttcttttg cgtttttatt tgttaactgt taattgtcct
      181 tgttcaagga tgctgtcttt gacaacagat gttttcttgc ctttgatgtt cagcaggaag
      241 ctaggcgcaa acgttgattg tttgtctgcg tagaatcctc tgtttgtcat atagcttgta
      301 atcacgacat tgtttccttt cgcttgaggt acagcgaagt gtgagtaagt aaaggttaca
      361 tcgttaggat caagatccat ttttaacaca aggccagttt tgttcagcgg cttgtatggg
      421 ccagttaaag aattagaaac ataaccaagc atgtaaatat cgttagacgt aatgccgtca
      481 atcgtcattt ttgatccgcg ggagtcagtg aacaggtacc atttgccgtt cattttaaag
      541 acgttcgcgc gttcaatttc atctgttact gtgttagatg caatcagcgg tttcatcact
      601 tttttcagtg tgtaatcatc gtttagctca atcataccga gagcgccgtt tgctaactca
      661 gccgtgcgtt ttttatcgct ttgcag
//
